Review
Ophthalmology
Steven E. Wilson
Summary: This review highlights the interactions and regulatory roles of interleukin (IL)-1 and transforming growth factor (TGF) beta in the corneal wound healing process. Both systems play crucial roles in regulating cellular functions and gene expressions during the healing response to injuries or infections, and their coordinated downregulation is necessary to terminate the response and prevent fibrosis in the cornea. Understanding the IL-1 and TGF beta systems better can lead to improved approaches for controlling excessive healing responses and scarring corneal fibrosis caused by infections and injuries.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Piotr Kmiec, Stephan Rosenkranz, Margarete Odenthal, Evren Caglayan
Summary: Angiotensin II, a major factor in cardiovascular disease, activates mediators that are involved in pathological cardiac remodeling. In this study, the effects of aldosterone and transforming growth factor beta-1 (TGF-beta-1) were investigated in an in vivo model. The findings suggest that aldosterone induces cardiac hypertrophy independently of TGF-beta-1, while both factors may converge in the signaling pathways related to fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Xinyi Wang, Ting Liu, Yifei Huang, Yifeng Dai, Hui Lin
Summary: Fibrosis is a complex abnormal healing process that involves multiple signaling pathways, with the TGF-beta signaling pathway playing a central role. TGF-beta precisely regulates fibrogenesis by controlling gene transcription and translation, and SUMOylation has been identified as a key player in this process, providing potential therapeutic strategies for fibrosis.
Article
Biochemistry & Molecular Biology
Beatriz Ballester, Javier Milara, Paula Montero, Julio Cortijo
Summary: The study reveals the overexpression of MUC16 in lung tissue of IPF patients and its collaboration with the TGF-beta 1 pathway to induce fibrotic processes. Repression of MUC16 expression can inhibit fibrotic processes, suggesting that targeting MUC16 may be a potential drug target for human IPF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medicine, Research & Experimental
Savalan Babapoor-Farrokhran, Roozbeh Tarighati Rasekhi, Deanna Gill, Jafar Alzubi, Sumeet K. Mainigi
Summary: Atrial fibrillation is a common clinically significant arrhythmia with four fundamental pathophysiological mechanisms, with TGF-beta playing a key role. Studies have shown that TGF-beta 1 is involved in the development and propagation of atrial fibrillation.
Article
Gastroenterology & Hepatology
Ming-Yu Zhou, Ming-Liang Cheng, Tao Huang, Rui-Han Hu, Gao-Liang Zou, Hong Li, Bao-Fang Zhang, Juan-Juan Zhu, Yong-Mei Liu, Yang Liu, Xue-Ke Zhao
Summary: The study found that TGF-beta 1 induces GLUT1 expression in HSCs and is involved in the development of liver fibrosis. Experimental results showed that GLUT1 inhibition can reduce the degree of liver fibrosis. The mechanisms of action of GLUT1, TGF-beta 1, and aerobic glycolysis in the process of HSC activation during liver fibrosis were explored through various experimental approaches.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Chemistry, Medicinal
Manman Ma, Xiaohua Wang, Xiaohui Liu, Yang Han, Yanhui Chu, Yanzhong Guan, Haifeng Liu
Summary: A novel tT beta RII variant, Z-tT beta RII, was designed by fusing the PDGF beta receptor-specific affibody Z(PDGF beta R) to the N-terminus of tT beta RII. Z-tT beta RII exhibited superior specific fibrotic liver-targeting potential and stronger anti-fibrotic effects in vitro and in vivo. It also showed no significant sign of potential side effects in other vital organs in liver fibrotic mice, making it a potential candidate for targeted therapy for liver fibrosis.
ARCHIVES OF PHARMACAL RESEARCH
(2023)
Article
Medicine, Research & Experimental
Wanangkan Poolsri, Rattikarn Noitem, Promsuk Jutabha, Manasanan Raveesunthornkiat, Ade Danova, Warinthorn Chavasiri, Chatchai Muanprasat
Summary: This study identified a novel anti-fibrotic agent, AD-021, which effectively reduced collagen accumulation in renal proximal tubule epithelial cells (RPTEC) by inhibiting the TGF-beta 1 pathway. AD-021 suppressed TGF-beta 1-induced collagen production and activation of pro-fibrotic proteins, and ameliorated mitochondrial fission in RPTEC cells. In a mouse model of renal fibrosis, AD-021 reduced plasma TGF-beta 1, improved renal fibrosis, and enhanced renal function.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Medicine, Research & Experimental
Chung -Yu Chen, Hsuan-Yin Tung, Yu-Fang Tseng, Jau-Shyang Huang, Li-Shian Shi, Yi-Ling Ye
Summary: VB and isoVB show potential as anti-fibrotic agents by inhibiting TGF-beta 1-induced collagen I expression through oxidative stress inhibition and downregulation of the Smad/non-Smad pathway.
Article
Biochemistry & Molecular Biology
Fuensanta Gomez-Bernal, Juan Carlos Quevedo-Abeledo, Maria Garcia-Gonzalez, Yolanda Fernandez-Cladera, Agustin F. Gonzalez-Rivero, Antonia de Vera-Gonzalez, Candelaria Martin-Gonzalez, Miguel A. Gonzalez-Gay, Ivan Ferraz-Amaro
Summary: This study aimed to investigate the relationship between serum levels of transforming growth factor beta 1 (TGF-β1) and disease characteristics, activity, damage, or severity in patients with systemic lupus erythematosus (SLE). The results showed that TGF-β1 serum levels were associated with disease severity and activity, with ocular and cardiovascular manifestations positively correlated, while gastrointestinal and musculoskeletal involvements negatively correlated with circulating TGF-β1 levels.
Article
Food Science & Technology
Hui Wang, Zhe Yang, Kang Chen, Ruilian Yu, Li Xu, Gaohong Lv
Summary: This study explores the potential therapeutic effect and mechanisms of artificial Isaria cicadae Miquel (IcM) in Crohn's disease (CD)-related intestinal fibrosis. The results show that IcM can prevent intestinal fibrosis, down-regulate the expression of fibrosis markers, and promote the expression of antifibrotic factors.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Hematology
Ryosuke Taniguchi, Yuichi Ohashi, Jung Seok Lee, Haidi Hu, Luis Gonzalez, Weichang Zhang, John Langford, Yutaka Matsubara, Bogdan Yatsula, George Tellides, Tarek M. Fahmy, Katsuyuki Hoshina, Alan Dardik
Summary: Endothelial cell-targeted TGF-beta inhibition may be a translational strategy to improve AVF patency.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Integrative & Complementary Medicine
Xin Juan-juan, Gao Jun-hong, Liu Qun, Zhao Yu-xue, Zhou Chen, Yu Xiao-chun
Summary: This study showed that electroacupuncture at Neiguan (PC 6) can alleviate cardiac fibrosis in spontaneously hypertensive rats (SHRs). This effect may be mediated through the regulation of interleukin-1 beta/insulin-like growth factor 1-transforming growth factor beta 1-matrix metalloproteinase 9 pathway.
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
T. Xing, Z. R. Zhao, X. Zhao, X. L. Xu, L. Zhang, F. Gao
Summary: Wooden breast myopathy in broiler chickens induces severe fibrosis in the pectoralis major muscle by enhancing extracellular matrix deposition, collagen cross-linking, and dysregulating TGF-beta signaling and the MMP and TIMP system.
Review
Biochemistry & Molecular Biology
Zhuo Chen, Hanjie Yu, Xiangqin Chen, Wentian Chen, Wanghua Song, Zheng Li
Summary: TGF-beta superfamily members play important roles in various diseases, and glycosylation modification is involved in the regulation of TGF-beta signaling pathway. There is a complex mutual regulation between glycosylation and TGF-beta signaling, which affects biological processes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Cell Biology
Helene Juul Belling, Wolfgang Hofmeister, Ditte Caroline Andersen
Article
Cell & Tissue Engineering
Mads Gustaf Jorgensen, Navid Mohamadpour Toyserkani, Charlotte Harken Jensen, Ditte Caroline Andersen, Soren Paludan Sheikh, Jens Ahm Sorensen
Summary: The use of adipose-derived regenerative cells (ADRCs) combined with fat grafting for treating breast cancer-related lymphedema (BCRL) patients is safe and effective in clinical application. Although there was no significant reduction in BCRL volume, some patients showed improvement in symptoms, and a few felt substantial improvement in their condition.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Rheumatology
Sussi B. Mortensen, Ann-Brit E. Hansen, Trine H. Mogensen, Marianne A. Jakobsen, Hans C. Beck, Eva B. Harvald, Kate L. Lambertsen, Isik S. Johansen, Ditte C. Andersen
Summary: The study showed a specific lack of IL-1Ra expression in pyrin inflammasome activation, suggesting a new mechanism for FMF pathogenesis. Reduced IL-1Ra levels in FMF monocytes indicate a diminished anti-inflammatory capacity, potentially increasing sensitivity to pro-inflammatory stimuli in FMF patients, independent of colchicine therapy. Further investigation into IL-1Ra dynamics and its implications for FMF treatment in the future is recommended.
ARTHRITIS & RHEUMATOLOGY
(2021)
Review
Cell Biology
Shu Fang, Ditte Gry Ellman, Ditte Caroline Andersen
Summary: The current research on small-diameter tissue-engineered vascular grafts (SD-TEVGs) focuses on improving design to address issues like mechanical failure, thrombogenicity, and intimal hyperplasia that have limited success so far. Synthetic polymer-based SD-TEVGs have shown limited success in large-animal models, while natural polymer and cell-secreted ECM-based SD-TEVGs are still facing challenges in terms of strength and thrombogenicity. Further exploration of allogeneic native ECM-based SD-TEVGs, in vitro-developed hybrid SD-TEVGs with allogeneic banked human cells or isolated autologous stem cells, and in-body tissue architecture (IBTA)-based SD-TEVGs appear promising for the future due to their dimension, mechanical strength, biocompatibility, and availability.
Article
Biochemistry & Molecular Biology
Alexander Hogsted Ahlmann, Shu Fang, Sussi Bagge Mortensen, Line Weis Andersen, Pernille Gejl Pedersen, Johanne Juel Callesen, Sara Thornby Bak, Kate Lykke Lambertsen, Ditte Caroline Andersen
Summary: The study found that the decellularised umbilical artery graft did not trigger a significant immune response in mice and in an ex vivo allogeneic human setup, supporting its potential use as a tissue engineered vascular graft (TEVG).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ditte Gry Ellman, Ibrahim Mohamad Slaiman, Sabrina Bech Mathiesen, Kristian Skriver Andersen, Wolfgang Hofmeister, Elke Annette Ober, Ditte Caroline Andersen
Summary: Ischemic heart disease is a major cause of death globally, but zebrafish offer a unique model for studying heart regeneration due to their regenerative capacity. This paper visualizes and describes the apex resection procedure in detail, emphasizing the importance of anesthesia concentration and duration for balancing sedation levels and reducing mortality. Furthermore, examples of assessing zebrafish heart regeneration in both 2D and 3D are provided to assist beginners in establishing the AR model and encouraging further improvements and evaluations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Marlene Louise Quaade, Pratibha Dhumale, Simon Gabriel Comerma Steffensen, Hans Christian Beck, Eva Bang Harvald, Charlotte Harken Jensen, Lars Lund, Ditte Caroline Andersen, Soren Paludan Sheikh
Summary: The study found that diabetes does not appear to limit the effectiveness of autologous adipose stem cell therapy for treating erectile dysfunction (ED). Both diabetic and non-diabetic rats' adipose stem cells showed similar abilities in restoring erectile function and inducing genes related to endothelial function recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Orthopedics
Kira Stengaard, Eva Kildall Hejbol, Peter Toft Jensen, Matilda Degn, Thi My Linh Ta, Allan Stensballe, Ditte Caroline Andersen, Henrik Daa Schroder, Kate Lykke Lambertsen, Lars Henrik Frich
Summary: Rotator cuff tendon tear leads to impaired shoulder function and pain. This study investigated the biological response of the supraspinatus muscle to supraspinatus tendon tear. The findings demonstrate severe signs of acute inflammation, early degeneration, and fatty infiltration in the supraspinatus muscle, as well as reduced regenerative potential following tendon tear.
JOURNAL OF SHOULDER AND ELBOW SURGERY
(2022)
Article
Multidisciplinary Sciences
Mikkel O. Norgard, Lasse B. Steffensen, Didde R. Hansen, Ernst-Martin Fuchtbauer, Morten B. Engelund, Henrik Dimke, Ditte C. Andersen, Per Svenningsen
Summary: The study successfully created an EV reporter mouse model that allows for Cre-dependent labeling of extracellular vesicles (EVs), enabling the study of cell-specific EVs in vivo and gaining insights into their physiological and pathophysiological function.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Biomedical
Selgin D. Cakal, Carmen Radeke, Juan F. Alcala, Ditte G. Ellman, Sarkhan Butdayev, Ditte C. Andersen, Kirstine Calloe, Johan U. Lind
Summary: This study presents a simple and scalable method for generating physiologically relevant models of skeletal muscle using dual-material micro-extrusion. The approach allows for the generation of aligned, extended, and contractile myotubes in soft substrates, without the need for high-resolution patterning.
BIOMEDICAL MATERIALS
(2022)
Article
Rheumatology
S. B. Mortensen, A. E. Hansen, K-E Byg, L. Diederichsen, C. Schade Larsen, M. Goldschmidt, M. A. Jakobsen, K. Assing, K. A. Lambertsen, D. C. Andersen, I. S. Johansen
Summary: This study aims to identify potential discriminatory markers for familial Mediterranean fever (FMF) diagnosis. The secretion profile of pyrin inflammasome-activated monocytes in FMF patients and healthy donors was analyzed, and it was found that the levels of CCL1 and CXCL1 could differentiate FMF patients from healthy donors.
SCANDINAVIAN JOURNAL OF RHEUMATOLOGY
(2023)
Article
Biology
Minna Christiansen Lund, Ditte Gry Ellman, Maiken Nissen, Pernille Sveistrup Nielsen, Pernille Vinther Nielsen, Carina Jorgensen, Ditte Caroline Andersen, Han Gao, Roberta Brambilla, Matilda Degn, Bettina Hjelm Clausen, Kate Lykke Lambertsen
Summary: The neuroinflammatory response is a critical event in spinal cord injury, and tumor necrosis factor (TNF) plays a key role in this process. The expression of TNF and its receptors TNFR1 and TNFR2 were studied in mice after SCI, and it was found that TNF expression increased in both the acute and delayed phases. This study also found that diminishing detrimental immune signaling after SCI could enhance recovery in humans.
Article
Cardiac & Cardiovascular Systems
Sara Thornby Bak, Eva Bang Harvald, Ditte Gry Ellman, Sabrina Bech Mathiesen, Ting Chen, Shu Fang, Kristian Skriver Andersen, Christina Duhring Fenger, Mark Burton, Mads Thomassen, Ditte Caroline Andersen
Summary: While fetal heart CMs divide, postnatal CMs become binucleated or polyploid due to the lack of karyokinesis and/or cytokinesis, hindering heart regeneration. This study uses scRNA-seq to identify TFs involved in CM proliferation and terminal differentiation. By combining FACS and scRNA-seq, the researchers generate high-resolution transcriptomic maps of diploid and tetraploid CMs, revealing ZEB1 as a key regulator of CM proliferation and endoreplication.
BASIC RESEARCH IN CARDIOLOGY
(2023)
Article
Cell & Tissue Engineering
Shu Fang, Alexander Hogsted Ahlmann, Louise Langhorn, Kamal Hussein, Jens Ahm Sorensen, Xiaowei Guan, Soren Paludan Sheikh, Lars Peter Riber, Ditte Caroline Andersen
Summary: PCL scaffolds showed enhanced cytocompatibility with adipose regenerative cells in vitro, and in vivo experiments demonstrated higher patency rates for PCL tubes under antithrombotic treatment compared to control groups.
REGENERATIVE MEDICINE
(2021)
Article
Cell & Tissue Engineering
Lidan Zhang, Manami Kubota, Ayasa Nakamura, Takayuki Kaji, Shigeto Seno, Akiyoshi Uezumi, Ditte Caroline Andersen, Charlotte Harken Jensen, So-ichiro Fukada
Summary: The study demonstrates that abnormal expression of Dlk1 in CalcR-cKO MuSCs impairs quiescence, while the CalcR-PKA-Yap1 pathway inhibits Dlk1 expression in quiescent MuSCs. Loss of Dlk1 rescues the quiescent state in CalcR-cKO MuSCs.
