期刊
GROWTH FACTORS
卷 29, 期 6, 页码 271-277出版社
INFORMA HEALTHCARE
DOI: 10.3109/08977194.2011.607136
关键词
Granulosa cell; amphiregulin; heparin-binding EGF-like growth factor; vascular endothelial growth factor; follicular development
资金
- Ministry of Education, Science and Culture of Japan [22591829]
- Grants-in-Aid for Scientific Research [22591829] Funding Source: KAKEN
The function of granulosa cells is regulated by various hormones and growth factors. Our aim is to clarify the regulation of vascular endothelial growth factor (VEGF) production induced by heparin-binding EGF-like growth factor (HB-EGF) and amphiregulin (AR) in a human granulosa cell line, KGN. KGN cells were cultured and incubated for 24 h with HB-EGF and AR. The levels of VEGF in the culture media were measured by an enzyme-linked immunosorbent assay. The activation of MAP kinase in KGN cells was detected by Western blot analysis. VEGF production was significantly increased by HB-EGF or AR alone in a dose-dependent manner, whereas it was decreased by AG1478 or U0126. The MAP kinase activity was increased by treatment with HB-EGF or AR. The results suggested that VEGF is induced by HB-EGF and AR through mechanisms involving MAP kinase. The increase in VEGF may contribute to neovascularization, which in turn would promote various ovulation phenomena as well as follicular growth.
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