期刊
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
卷 246, 期 9, 页码 1249-1254出版社
SPRINGER
DOI: 10.1007/s00417-008-0872-x
关键词
DBA/2J mouse; Muller cell; reactive gliosis; retinal degeneration
Purpose To investigate the membrane physiology of Muller glial cells from retinae of DBA/2J mice (which develop ocular hypertension) and of C57BL/6 control mice of different ages. Methods Retinae were obtained at the ages of 3, 6, and 12 months from DBA/2J mice and from C57BL/6 controls. Immunohistochemistry was performed using antibodies against glial fibrillary acidic protein (GFAP). Whole-cell membrane currents, membrane potentials and capacitances were recorded from freshly isolated Muller cells. Results Strong immunostaining for GFAP was found in Muller cells of 12-month-old DBA/2J mice, whereas only astrocytes were immunopositive in C57BL/6 retinae. No significant alterations of membrane currents or potentials of Muller cells from DBA/2J mice as compared to controls were observed at any age; however, the membrane capacitance was increased in Muller cells from DBA/2J mice at the age of 6 months. Conclusions Although Muller cells of DBA/2J mice display some symptoms of reactive gliosis, the lack of significant alterations of the membrane physiology confirm previous data demonstrating that these cells undergo a nonproliferative gliosis.
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