期刊
GLYCOBIOLOGY
卷 24, 期 8, 页码 728-739出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwu036
关键词
human milk oligosaccharides; immunodeterminants; milk group classification
资金
- European Union/European Regional Development Fund
- Dutch Ministry of Economic Affairs, Agriculture and Innovation
- Dutch innovation program 'Peaks in the Delta'
- Municipality of Groningen
- Provinces of Groningen, Fryslan, and Drenthe
- Dutch Carbohydrate Competence Center (CCC) [WP6a]
Human milk oligosaccharides (HMOs) are a major constituent of human breast milk and play an important role in reducing the risk of infections in infants. The structures of these HMOs show similarities with blood group antigens in protein glycosylation, in particular in relation to fucosylation in Lewis blood group-type epitopes, matching the maternal pattern. Previously, based on the Secretor and Lewis blood group system, four milk groups have been defined, i.e. Lewis-positive Secretors, Lewis-positive non-Secretors, Lewis-negative Secretors and Lewis-negative non-Secretors. Here, a rapid one-dimensional H-1 nuclear magnetic resonance (NMR) analysis method is presented that identifies the presence/absence of (alpha 1-2)-, (alpha 1-3)- and (alpha 1-4)-linked fucose residues in HMO samples, affording the essential information to attribute different HMO samples to a specific milk group. The developed method is based on the NMR structural-reporter-group concept earlier established for glycoprotein glycans. Further evaluation of the data obtained from the analysis of 36 HMO samples shows that within each of the four milk groups the relative levels of the different fucosylation epitopes can greatly vary. The data also allow a separation of the Lewis-positive Secretor milk group into two sub-groups.
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