Article
Food Science & Technology
Teodora Dukic, Katarina Smiljanic, Jelena Mihailovic, Ivana Prodic, Danijela Apostolovic, Shu-Hua Liu, Michelle M. Epstein, Marianne van Hage, Dragana Stanic-Vucinic, Tanja Cirkovic Velickovic
Summary: This study investigates the impact of peanut roasting on the post-translational modification (PTM) profiles of major peanut allergens. It finds that roasting can induce changes in certain PTMs, and Ara h 1 is the most modified major allergen in both raw and roasted samples.
Review
Biochemistry & Molecular Biology
Tibo De Coninck, Koen Gistelinck, Henry C. Janse van Rensburg, Wim van den Ende, Els J. M. Van Damme
Summary: Plant development is an ongoing process involving morphological, (epi)genetic, and metabolic changes from pollination to seed maturation and germination. Glycosylation and sugar signaling play crucial roles in plant development, but there is still much to learn about their significance, importance, and interaction with stress responses.
Article
Biochemistry & Molecular Biology
Van-An Duong, Onyou Nam, EonSeon Jin, Jong-Moon Park, Hookeun Lee
Summary: Emiliania huxleyi, a cosmopolitan coccolithophore, was studied for the global discovery of post-translational modifications (PTMs) using a three-dimensional separation method combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methylation was found to be the most frequent PTM, with lysine being the most frequently modified amino acid. The number of identified proteins increased by 22.5% after the PTM search, with some proteins showing a significant increase in intensity.
Review
Biochemistry & Molecular Biology
Gengjun Zhu, Lifang Jin, Wanchun Sun, Shuang Wang, Ning Liu
Summary: This review highlights the importance of post-translational modifications (PTMs) in colorectal cancer (CRC) and discusses their role in diagnosis, prognosis, and treatment. The review summarizes the proteomic profiling strategies for key PTMs and describes the methods used to delineate the global landscapes of each PTM. The review also discusses the current status of PTM research in CRC and provides future perspectives on the role of PTM regulation in translational medicine for CRC.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Article
Immunology
Neil G. Rumachik, Stacy A. Malaker, Nicole K. Paulk
Summary: Advances in gene therapy product and process development have improved our understanding of the basic biology of recombinant AAV, with a particular focus on post-translational modifications of the capsid protein. Concerns about immunogenic responses to high doses of systemically administered rAAV have emphasized the importance of cataloging and tracking potentially immunogenic components of vector lots.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daniel Salas-Lloret, Roman Gonzalez-Prieto
Summary: Ubiquitination and SUMOylation are dynamic post-translational modifications that regulate cellular processes. The understanding of these modifications has been increased by the development of mass spectrometry-based approaches. This translated article provides a concise overview of the basic mechanisms of ubiquitination and SUMOylation, as well as recent MS-based approaches for specific target identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Juliane Hermann, Leon Schurgers, Vera Jankowski
Summary: Post-translational modifications play a key role in protein function and disease progression, but their reliable detection and quantification remain a challenge in clinical diagnosis. Mass spectrometry offers a solution, but its clinical translation still faces challenges.
MOLECULAR ASPECTS OF MEDICINE
(2022)
Article
Parasitology
Sebastian Miles, Javier Magnone, Joaquin Garcia-Luna, Sylvia Dematteis, Gustavo Mourglia-Ettlin
Summary: This study provides insights into the post-translational modifications (PTMs) of proteins in E. granulosus s.l., including the identification of new PTMs and the enzymes involved. These findings expand our knowledge on PTMs in platyhelminthes.
Article
Microbiology
Paul D. Veith, Mikio Shoji, Nichollas E. Scott, Eric C. Reynolds
Summary: Porphyromonas gingivalis, an important human pathogen and model organism, has been shown to have O-glycosylation. This study identified 257 putative glycosylation sites in 145 glycoproteins and revealed the structure of the major O-glycan. The O-glycosylation system in P. gingivalis plays a role in establishing the physicochemical properties of the periplasm.
MICROBIOLOGY SPECTRUM
(2022)
Review
Biochemistry & Molecular Biology
Ivana Samarzija
Summary: The specific functional form of a protein is mostly achieved through dynamic interactions of many enzymes in post-translational modifications, which cells widely use to respond to stimuli, regulate transcription, and maintain proteostasis. Post-translational modifications play important roles in driving prostate cancer, particularly in regulating protein activity at the level of post-translational modifications.
Article
Chemistry, Multidisciplinary
Yinping Tian, Qiang Zhu, Zeyu Sun, Didi Geng, Bingyi Lin, Xiaoling Su, Jiahui He, Miao Guo, Hong Xu, Ye Zhao, Weijie Qin, Peng George Wang, Liuqing Wen, Wen Yi
Summary: This study identifies a novel O-GlcNAcylated protein and reveals its regulatory role in cell cycle progression and DNA damage response.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemical Research Methods
Paige E. Lawler, James G. Bollinger, Suzanne E. Schindler, Cynthia R. Hodge, Nicolas J. Iglesias, Vishal Krishnan, John B. Coulton, Iran Li, David M. Holtzman, Randall J. Bateman
Summary: Although the APOE 4 allele is highly associated with sporadic Alzheimer's disease, the mechanism of apolipoprotein (apoE) and AD pathophysiology remains unclear. This study developed a LC-MS/MS assay to measure both unmodified and O-glycosylated apoE peptides in plasma and cerebrospinal fluid (CSF). The results indicate that plasma apoE glycosylation levels correlate with total apoE levels, APOE genotype, and amyloid status, suggesting it could be a marker of brain amyloidosis and may contribute to the pathophysiology of AD.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Microbiology
Paul D. D. Veith, Dhana G. G. Gorasia, Eric C. C. Reynolds
Summary: In this study, the O-glycoproteome of F. johnsoniae was characterized and 325 O-glycosylation sites were identified among 226 glycoproteins. The glycosylated domains were mainly located in the periplasm, which is believed to be beneficial for protein folding and stability. Additionally, six proteins associated with gliding motility or the T9SS were shown to be O-glycosylated.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Plant Sciences
Jonas Giese, Juergen Eirich, Dirk Walther, Youjun Zhang, Ines Lassowskat, Alisdair R. Fernie, Marlene Elsaesser, Veronica G. Maurino, Markus Schwarzlaender, Iris Finkemeier
Summary: The transition from dark to light in the diurnal cycle causes significant physiological changes in plant metabolism, which require specific modes of regulation. While the activities of key metabolic enzymes regulated by light-dependent post-translational modifications (PTMs) have been extensively studied at the protein level, a comprehensive understanding of the global dynamics of light-dependent PTMs is lacking. In this study, we investigated the changes in the metabolome and proteome in Arabidopsis rosettes in response to light in a time-dependent manner, focusing on phosphorylation, lysine acetylation, and cysteine-based redox switches. Our findings revealed that over 1700 out of more than 24,000 detected PTM sites were altered during the transition from dark to light, with different compartments showing distinct PTM changes at different timepoints.
Article
Chemistry, Analytical
Amanda Helms, Edwin E. E. Escobar, Saulius Vainauskas, Christopher H. H. Taron, Jennifer S. S. Brodbelt
Summary: The complete characterization of O-glycopeptides, including identification of peptides, localization of glycosites, and mapping of glycans, has been a challenging task in O-glycoproteomics. This study successfully applied a strategy combining the use of O-glycoprotease IMPa and HCD-triggered UVPD to achieve the complete characterization of O-glycopeptides. This approach allowed the localization of multiple adjacent or proximal O-glycosites and the identification of a previously unknown glycosite on etanercept.
ANALYTICAL CHEMISTRY
(2023)