期刊
GLYCOBIOLOGY
卷 21, 期 8, 页码 994-999出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwq198
关键词
Asn-X-Cys sequence; B subunit of Shiga toxin 2e; N-glycosylation; plant cells
资金
- Ministry of Economy, Trade and Industry of Japan
The vesicular transport pathway in plant cells is often used for higher accumulation of recombinant proteins. In the endoplasmic reticulum, which acts as a gateway to the vesicular transport pathway, N-glycosylation occurs on specific Asn residues. This N-glycosylation in recombinant proteins must be carefully regulated as it can impact their enzymatic activity, half lives in serum when injected, structural stability, etc. In eukaryotic cells, including plant cells, N-glycans were found to be attached to Asn residues in Asn-X-Ser/Thr (X not equal Pro) sequences. However, recently, N-glycosylations at noncanonical Asn-X-Cys sequences have been found in mammals and yeast. Our laboratory has discovered that N-glycans are attached to Asn residues at Asn-Thr-Cys sequences of double-repeated B subunit of Shiga toxin 2e produced in plant cells, the first reported case of N-glycosylation at a noncanonical Asn-X-Cys sequence in plant cells.
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