Article
Biochemical Research Methods
Jonathan L. Babulic, Chantelle J. Capicciotti
Summary: In this study, an exo-enzymatic strategy using recombinant human sialyltransferase ST6GAL1 and a diazirine-linked CMP-Neu5Ac derivative was developed to selectively introduce photo-cross-linking probes onto cell-surface glycoconjugates. This approach showed high efficiency and improved cross-linking compared to the current methods, and has the potential to interrogate and identify glycoconjugate ligands for diverse glycan-binding proteins.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Giulio Fittolani, Elena Shanina, Monica Guberman, Peter H. Seeberger, Christoph Rademacher, Martina Delbianco
Summary: This study presents a rapid approach that combines automated glycan assembly with high-throughput NMR methods for studying protein-glycan interactions. Through screening synthetic Lewis type 2 antigens, preferences for fucosylated glycans were confirmed, and previously unknown weak interactions between glycans and lectins were discovered.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Fisheries
Jhonatas Teixeira Viana, Rafael dos Santos Rocha, Rodrigo Maggioni
Summary: Lectins are important pattern recognition receptors in the immune system of L. vannamei, involved in multiple immune response pathways.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Review
Cell Biology
Franziska Krautter, Asif J. Iqbal
Summary: The recruitment of leukocytes is a complex process involving interactions between proteins expressed by the endothelium and circulating leukocytes. Recent research has expanded our understanding of these interactions, identifying novel lectins and regulatory pathways. Targeting glycans and glycan-binding proteins could offer new therapeutic strategies for modulating leukocyte recruitment and transmigration during inflammation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Analytical
Zachary Klamer, Brian Haab
Summary: MotifFinder is a novel software algorithm for glycan-array analysis that accurately interprets data from diverse datasets, enabling the identification of binding constants for distinct motifs and providing broader views of lectin specificity. This advancement promises to enhance studies on protein-glycan interactions.
ANALYTICAL CHEMISTRY
(2021)
Article
Biology
Sumnima Singh, Patricia Bastos-Amador, Jessica Ann Thompson, Mauro Truglio, Bahtiyar Yilmaz, Silvia Cardoso, Daniel Sobral, Miguel P. Soares
Summary: This study shows that deletion of the Ggta1 gene can shape the composition of gut microbiota via an Ig-dependent mechanism, where antibodies play a role in reducing microbiota pathogenicity. This suggests that loss of GGTA1 function in ancestral primates might have been influenced by microbiota interactions.
Review
Multidisciplinary Sciences
Hongliang Zhang, Jie Gao, Yujie Tang, Tengchuan Jin, Jinhui Tao
Summary: Inflammasomes play a crucial role in both the innate and adaptive immune systems, acting as a bridge between these two components to maintain a fine balance in immune responses. Understanding the function of inflammasomes in lymphocytes can provide new insights into the mechanisms and treatment of various diseases, including autoimmune diseases and tumors.
JOURNAL OF ADVANCED RESEARCH
(2023)
Review
Immunology
Rinaldo Rodrigues dos Passos Junior, Gisele Facholi Bomfim, Fernanda R. Giachini, Rita C. Tostes, Victor Vitorino Lima
Summary: The O-GlcNAcylation modification of proteins plays a significant role in cardiovascular diseases, including hypertension. Recent studies have shown that components of innate and adaptive immunity are targets for O-GlcNAcylation and may contribute to arterial hypertension.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yixuan Xie, Siyu Chen, Qiongyu Li, Ying Sheng, Michael Russelle Alvarez, Joeriggo Reyes, Gege Xu, Kemal Solakyildirim, Carlito B. Lebrilla
Summary: A new cross-linking mass spectrometry method has been developed to directly identify glycan-protein interactions on the cell membrane, providing a new approach to unravel glycomic interactions on cell membranes.
Article
Fisheries
Wei Wang, Mei-Yi Liu, Chen-Jie Fei, Chang-Hong Li, Jiong Chen
Summary: In this study, the ladderlectin-like sequence of ayu fish was cloned, showing its critical role in innate immunity and protective effects against bacterial infection, ultimately increasing survival rates.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Bailee N. Kain, Brandon T. Tran, Pamela N. Luna, Ruoqiong Cao, Duy T. Le, Marcus A. Florez, Laure Maneix, Jack D. Toups, Daniel E. Morales-Mantilla, Scott Koh, Hyojeong Han, Roman Jaksik, Yun Huang, Andre Catic, Chad A. Shaw, Katherine Y. King
Summary: Recent studies have found that infection can reprogram hematopoietic stem and progenitor cells (HSPCs) to enhance innate immune responses, a phenomenon known as trained immunity. In this study using mice, it was discovered that HSPCs activate interferon gamma-response genes heterogeneously upon primary infection, and rare cell populations expand. Macrophages derived from trained HSPCs demonstrated enhanced bacterial killing and metabolism, and a single dose of recombinant interferon gamma exposure was enough to induce similar training. Furthermore, transplantation of influenza-trained HSPCs in mice resulted in enhanced immunity against M. avium, demonstrating cross-protection against different pathogens. These findings suggest that HSPCs can produce long-term bone marrow derived macrophages with enhanced function and provide cross-protection against alternative pathogens.
Article
Chemistry, Multidisciplinary
Alvaro M. Martins, Tania M. Lopes, Francisca Diniz, Jose Pires, Hugo Osorio, Filipe Pinto, Daniela Freitas, Celso A. Reis
Summary: Alterations in glycosylation machinery in cancer cells lead to the synthesis of abnormal glycan structures in tumor cells. Extracellular vesicles (EVs) are involved in cancer communication and progression, and certain tumor-associated glycans have been identified in cancer EVs. This study investigates the impact of 3D tumor architecture on the selective packaging of cellular glycans into EVs. The results show differential packaging of specific proteins and glycans in EVs produced by 2D and 3D cultured cells, suggesting distinct biological functions and correlation with clinical data.
Article
Biology
Tsukushi Kamiya, Nicole M. Davis, Megan A. Greischar, David Schneider, Nicole Mideo
Summary: Our study revealed that high host mortality during acute malaria infection could be attributed to weak parasite clearance or inadvertent removal of uninfected cells in excess. Molecular immunology assays indicated that these two distinct mechanisms of poor survival might be due to low expression of certain inflammatory cytokines.
Article
Multidisciplinary Sciences
Daniel J. Honigfort, Meghan O. Altman, Pascal Gagneux, Kamil Godula
Summary: The study utilized synthetic glycopolymer mimetics to model the mucosal glycocalyx on red blood cells and evaluated its impact on lectin and virus adhesion. The results suggest that the glycocalyx inhibits adhesion of lectins and viruses in a size- and density-dependent manner, supporting the concept of mucins as protective shields against pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Editorial Material
Immunology
Xinxin Wang, Yuping Lai
Summary: In this study, the authors investigate the mechanism by which pattern recognition receptors NOD1 and NOD2 sense bacterial muropeptides to induce inflammation in keratinocytes. They demonstrate that solute carrier 46A2 (SLC46A2) transports DAP-muropeptides into the cytosol, leading to NOD1 activation and subsequent skin inflammation during psoriasis.
