Article
Biotechnology & Applied Microbiology
Ananya Nanda, Sourya Subhra Nasker, Anoop K. Kushwaha, Deepak Kumar Ojha, Albert K. Dearden, Saroj K. Nayak, Sasmita Nayak
Summary: Gold nanoparticles can significantly increase protein splicing efficiency and N-terminal cleavage efficiency, providing an effective strategy for engineering splicing-enhanced intein platforms.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Physical
David Teze, Joan Coines, Folmer Fredslund, Kshatresh D. Dubey, Gonzalo N. Bidart, Paul D. Adams, John E. Dueber, Birte Svensson, Carme Rovira, Ditte H. Welner
Summary: The study found that UGT1 from Polygonum tinctorium can catalyze N-, O-, or S-glycosylation with similar rates, and elucidated the molecular basis of N-, O-, and S-specificities using experimental mutagenesis and simulations. It also showed that the active site can be engineered to increase or favor one of the three glycosylation activities over another, leading to the design of more active and specific enzyme variants for glycoside production.
Article
Chemistry, Analytical
Miloslav Sanda, Lindsay Morrison, Radoslav Goldman
Summary: The outbreak of the Covid-19 pandemic has caused the current global health crisis, prompting the need for detailed studies on SARS-CoV-2 proteins to develop effective antiviral compounds and vaccines. The glycosylation of the spike (S) protein plays a significant role in its proteolytic activation, expanding the current knowledge in this area.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Michael L. Nosella, Maria Tereshchenko, Iva Pritisanac, P. Andrew Chong, Jeffrey A. Toretsky, Hyun O. Lee, Julie D. Forman-Kay
Summary: The study found that O-GlcNAcylation can reduce the phase separation tendency of the EWS protein, and knockdown of the OGT gene in cells decreases the O-GlcNAcylation of EWS, affecting its accumulation in biomolecular condensates. O-GlcNAcylation may play a general role in regulating biomolecular condensates.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Myung Jin Oh, Unyong Kim, Sol Kim, Dae Sik Cho, Jung-A Seo, Nari Seo, Joo An
Summary: Glycosylation plays a crucial role in the quality, stability, safety, immunogenicity, pharmacokinetics, and efficacy of biotherapeutics. Various methods have been used for glyco-characterization at different levels. Intact protein analysis is considered a rapid glycoform monitoring approach; however, it can be challenging for biotherapeutics with multiple glycosylation sites. A new strategy using two-step intact glycoform mass spectrometry has been developed to address this challenge, providing rapid and accurate information on the degree of glycosylation. This method can be used to assess glycosylation similarity between batches and between biosimilar and reference during development and production.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Xiaoyang Hou, Yu Wang, Dongbo Bu, Yaojun Wang, Shiwei Sun
Summary: N-linked glycosylation is a crucial protein modification involved in various biological processes. The EMNGly approach, which combines machine learning and deep learning, improves glycosylation site prediction accuracy and outperforms existing techniques.
Article
Biochemistry & Molecular Biology
Viridiana Garcia-Ruiz, Patricia Orduna, Antonia Castillo-Rodal, Teresa J. Flores-Rodriguez, Yolanda Lopez-Vidal
Summary: This study aimed to investigate the differences in glycosylation profiles between two recombinant strains of Mycobacterium microti and found distinct proteome and glycoproteome profiles between the strains.
Article
Multidisciplinary Sciences
Thanuja D. Sudasinghe, Michael T. Banco, Donald R. Ronning
Summary: EgtD is a crucial enzyme for EGT synthesis in M. tb, and inhibitors targeting this enzyme could potentially shorten Tuberculosis treatment regimens by weakening important bacterial defenses. The inhibitors identified bind in various regions of the enzyme active site, providing structural information for developing more potent inhibitors.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Jialin Liu, Bo Cheng, Xinqi Fan, Xinyue Zhou, Jiankun Wang, Wen Zhou, Hengyu Li, Wenfeng Zeng, Pengyuan Yang, Xing Chen
Summary: Proteins are extensively modified with glycans of different structures, making it challenging to map the protein glycosylation landscape. This study presents Click-iG, a method that enables the simultaneous enrichment and profiling of three types of intact glycopeptides using metabolic labeling of glycans, optimized mass spectrometry, and tailored software analysis. Click-iG was able to identify thousands of intact glycosites in cell lines and living mice, providing comprehensive coverage of the protein glycosylation landscape and laying the foundation for investigating crosstalk between different glycosylation pathways.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biology
Fabrizio Menardo, Caroline Colijn
Summary: Detecting factors associated with transmission is crucial for understanding disease epidemics and designing effective public health measures. In this study, the author used a simulation-based approach to investigate the influence of various epidemiological processes on clustering and TBL analyses of Mycobacterium tuberculosis genomic data sets. The results show that different factors, except the length of the infectious period, affect the outcomes of clustering and TBL distributions. The study also highlights the limitations of current interpretations of these analyses and suggests that optimal clustering thresholds and TBL distributions may vary depending on epidemiological conditions.
Article
Chemistry, Organic
Majid Ahmad Ganie, Faheem Fayaz, Muneer-ul-Shafi Bhat, Masood Ahmad Rizvi, Shabnam Raheem, Bhahwal Ali Shah
Summary: A mild approach for the synthesis of N-acyl-N,O-hemiacetals has been reported, utilizing an electron donor-acceptor complex. This protocol allows for direct access to electrophilic N-acylimines at room temperature without prefunctionalization of the hydroxyl group. The in situ generated N-acylimine reacts with different nucleophiles to yield diverse scaffolds.
Article
Biochemistry & Molecular Biology
Luz Diaz-Storani, Anaelle A. Clary, Diego M. Moreno, Maria Sol Ballari, Exequiel O. J. Porta, Andrea B. J. Bracca, Jonathan B. Johnston, Guillermo R. Labadie
Summary: A series of C15-C20 isoprenyl derivatives were synthesized as possible substrates of the methyl-branched lipid omega-hydroxylase CYP124A1 from Mycobacterium tuberculosis. These derivatives were characterized for their interaction with the enzyme active site using UV-vis spectroscopy. While some analogs showed similar binding affinity to parent substrates, they were not oxidized in the enzyme assay system. Molecular docking simulations revealed a binding mode where the unsaturated functional groups of the analogs were sequestered within the active site, providing insight into the lack of detectable turnover or inhibition.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Seonjeong Lee, Shinyeong Ju, Seok Jin Kim, Jin-Oh Choi, Kihyun Kim, Darae Kim, Eun-Seok Jeon, Cheolju Lee
Summary: tipNrich is an optimized N-terminal analysis method for analyzing small amounts of protein samples, allowing for rapid assessment of protein quality or characterization of N-terminal proteoform-related diseases.
ANALYTICAL CHEMISTRY
(2021)
Review
Plant Sciences
Richard Strasser, Georg Seifert, Monika S. Doblin, Kim L. Johnson, Colin Ruprecht, Fabian Pfrengle, Antony Bacic, Jose M. Estevez
Summary: Glycosylation is a crucial modification process that can alter the biological function of proteins and lipids, affecting the development and physiology of an organism. While challenges remain in understanding plant glycosylation, high throughput approaches may help to address these gaps, revolutionizing the identification and characterization of unknown plant glycosyltransferases and hydrolases.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Chemistry, Medicinal
Cheng-Hsin Chiu, Janet Jia-Yin Tan, Soumik Mondal, Chun-Hung Lin, Kwok-Kong Tony Mong
Summary: This up-to-date review focuses on the chemical synthesis and immunological studies of Mtb SGLs and structural analogues, highlighting the development of new glycosylation methods and the asymmetric synthesis of polymethylated scaffolds. Both methods are critical for advancing further research on the biological functions of these complex SGLs.
Article
Chemistry, Medicinal
Bryna L. Fitzgerald, M. Nurul Islam, Barbara Graham, Sebabrata Mahapatra, Kristofor Webb, W. Henry Boom, Stephanus T. Malherbe, Moses L. Joloba, John L. Johnson, Jill Winter, Gerhard Walzl, John T. Belisle
ACS INFECTIOUS DISEASES
(2019)
Article
Infectious Diseases
Carlos A. M. Silva, Barbara Graham, Kristofor Webb, Laura Vari Ashton, Marisa Harton, Annie F. Luetkemeyer, Samantha Bokatzian, Reem Almubarak, Sebabrata Mahapatra, Laura Hovind, Michelle A. Kendall, Diane Havlir, John T. Belisle, Mary Ann De Groote
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2019)
Article
Biotechnology & Applied Microbiology
Diego A. Espinosa, P. Robert Beatty, Henry Puerta-Guardo, M. Nurul Islam, John T. Belisle, Rushika Perera, Eva Harris
JOURNAL OF GENERAL VIROLOGY
(2019)
Article
Immunology
Katharina Ronacher, Novel N. Chegou, Lanie Kleynhans, Joel F. Djoba Siawaya, Nelita du Plessis, Andre G. Loxton, Elizna Maasdorp, Gerard Tromp, Martin Kidd, Kim Stanley, Magdalena Kriel, Angela Menezes, Andrea Gutschmidt, Gian D. van der Spuy, Robin M. Warren, Reynaldo Dietze, Alphonse Okwera, Bonnie Thiel, John T. Belisle, Jacqueline M. Cliff, W. Henry Boom, John L. Johnson, Paul D. van Helden, Hazel M. Dockrell, Gerhard Walzl
Article
Multidisciplinary Sciences
Emma K. Harris, Marisa R. Harton, Maria Angela de Mello Marques, John T. Belisle, Claudia R. Molins, Nicole Breuner, Gary P. Wormser, Robert D. Gilmore
SCIENTIFIC REPORTS
(2019)
Article
Biochemical Research Methods
Bryna L. Fitzgerald, Claudia R. Molins, M. Nurul Islam, Barbara Graham, Petronella R. Hove, Gary P. Wormser, Linden Hu, Laura Ashton, John T. Belisle
JOURNAL OF PROTEOME RESEARCH
(2020)
Article
Immunology
Bryna L. Fitzgerald, Barbara Graham, Mark J. Delorey, Adoracion Pegalajar-Jurado, M. Nurul Islam, Gary P. Wormser, John N. Aucott, Alison W. Rebman, Mark J. Soloski, John T. Belisle, Claudia R. Molins
Summary: This study used metabolomics to analyze metabolic responses in patients with Post-treatment Lyme disease symptoms/syndrome (PTLDS) compared to clinically cured non-PTLDS patients. The findings revealed observable metabolic differences between PTLDS and non-PTLDS patients at multiple time points, with distinct patterns of metabolite abundance. A small number of metabolites could be used to define PTLDS versus non-PTLDS patients at specific time points, and these findings were validated in a second patient cohort. These results suggest that metabolite-based measurement can help distinguish patients with PTLDS and provide insights into the underlying biochemistry of PTLDS.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Biology
Cassandra Koh, M. Nurul Islam, Yixin H. Ye, Nunya Chotiwan, Barbara Graham, John T. Belisle, Konstantinos A. Kouremenos, Saravanan Dayalan, Dedreia L. Tull, Stephan Klatt, Rushika Perera, Elizabeth A. McGraw
COMMUNICATIONS BIOLOGY
(2020)
Article
Microbiology
Gabriela Balderrama-Gutierrez, Ana Milovic, Vanessa J. Cook, M. Nurul Islam, Youwen Zhang, Hippokratis Kiaris, John T. Belisle, Ali Mortazavi, Alan G. Barbour
Summary: The study revealed significant differences in responses to infection between white-footed deermice and mice, suggesting that deermice possess adaptive traits to moderate inflammation and better balance infection resistance and tolerance. These findings have implications for studying infection tolerance in other important reservoir species.
Article
Immunology
Matthew K. Muramatsu, Jianli Zhou, Bryna L. Fitzgerald, Ranjit K. Deka, John T. Belisle, Michael Norgard
Summary: This research highlights the importance of riboflavin and Flavin pools for the growth of Borrelia burgdorferi and suggests that flavin utilization and its downstream products may play a more significant role in the pathogenesis of this spirochete than previously thought. Mutation in specific genes related to riboflavin import and synthesis can lead to resistance to certain antimicrobial agents and attenuation of disease in a murine model.
INFECTION AND IMMUNITY
(2021)
Article
Multidisciplinary Sciences
Petronella R. Hove, Forgivemore Magunda, Maria Angela de Mello Marques, M. Nurul Islam, Marisa R. Harton, Mary Jackson, John T. Belisle
Summary: This study identified the key enzyme BB0572 responsible for cholesterol glycolipid formation in Bb, demonstrating its function as a galactosyltransferase. Mutation in BB0572's predicted active site hindered its ability to catalyze the formation of cholesterol glycolipids.
Article
Immunology
Thabatta L. S. A. Rosa, Maria Angela M. Marques, Zachary DeBoard, Kelly Hutchins, Carlos Adriano A. Silva, Christine R. Montague, Tianao Yuan, Julio J. Amaral, Georgia C. Atella, Patricia S. Rosa, Katherine A. Mattos, Brian C. VanderVen, Ramanuj Lahiri, Nicole S. Sampson, Patrick J. Brennan, John T. Belisle, Maria Cristina Pessolani, Marcia Berredo-Pinho
Summary: Upon infection, Mycobacterium leprae induces accumulation of cholesterol in Schwann cells, which is potentially delivered to the intracellular bacterium. Cholesterol oxidation is an essential catabolic pathway for M. leprae pathogenicity, with 3 beta-HSD as a potential drug target for leprosy treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Eric R. Kehoe, Bryna L. Fitzgerald, Barbara Graham, M. Nurul Islam, Kartikay Sharma, Gary P. Wormser, John T. Belisle, Michael J. Kirby
Summary: The study developed a pipeline for data preprocessing, biomarker selection, and classification of LCMS serum samples for Lyme disease diagnosis, achieving a 98.13% test balanced success rate. The methodology is generalizable and can be adapted to other LCMS or metabolomics data sets.
SCIENTIFIC REPORTS
(2022)
Correction
Immunology
Thabatta L. S. A. Rosa, Maria Angela M. Marques, Zachary DeBoard, Kelly Hutchins, Carlos Adriano A. Silva, Christine R. Montague, Tianao Yuan, Julio J. Amaral, Georgia C. Atella, Patricia S. Rosa, Katherine A. Mattos, Brian C. VanderVen, Ramanuj Lahiri, Nicole S. Sampson, Patrick J. Brennan, John T. Belisle, Maria Cristina V. Pessolani, Marcia Berredo-Pinho
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Nastassja L. Kriel, Mae Newton-Foot, Owen T. Bennion, Bree B. Aldridge, Carolina Mehaffy, John T. Belisle, Gerhard Walzl, Robin M. Warren, Samantha L. Sampson, Nico C. Gey van Pittius
Summary: In this study, the sub-cellular location of EccA(3) in mycobacteria was investigated. Time-lapse microscopy showed that EccA(3) localizes to the growing pole in M. smegmatis. The co-purification of EccA(3) with proteins involved in polar growth, mycolic acid synthesis, and secretion systems suggests that EccA(3) is located at the site of active cell growth.