Article
Cell Biology
Michael Bachmann, Artiom Skripka, Kai Weissenbruch, Bernhard Wehrle-Haller, Martin Bastmeyer
Summary: Integrin-mediated adhesions serve as convergence points for multiple signaling pathways. By using super-resolution microscopy, we discovered spatially defined clusters of pPax and pFAK within focal adhesions, providing new insights into the organization and functions of these adhesion structures.
JOURNAL OF CELL SCIENCE
(2022)
Article
Cell Biology
Ioanna Antoniades, Maria Kyriakou, Anna Charalambous, Katerina Kalalidou, Andri Christodoulou, Maria Christoforou, Paris A. Skourides
Summary: The study successfully prevented the action of FAK at focal adhesions by generating a specific peptide structure, thereby effectively inhibiting the migration and invasion of tumor cells and providing a new direction for the development of anti-metastatic agents.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Cell Biology
Nan-Peng Chen, Jonas Aretz, Reinhard Faessler
Summary: The CDK1-cyclin B1 complex phosphorylates kindlin to induce its degradation, leading to the disassembly of focal adhesions and cell rounding at mitotic entry.
NATURE CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Karin Legerstee, Tsion E. Abraham, Wiggert A. van Cappellen, Alex L. Nigg, Johan A. Slotman, Adriaan B. Houtsmuller
Summary: Focal adhesions (FAs) are flat elongated structures consisting of three layers along the vertical axis. Different proteins extend at different parts of the FAs, and their redistribution may be involved in cell movement during migration processes.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Xu Lu, Huijun Liu, Zixuan Cai, Zhichao Hu, Minxiu Ye, Yue Gu, Yue Wang, Dan Wang, Qun Lu, Zhongxia Shen, Xinhua Shen, Chao Huang
Summary: Depressed mice have lower numbers of microglia in the dentate gyrus. Reversing this decline with a single low dose of lipopolysaccharide has antidepressant effects. The antidepressant effect is related to the synthesis and signaling pathway of brain-derived neurotrophic factor.
BRAIN BEHAVIOR AND IMMUNITY
(2022)
Article
Biochemistry & Molecular Biology
Eleni Michael, Savvas Polydorides, Vasilis J. Promponas, Paris Skourides, Georgios Archontis
Summary: Novel approaches for inhibiting FAK and PYK2 involve interfering with protein interactions promoting localization at FAs, validated by techniques such as surface plasmon resonance and fluorescence polarization assays. Molecular dynamics simulations provide insights into the recognition of peptides with LD motifs by FAK-FAT and PYK2-FAT domains, aiding in the design of inhibitory peptides and pharmacophore models for potential small-molecule inhibitors.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2021)
Article
Biology
Marta Ripamonti, Andrea Lamarca, Norman E. Davey, Diletta Tonoli, Sara Surini, Ivan de Curtis
Summary: Scaffold protein liprin-alpha 1 plays an important role in assembling plasma membrane-associated platforms (PMAPs) at the front of migrating breast cancer cells. It interacts with B56 regulatory subunits and mediates the interaction between liprin-alpha 1 and the heterotrimeric PP2A holoenzyme. B56 gamma/PP2A is a novel component of the PMAPs machinery regulating tumor cell motility.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qianqian He, Siu Kwan Sze, Kai Soon Ng, Cheng-Gee Koh
Summary: The composition of focal adhesions (FAs) in mouse embryonic stem cells (mESCs) is similar to that of differentiated cells, but the maturation of FAs is less in mESCs. The combination of different quantitative proteomics methods allows for a comprehensive and targeted analysis of the molecular composition of FAs in mESCs.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Xiaoquan Li, Joseph Dale Combs, Khalid Salaita, Xiaokun Shu
Summary: Researchers have developed a genetically encoded FAK activity sensor called SPARK, which allows visualization of endogenous FAK activity in living cells and vertebrates. They have discovered polarized FAK activity at the distal tip of newly formed single focal adhesions (FAs) in migrating cells, and found that FAK activity is proportional to the strength of tension applied to FAs. These findings advance our understanding of the mechanistic processes involved in cell migration.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Neurosciences
Mingxie Ni, Meng Zheng, Bingran Chen, Xu Lu, Hui Zhao, Tao Zhu, Li Cheng, Han Han, Ting Ye, Huijun Liu, Ying Ye, Chao Huang, Xiaomei Yuan
Summary: Intranasal administration of low-dose lipopolysaccharide (LPS) is shown to improve depression-like behavior in chronically stressed mice through the stimulation of hippocampal microglia. The antidepressant effects are BDNF-dependent and mediated by the ERK1/2 signaling pathway. Inhibition or depletion of microglia can abolish the antidepressant effects of LPS.
Article
Pharmacology & Pharmacy
Bingran Chen, Cheng Zhao, Haojie Zhu, Xu Lu, Huijun Liu, Qun Lu, Tao Zhu, Chao Huang
Summary: 8-Glucan exhibits antidepressant effects by stimulating microglia-mediated activation of ERK1/2 and synthesis of BDNF in the hippocampus.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Richard M. Lucas, Lin Luo, Jennifer L. Stow
Summary: This article summarizes the important role of ERK1/2 in cell signaling, particularly in immune responses in immune cells. Additionally, it discusses the dysfunctional signaling of ERK1/2 in inflammatory diseases and explores the potential therapeutic benefits of targeting this pathway in inflammation.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Article
Pharmacology & Pharmacy
Wei Xu, Frank Bearoff, Sandhya Kortagere
Summary: This study demonstrates that biased signaling agonists of D3R have differential effects on ERK1/2, which may be advantageous for the development of better drugs.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Fisheries
Junjie Wan, Xuelin Zhao, Jiqing Liu, Kaiyu Chen, Chenghua Li
Summary: In this study, a novel SFK homologue (AjSrc) was identified in Apostichopus japonicus, which mediated Vibrio splendidus-induced coelomocytes' phagocytosis via interacting with focal adhesion kinase (AjFAK) and co-phosphorylation. This study enriched the mechanism of phagocytosis in echinoderm and provided a new theoretical foundation for disease control of sea cucumber.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Yangyang Zhou, Siying Wang, Wei Wu, Jing Ling, Haoyu Li, Qi Jia, Jiaojiao Zheng, Xingling Zheng, Ruobing Yu, Qiangxin Wu, Yaoping Shi, Cor Lieftink, Roderick L. Beijersbergen, Shengxian Yuan, Rene Bernards, Haojie Jin, Wenxin Qin
Summary: This study identified mitochondrial translation-related genes associated with proliferation in liver cancer cells. Tigecycline disrupted mitochondrial translation process and induced respiratory chain deficiency, showing therapeutic potential for liver cancer. MEK inhibitors were identified as synergistic drugs to Tigecycline-insensitive liver cancer cells. Blockade of EGFR-ERK1/2-MYC cascade combined with Tigecycline could be a potential therapeutic strategy for liver cancer.
Article
Biochemistry & Molecular Biology
Yu-Seon Kang, Eun-Jeong Jeong, Hyun-Jeong Seok, Seon-Kyu Kim, Jin-Seong Hwang, Mu Lim Choi, Dong-Gyu Jo, Yuna Kim, Jinhyeon Choi, Yeo-Jin Lee, Eunsun Jung, Jeong-Ki Min, Tae-Su Han, Jang-Seong Kim
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Pharmacology & Pharmacy
Young-Lai Cho, Jong-Gil Park, Hyo Jin Kang, Wooil Kim, Min Ji Cho, Ju-Hong Jang, Min-Gi Kwon, Sungsik Kim, Sang-Hyun Lee, Jangwook Lee, Yeon-Gu Kim, Young-Jun Park, Won Kon Kim, Kwang-Hee Bae, Byoung-Mog Kwon, Sang J. Chung, Jeong-Ki Min
PHARMACOLOGICAL RESEARCH
(2019)
Article
Biotechnology & Applied Microbiology
Kyungmin Lee, Jangwook Lee, Minjeong Kwak, Young-Lai Cho, Byungtae Hwang, Min Ji Cho, Na Geum Lee, Jongjin Park, Sang-Hyun Lee, Jong-Gil Park, Yeon-Gu Kim, Jang-Seong Kim, Tae-Su Han, Hyun-Soo Cho, Young-Jun Park, Seon-Jin Lee, Hee Gu Lee, Won Kon Kim, In Cheul Jeung, Nam Woong Song, Kwang-Hee Bae, Jeong-Ki Min
JOURNAL OF NANOBIOTECHNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Min Ji Cho, Sung-Jin Yoon, Wooil Kim, Jongjin Park, Jangwook Lee, Jong-Gil Park, Young-Lai Cho, Jeong Hun Kim, Hyejin Jang, Young-Jun Park, Sang-Hyun Lee, Jeong-Ki Min
EXPERIMENTAL AND MOLECULAR MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Na Geum Lee, In Cheul Jeung, Soon Chul Heo, Jinhoi Song, Wooil Kim, Byungtae Hwang, Min-Gi Kwon, Yeon-Gu Kim, Jangwook Lee, Jong-Gil Park, Min-Gyeong Shin, Young-Lai Cho, Mi-Young Son, Kwang-Hee Bae, Sang-Hyun Lee, Jae Ho Kim, Jeong-Ki Min
Article
Cell Biology
Seung-Ho Park, Sung-Jin Yoon, Song Choi, Jun-Seob Kim, Moo-Seung Lee, Seon-Jin Lee, Sang-Hyun Lee, Jeong-Ki Min, Mi-Young Son, Choong-Min Ryu, Jiyun Yoo, Young-Jun Park
CELL DEATH & DISEASE
(2020)
Article
Engineering, Biomedical
Jongjin Park, Na Geum Lee, Mihee Oh, Jinhoi Song, Wooil Kim, Min-Gi Kwon, Seul Gi Kim, Baek Soo Han, Kwang-Hee Bae, Dong Gwang Lee, Sang-Hyun Lee, Jong-Gil Park, Jae Ho Kim, Jangwook Lee, Jeong-Ki Min
Article
Neurosciences
Xiaoye Feng, Yang Fan, Chang Y. Chung
Article
Biochemistry & Molecular Biology
Sang-Hyun Lee, Jin-Man Kim, Dong Gwang Lee, Jangwook Lee, Jong-Gil Park, Tae-Su Han, Hyun-Soo Cho, Young-Lai Cho, Kwang-Hee Bae, Young-Jun Park, Seon-Jin Lee, Moo-Seung Lee, Yong-Min Huh, Deog Yeon Jo, Hwan-Jung Yun, Heung Jin Jeon, Nayoung Kim, Mina Joo, Jang-Seong Kim, Hyo Jin Lee, Jeong-Ki Min
Summary: The reduction of Dsg2 in gallbladder carcinoma is associated with poor prognosis, treatment resistance, and disease progression. Inhibiting Src activity could enhance therapeutic response to anti-EGFR therapy and improve clinical efficacy.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Oncology
Byul Moon, Suk-Jin Yang, Seong Min Park, Sang-Hyun Lee, Kyu Sang Song, Eun-Jeong Jeong, Mijin Park, Jang-Seong Kim, Young Il Yeom, Jung-Ae Kim
Article
Immunology
Ben Bassett, Selvaraj Subramaniyam, Yang Fan, Seth Varney, Hope Pan, Ana M. D. Carneiro, Chang Y. Chung
Summary: Minocycline shows promising results in alleviating depressive-like behaviors induced by chronic mild stress, potentially by directly acting on microglia to reduce their phagocytic potential and reverse the pathogenic phagocytic potential of neurotoxic M1 microglia. This mechanism may help reduce negative phenotypes associated with reduced neurogenesis caused by exposure to chronic stress.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Oncology
Kyungmin Lee, Sang-Hyun Lee, Wooil Kim, Jangwook Lee, Jong-Gil Park, Jang-Seong Kim, Jung Tae Kim, Yea Eun Kang, Minho Shong, Hyo Jin Lee, Jin-Man Kim, Won Gu Kim, Bon Seok Koo, Koon Soon Kim, Jeong-Ki Min
ENDOCRINE-RELATED CANCER
(2020)