Article
Cardiac & Cardiovascular Systems
Jiro Ikeda, Corey A. Scipione, Sharon J. Hyduk, Marwan G. Althagafi, Jawairia Atif, Sarah A. Dick, Maneesha Rajora, Erika Jang, Takuo Emoto, Junichi Murakami, Noriko Ikeda, Hisham M. Ibrahim, Chanele K. Polenz, Xiaotang Gao, Kelly Tai, Jenny Jongstra-Bilen, Ryota Nakashima, Slava Epelman, Clinton S. Robbins, Gang Zheng, Warren L. Lee, Sonya A. MacParland, Myron Cybulsky
Summary: This study revealed that radiation exposure inhibits LDL entry into the aortic intima at baseline and the earliest stages of atherosclerosis in a mouse model of bone marrow transplantation. Single-cell transcriptomic analysis showed a redirection of LDL uptake by endothelial cells to lysosomal degradation and reverse cholesterol transport pathways, reducing lipid accumulation within the intima and impacting lesion initiation and growth.
CIRCULATION RESEARCH
(2021)
Article
Immunology
Gregory P. Takacs, Christian J. Kreiger, Defang Luo, Guimei Tian, Julia S. Garcia, Loic P. Deleyrolle, Duane A. Mitchell, Jeffrey K. Harrison
Summary: Glioblastoma (GBM) is a highly malignant brain tumor with poor survival rates. The tumor microenvironment (TME) of GBM is characterized by immunosuppressive cells, including CCR2(+)/CX3CR1(+) myeloid-derived suppressor cells (MDSCs). These MDSCs are recruited to the TME through the chemokines CCL2 and CCL7. Targeting CCL2 and CCL7 may be a promising strategy for GBM treatment.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Hematology
Hannah Kinoshita, Mamatha Mandava, Mariah Jensen-Wachspress, Haili Lang, Elisabeth Joy, Jay Tanna, Chase D. McCann, Samuel O'Brien, Sianna Burnett, Abeer Shibli, Fahmida Hoq, Monica Bhatia, Patrick J. Hanley, Blachy Davila Saldana, Kris M. Mahadeo, Catherine M. Bollard, Michael D. Keller, Allistair Abraham
Summary: Hematopoietic stem cell transplantation (HSCT) is increasingly used to treat sickle cell disease (SCD). However, this treatment increases the risk of viral infection. This study explores the use of virus-specific T-cell (VST) therapies after HSCT to prevent or treat viral infections in SCD patients.
Article
Biochemistry & Molecular Biology
Adrien Guerard, Victor Laurent, Gaelle Fromont, David Esteve, Julia Gilhodes, Edith Bonnelye, Sophie Le Gonidec, Philippe Valet, Bernard Malavaud, Nicolas Reina, Camille Attane, Catherine Muller
Summary: Bone metastasis is a common and deadly complication of prostate cancer, and adipocyte-rich bone marrow cells may contribute to the migration of cancer cells through the release of chemokines. Factors such as obesity and aging can exacerbate the aggressiveness of tumors and may require evaluation of the CCR3/CCL7 axis for potential pharmacological interventions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Shixiang Zhou, Yaqiong Li, Lexin He, Min Chen, Weihong Li, Ting Xiao, Jian Guan, Zhenhua Qi, Qi Wang, Siyuan Li, Pingkun Zhou, Zhidong Wang
Summary: This study suggests that haptoglobin can be used as both an early molecular marker of radiation injury and an important indicator for bone marrow transplantation therapy for radiation injury.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Medicine, General & Internal
Alice Bertaina, Paul C. Grimm, Kenneth Weinberg, Robertson Parkman, Karen M. Kristovich, Giulia Barbarito, Elizabeth Lippner, Girija Dhamdhere, Vasavi Ramachandran, Jordan M. Spatz, Sahar Fathallah-Shaykh, T. Prescott Atkinson, Amira Al-Uzri, Geraldine Aubert, Kim van der Elst, Sean G. Green, Rajni Agarwal, Priscila F. Slepicka, Ami J. Shah, Maria G. Roncarolo, Amy Gallo, Waldo Concepcion, David B. Lewis
Summary: This study aimed to find a new approach to eliminate immune rejection and the need for post-transplantation immunosuppression. By performing bone marrow and kidney transplantation on three patients with a specific genetic condition, the researchers successfully achieved the goal of normal renal function without the need for immunosuppression.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Hematology
Changzheng Li, Binghuo Wu, Yishan Li, Yaxi Liu, Jin Wang, Jiayi Xie, Xi Xu, Xiaobin Tian, Zhitao Ye, Jingjing Guan, Jie Chen, Siyu Xie, Baolin Zhang, Boyong Cai, Qianhao Wang, Haopeng Yu, Tian Lan, Cheuk Him Man, Xunlei Kang, Pengxu Qian, John M. Perry, Aibin He, Linjia Jiang, Meng Zhao
Summary: This study shows that deleting the enzyme Sphk2 enhances the self-renewal and regenerative potential of HSCs, improves their function, and sustains their multilineage potential during aging. Mechanistically, Sphk2 deletion stabilizes HIF1a protein to increase hypoxic responses and improve the metabolic fitness of HSCs.
Article
Clinical Neurology
Antonio Uccelli, Alice Laroni, Rehiana Ali, Mario Alberto Battaglia, Morten Blinkenberg, Lou Brundin, Michel Clanet, Oscar Fernandez, James Marriot, Paolo Muraro, Seyed Massood Nabavi, Roberto S. Oliveri, Ernst Radue, Cristina Ramo Tello, Irene Schiavetti, Johann Sellner, Per Soelberg Sorensen, Maria Pia Sormani, Jens Thomas Wuerfel, Mark S. Freedman
Summary: The MESEMS study aimed to evaluate the safety and activity of autologous bone marrow-derived MSCs in patients with active multiple sclerosis. The results showed that MSC treatment did not affect GELs and does not support its use in treating multiple sclerosis.
Article
Microbiology
Shiela McCollam, James S. Lewis, Joseph Bubalo, Amber Diaz
Summary: This study aimed to evaluate the efficacy and safety of 300-mg once-monthly intravenous (IV) pentamidine prophylaxis in 702 adult allogeneic hematopoietic stem cell transplant (HSCT) patients. The results showed that IV pentamidine administration effectively prevented Pneumocystis jirovecii pneumonia (PJP), but breakthrough Nocardia and Toxoplasma infections were observed in a certain percentage of patients.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biology
Kazuki Inoue, Yongli Qin, Yuhan Xia, Jie Han, Ruoxi Yuan, Jun Sun, Ren Xu, Jean X. Jiang, Matthew B. Greenblatt, Baohong Zhao
Summary: Adipoq-lineage progenitors in bone marrow are the main cellular source of M-CSF, producing much higher levels than osteoblast lineage cells. Deficiency of M-CSF in these progenitors significantly reduces the generation of bone marrow macrophages and osteoclasts, leading to severe osteopetrosis.
Editorial Material
Hematology
Susan E. Prockop
Summary: In this article, the authors demonstrate that a standardized treatment approach combined with novel composite end points can establish a standard of care for boys with this rare nonmalignant disorder even in the absence of prospective trials.
Article
Multidisciplinary Sciences
Laura Breda, Tyler E. Papp, Michael P. Triebwasser, Amir Yadegari, Megan T. Fedorky, Naoto Tanaka, Osheiza Abdulmalik, Giulia Pavani, Yongping Wang, Stephan A. Grupp, Stella T. Chou, Houping Ni, Barbara L. Mui, Ying K. Tam, Drew Weissman, Stefano Rivella, Hamideh Parhiz
Summary: This study developed a nanoparticle system that can target hematopoietic stem cells in vivo and achieved correction of diseased cells through mRNA delivery. Furthermore, this system enables non-genotoxic conditioning for stem cells, providing a new approach for HSCT.
Editorial Material
Hematology
Mark Leick, Yi-Bin Chen
Summary: In this study, McCurdy et al compare immune restoration after haploidentical and matched donor HCT, and find that high-dose PTCy-based GVHD prophylaxis is effective in immune recovery.
Review
Immunology
Hanli Xu, Shuye Lin, Ziyun Zhou, Duoduo Li, Xiting Zhang, Muhan Yu, Ruoyi Zhao, Yiheng Wang, Junru Qian, Xinyi Li, Bohan Li, Chuhan Wei, Keqiang Chen, Teizo Yoshimura, Ji Ming Wang, Jiaqiang Huang
Summary: Over the past thirty years, the importance of chemokines and their receptors has been recognized in various immune processes and disease pathogenesis. The genetic and nongenetic regulation of chemokines and receptors leads to functional heterogeneity and their imbalances contribute to various diseases. Understanding the molecular basis and interactions of chemokines and receptors will advance the application of precision medicine.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Imranul Alam, Rita L. Gerard-O'Riley, Dena Acton, Sara L. Hardman, Madeline Murphy, Marta B. Alvarez, Rachel J. Blosser, Anthony Sinn, Edward F. Srour, Melissa A. Kacena, Michael J. Econs
Summary: This study investigates the efficacy of bone marrow transplantation (BMT) for the treatment of autosomal dominant osteopetrosis type II (ADO2). The results show that BMT can restore osteoclast function and improve bone phenotypes in ADO2 mice. BMT is effective in both young and adult ADO2 mice.
Article
Biochemistry & Molecular Biology
Kevin Harkin, Josy Augustine, Alan W. Stitt, Heping Xu, Mei Chen
Summary: This study found that Wedelolactone (WD) can protect against N-methyl-N-nitrosourea (NMU) induced retinal neurodegeneration by suppressing the inflammasome-related pathway. The results showed that WD can attenuate the impairment of retinal function and structure caused by NMU and reduce the expression of inflammasome-related genes.
Article
Immunology
Caijiao Yi, Jian Liu, Wen Deng, Chang Luo, Jinyan Qi, Mei Chen, Heping Xu
Summary: The study demonstrated that MMP12 plays a critical role in the development of subretinal fibrosis, partially through promoting macrophage-to-myofibroblast transition. The findings suggest the potential therapeutic target of MMP12 in the treatment of end-stage complications of neovascular age-related macular degeneration.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Biochemistry & Molecular Biology
Blessing C. Ilochonwu, Marko Mihajlovic, Roel F. Maas-Bakker, Charis Rousou, Miao Tang, Mei Chen, Wim E. Hennink, Tina Vermonden
Summary: This study aims to develop a degradable in situ forming hydrogel that can be injected into the vitreous cavity for sustained release of therapeutic antibodies to treat retinal diseases. Through systematic experiments and analysis, the most suitable hydrogel formulation was identified and its effectiveness and bioactivity in vivo were demonstrated.
Article
Immunology
Maria Llorian-Salvador, Eimear M. Byrne, Manon Szczepan, Karis Little, Mei Chen, Heping Xu
Summary: The study found that complement activation plays a crucial role in the development of macular fibrosis, partially through the C5a-C5aR-mediated epithelial-to-mesenchymal transition (EMT). Therefore, targeting complement activation rather than C5a may be a novel approach for the management of macular fibrosis.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Urology & Nephrology
Sudha Priya Soundara Pandi, Michael J. Shattock, Bruce M. Hendry, Claire C. Sharpe
Summary: The role of different isoforms of TTCC in mouse mesangial cells was investigated in this study to determine the best therapeutic target for treating mesangioproliferative kidney diseases. Single and double knockout clones of the TTCC isoforms Ca(V)3.1 and Ca(V)3.2 were generated using CRISPR-cas9 gene editing. It was found that the Ca(V)3.1 isoform is required for stimulated phosphorylation of ERK1/2, while the Ca(V)3.2 isoform is not. Neither isoform was necessary for cell proliferation, and the anti-proliferative effects of mibefradil and TH1177 were not isoform-specific.
Review
Neurosciences
Manon Szczepan, Maria Llorian-Salvador, Mei Chen, Heping Xu
Summary: The process of subretinal wound healing is important, and when damage exceeds the healing capacity of the retinal defense system, circulating immune cells are involved, leading to abnormal wound healing and fibrosis.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jinyan Qi, Wei Pan, Ting Peng, Ling Zeng, Xiaoning Li, Zhongping Chen, Zhikuan Yang, Heping Xu
Summary: This retrospective study investigated the changes in circulating immune cells in patients with myopic retinopathy. The study used blood test results and demographic and ocular information from 392 myopic patients and 129 emmetropia controls. Compared to emmetropia, myopic patients had higher percentages of neutrophils and basophils and a higher neutrophil/lymphocyte ratio, while they had lower percentages of monocytes and lymphocytes, and lower counts of lymphocytes and eosinophils. The difference remained after adjusting for age and hypertension/diabetes. Additionally, platelet counts were significantly lower in myopic patients after adjustments. Further subgroup analysis showed that different levels of immune cells were related to different forms of myopic retinopathy, such as peripheral retinal degeneration and choroidal neovascularization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Gideon Obasanmi, Noemi Lois, David Armstrong, Jose M. Romero Hombrebueno, Aisling Lynch, Mei Chen, Heping Xu
Summary: This study investigated alterations in inflammatory cytokines in peripheral blood mononuclear cells (PBMCs) in people with type 1 diabetes (T1D) and different stages of diabetic retinopathy (DR). The results showed that in mild non-proliferative DR (mNPDR), IL-10 secretion was increased, while IL-6 and IFN-gamma expression were reduced. In active and inactive proliferative DR (aPDR), IL-6 and IL-10 secretion decreased, and IL-6 expression in CD11b(+) PBMCs increased. These findings suggest that IL-10, especially myeloid-produced IL-17A, may be involved in early stages of DR, and IL-6-producing myeloid cells may play a role in PDR development.
Article
Immunology
Caijiao Yi, Jian Liu, Wen Deng, Chang Luo, Jinyan Qi, Mei Chen, Heping Xu
Summary: This study investigated the effect of ageing on subretinal fibrosis secondary to choroidal neovascularization and the mechanism of action. The results showed that ageing increased the number of circulating fibrocytes and the profibrotic potential of BM-derived macrophages. Additionally, aged mice had significantly more severe subretinal fibrosis compared to young mice. The transplantation of BM from aged mice significantly increased subretinal fibrosis in young mice.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Cell Biology
Josy Augustine, Sofia Pavlou, Kevin Harkin, Alan W. Stitt, Heping Xu, Mei Chen
Summary: Diabetic retinopathy (DR) leads to visual impairment and blindness due to dysfunction and inflammation of Mu'' ller cells. Interleukin-33 (IL-33) plays a crucial immunomodulatory role in DR, and its absence promotes inflammation, neurodegeneration, and decreased neurotrophin secretion.
DISEASE MODELS & MECHANISMS
(2023)
Article
Ophthalmology
Gemma McIlwaine, Lajos Csincsik, Rachel Coey, Luping Wang, Denise Fitzgerald, Jill Moffat, Adam M. Dubis, Gavin McDonnell, Stella Hughes, Tunde Peto, Imre Lengyel
Summary: This study examined outer retinal changes in patients with multiple sclerosis (MS). Results showed thinning of the inner retina and thickening of the outer retina in patients with a history of optic neuritis. No changes were observed in patients without optic neuritis. Furthermore, cone density was found to be decreased in all MS cases.
OPHTHALMOLOGY SCIENCE
(2023)