期刊
GLIA
卷 57, 期 8, 页码 828-834出版社
WILEY-BLACKWELL
DOI: 10.1002/glia.20809
关键词
Ca2+/calmodulin-dependent protein kinase; Ca2+ imaging; Ca2+ waves; cyclic AMP; okadaic acid; protein phosphatase
资金
- NIH [R01NS048095, T32GM08181]
- DOD [W81XWH-04-1-0176]
- NIH/NICHD [K12 HD43489]
Astrocytes participate in signaling via Ca2+ transients that spread from cell to cell across a multicellular syncytium. The effect, if any, of these Ca2+ waves on the transcription of Ca2+/cAMP-regulatory element binding protein (CREB)dependent genes is not known. We report here that, unlike neurons, increasing intracellular Ca2+ in cultured mouse cortical astrocytes failed to activate CREB-dependent transcription, even though CREB was phosphorylated at serine 133. In contrast, both CREB phosphorylation and CREB-dependent transcription were robustly stimulated by increasing cAMP. The failure of Ca2+-activated transcription in astrocytes was correlated with the absence of CaMKIV, a Ca2+-dependent protein kinase required for Ca2+-stimulated gene transcription in neurons. The inability of Ca2+ to signal via CaMKIV may insulate CREB-dependent gene transcription in astrocytes from activation by Ca2+ waves. (C) 2008 Wiley-Liss, Inc.
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