期刊
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
卷 16, 期 7, 页码 580-585出版社
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1400234
关键词
Pure total flavonoid compounds; Human myeloid leukemia cells; Growth inhibition; Synergistic effect; Apoptosis
类别
资金
- Zhejiang Provincial Natural Science Foundation of China [LY14H080003, LY13H290011]
To investigate the potential effects of pure total flavonoid compounds (PTFCs) from Citrus paradisi Macfadyen separately or combined with arsenic trioxide on the proliferation of human myeloid leukemia cells and the mechanisms underlying the action of PTFCs. The effects of PTFCs separately or combined with arsenic trioxide on the proliferation and apoptosis of leukemia cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MU), fluorescence microscopy, and flow cytometry. Their effects on the expression levels Of apoptosis-related regulators were determined by Western blot assay. PTFCs combined with arsenic trioxide significantly inhibited the growth of Kasumi-1 cells, and apoptosis was confirmed by flow cytometry analysis. Hoechst 33258 staining showed more significant morphological changes and more apoptosis following the combined treatment. Western blots showed changes in the expression of genes for poly ADP-ribose polymerase (PARP), caspase 3/9, and P65. The results indicated that PTFCs separately or combined with arsenic trioxide inhibited proliferation of leukemia cells in vitro and induced their apoptosis by modulating the expression of apoptosis-related regulator genes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据