期刊
GEOMICROBIOLOGY JOURNAL
卷 31, 期 7, 页码 632-641出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/01490451.2014.884195
关键词
Bacillus mucilaginosus; Ca nutrition acquisition; carbonic anhydrase; geomicrobiology; heterologous expression; RT-qPCR
资金
- National Natural Science Foundation of China [41373078, 41173091]
- National Key Basic Research Program of China [2013CB956702]
Molecular mechanisms and gene regulation are of interest in the area of geomicrobiology in which the interaction between microbes and minerals is studied. This paper focuses on the regulation of the expression of carbonic anhydrase (CA) genes in Bacillus mucilaginosus and the effects of the expression product of the B. mucilaginosus CA gene in Escherichia coli on calcite weathering. Real-time fluorescent quantitative PCR (RT-qPCR) was used to explore the relationship between CA gene expression in B. mucilaginosus and promotion of calcite dissolution under condition of Ca2+ deficiency. The results showed that adding calcite to the medium, which lacks Ca2+, can up-regulate the expression of the bacterial CA genes to accelerate calcite dissolution for bacterial growth. CA genes from B. mucilaginosus were transferred into E. coli by cloning. We then employed crude enzyme extract from the resultant E. coli strain in calcite dissolution experiments. The enzyme extract promoted calcite dissolution. These findings provide direct evidence for the role of microbial CA on mineral weathering and mineral nutrition release.
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