期刊
GEOMICROBIOLOGY JOURNAL
卷 26, 期 4, 页码 256-263出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/01490450902892373
关键词
actinobacteria; diversity; hot springs
资金
- New Century Excellent Talents in University [NECT-05-0821]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
Actinobacterial diversity was investigated in three hot springs [Tengchong Frog Mouth (TFM) hot spring (in Tengchong, China), Kamchatka Robb Flag hot spring (in Kamchatka, Russia), and Nevada Boiling Spring (in Nevada, USA)], a Tengchong Frog Mouth hot spring-derived pond and a soil sample near the TFM hot spring. Culture-independent techniques were employed including denaturing gradient gel electrophoresis and actinobacterial 16S rRNA gene phylogenetic analysis. The three hot springs and the hot spring-derived pond had pH of 6.8 to 7.9 and temperature of 50 to 81C. Actinobacteria were for the first time found to be very diverse in hot springs with temperature up to 81C, indicating that they have an unexpected capability of adapting to hot spring environments. A total of 346 actinobacterial clones were obtained from the three hot springs, and these clones were identified as 31 RFLP types, falling into the Actinomycetales (only limit to the Frankineae suborder), the Rubrobacterales, uncultured Candidatus Microthrix, unclassified Actinobacteria, and uncultured Candidate Division OP10. Our data have implications for a better understanding of the distribution of Actinobacteria in hot springs.
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