期刊
GENOMICS
卷 104, 期 6, 页码 587-593出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2014.09.002
关键词
Cotton; Centromere; Retrotransposon; Tandem repeat
资金
- New Century Excellent Talents in University [NCET-10-0496]
- National Natural Science Foundation of China [31071460]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Centromere usually contains high-copy-number retrotransposons and satellite repeats, which are difficult to map, clone and sequence. Currently, very little is known about the centromere in cotton. Here, we sequenced a bacterial artificial chromosome (BAC) mapping to the centromeric region and predicted four long-terminal-repeat (LTR) retrotransposons. They were located in the heterochromatic centromeric regions of all 52 pachytene chromosomes in Gossypiumhirsutum. Fiber-FISH mapping revealed that these retrotransposons span an area of at least 1.8 Mb in the centromeric region. Comparative analysis showed that these retrotransposons generated similar, strong fluorescent signals in the D progenitor Gossypium raimondii but not in the A progenitor Gossypium herbaceum, suggesting that the centromere sequence of tetraploid cotton might be derived from the D progenitor. Centromeric regions were anchored on 13 chromosomes of D-genome sequence. Characterization of these centromere-related repeats and regions will enhance cotton centromere mapping, sequencing and evolutionary studies. (C) 2014 Elsevier Inc. All rights reserved.
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