期刊
GENOMICS
卷 103, 期 1, 页码 76-82出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2013.06.006
关键词
Novel intronic miRNA; CA repeat; TDP-43; NRXN1
资金
- National Key Basic Research and Development Program 973 [2009CB825401]
- Chinese Academy of Science Strategic Project of Leading Science and Technology [XDA01020402]
- Chinese Academy of Science Knowledge Innovation Project [KSCX2-EW-R-01-02]
- Innovation Program of Beijing Institute of Life Science [2010-Biols-CAS-0302]
The Tar DNA-binding protein 43 (TARDBP, TDP-43) regulates RNA processing and miRNA biogenesis and is known to be involved in neurodegeneration. Messenger RNA (mRNA) targets of TDP-43 have recently been systematically identified, but small RNAs (sRNAs) bound by TDP-43 have not been studied in details. Here, we reexamine cross-linking, immunoprecipitation and sequencing (CLIP-seq) data, and identify pre-miRNAs, miRNAs and piRNAs bound by TDP-43 in human and mouse brains. Subsequent analysis of TDP-43 binding miRNAs suggests that target genes are enriched in functions involving synaptic activities. We further identify a novel miRNA (miR-NID1) processed from the intron 5 of human neurexin 1, NRXN1, and show that miR-NID1 represses NRXN1 expression by binding to TDP-43. Our results are in accordance with previously published data indicating TDP-43 through binding of specific miRNAs to play roles in neurodevelopmental activities and neurological disorders and further our understanding of TDP-43 function. (C) 2013 The Authors. Published by Elsevier Inc. All rights reserved.
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