期刊
GENOMICS
卷 102, 期 5-6, 页码 431-441出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2013.09.005
关键词
High-throughput RNA sequencing; Erythropoiesis; Cell differentiation; Development; Gene regulatory networks
资金
- Chinese Academy of Sciences, Stem Cell and Regenerative Medicine Research [XDA01040405]
- National Key Scientific Instrument and Equipment Development Projects of China [2011YQ03013404]
- National Basic Research Program (973 Program) [2006CB910403]
- National Natural Science Foundation of China [31371300, 31100924]
- National Institute of Health grants of United States [DK077864]
To explore the mechanisms controlling erythroid differentiation and development, we analyzed the genome-wide transcription dynamics occurring during the differentiation of human embryonic stem cells (HESCs) into the erythroid lineage and development of embryonic to adult erythropoiesis using high throughput sequencing technology. HESCs and erythroid cells at three developmental stages: ESER (embryonic), FLER (fetal), and PBER (adult) were analyzed. Our findings revealed that the number of expressed genes decreased during differentiation, whereas the total expression intensity increased. At each of the three transitions (HESCs-ESERs, ESERs-FLERs, and FLERs-PBERs), many differentially expressed genes were observed, which were involved in maintaining pluripotency, early erythroid specification, rapid cell growth, and cell-cell adhesion and interaction. We also discovered dynamic networks and their central nodes in each transition. Our study provides a fundamental basis for further investigation of erythroid differentiation and development, and has implications in using ESERs for transfusion product in clinical settings. (C) 2013 Elsevier Inc. All rights reserved.
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