期刊
GENOMICS
卷 100, 期 6, 页码 363-369出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2012.08.002
关键词
Zebra finch; RNA-seq; Song learning; Gene expression; Illumina; Dosage compensation; Bird; Sex chromosome
资金
- NIH NIGMS [1RC1GM091556]
- BBSRC [BBS/E/D/20310000] Funding Source: UKRI
- MRC [MR/K001744/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/D/20310000] Funding Source: researchfish
- Medical Research Council [MR/K001744/1] Funding Source: researchfish
The derivation of stably cultured cell lines has been critical to the advance of molecular biology. We profiled gene expression in the first two generally available cell lines derived from the zebra finch. Using Illumina RNA-seq, we generated similar to 93 million reads and mapped the majority to the recently assembled zebra finch genome. Expression of most Ensembl-annotated genes was detected, but over half of the mapped reads aligned outside annotated genes. The male-derived G266 line expressed Z-linked genes at a higher level than did the female-derived ZFTMA line, indicating persistence in culture of the distinctive lack of avian sex chromosome dosage compensation. Although these cell lines were not derived from neural tissue. many neurobiologically relevant genes were expressed, although typically at lower levels than in a reference sample from auditory forebrain. These cell lines recapitulate fundamental songbird biology and will be useful for future studies of songbird gene regulation and function. (C) 2012 Elsevier Inc. All rights reserved.
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