Article
Microbiology
Fayna Diaz-San Segundo, Gisselle N. Medina, Edward Spinard, Anna Kloc, Elizabeth Ramirez-Medina, Paul Azzinaro, Steffen Mueller, Elizabeth Rieder, Teresa de los Santos
Summary: Foot-and-mouth disease is a significant viral disease affecting livestock, with live attenuated vaccines identified as one of the best options for early and long-lasting protection.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Virology
Kun Li, Yong He, Li Wang, Pinghua Li, Sheng Wang, Pu Sun, Huifang Bao, Yimei Cao, Xuerong Liu, Guoqiang Zhu, Yali Song, Xingwen Bai, Xueqing Ma, Yuanfang Fu, Hong Yuan, Jing Zhang, Jian Wang, Yingli Chen, Dong Li, Zhiyong Lou, Zaixin Liu, Zengjun Lu
Summary: Foot-and-mouth disease virus (FMDV) is a highly contagious virus that infects cloven-hoofed animals. In this study, researchers identified two cross-protective antigen sites on VP2 and VP3 using bovine-derived broadly neutralizing monoclonal antibodies. One site on VP3 was recognized by bnAb C4, while the other site on VP2 was recognized by 9 different bnAbs.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Chen Li, Jiabao Shi, Haiwei Wang, Efrain E. Rivera-Serrano, Decheng Yang, Guohui Zhou, Chao Sun, Craig E. Cameron, Li Yu
Summary: This study demonstrates the importance of FMDV genetic diversity in viral virulence and transmissibility in susceptible animals. The generation of a high-fidelity FMDV variant with a single amino acid substitution in polymerase highlights the impact of viral population diversity on interhost viral spread. Furthermore, the high genetic diversity of type O FMDV may contribute to its dominance in the global epidemic by increasing both virulence and transmissibility.
JOURNAL OF VIROLOGY
(2021)
Article
Pharmacology & Pharmacy
Syahru Agung Setiawan, Winston Zhenhao Liu, Pei-Wei Weng, Chia-Hwa Lee, Vijesh Kumar Yadav, Mardiah Suci Hardianti, Tsu-Yi Chao, Chi-Tai Yeh
Summary: In this study, the synergistic effects of the second generation BTK inhibitor zanubrutinib and the BCL-2 inhibitor navitoclax in suppressing Double-hit lymphoma (DHL) were demonstrated. Comprehensive in silico analysis from single-cell to bulk-level profiling validated the combination's ability to induce apoptosis and ferroptosis, effectively inhibiting DHL cell proliferation. Mechanistically, the combination disrupted NRF2 and HMOX1 while deactivating GPX4, triggering ferroptosis. In vivo studies showed that the combination of zanubrutinib and navitoclax effectively suppressed tumor growth.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Microbiology
Yimei Cao, Kun Li, Xiangchuan Xing, Guoqiang Zhu, Yuanfang Fu, Huifang Bao, Xingwen Bai, Pu Sun, Pinghua Li, Jing Zhang, Xueqing Ma, Jian Wang, Zhixun Zhao, Dong Li, Zaixin Liu, Zengjun Lu
Summary: In this study, a bovine broadly neutralizing monoclonal antibody (W145) against FMDV serotype A was successfully produced using fluorescence-based single-B-cell antibody technology. A competitive enzyme-linked immunosorbent assay (NAC-ELISA) was developed for the detection of neutralizing antibodies against FMDV serotype A. The NAC-ELISA showed high specificity and sensitivity and could be used to evaluate protective immunity.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Microbiology
Gyeongmin Lee, Hyo Rin Kang, Aro Kim, Jong-Hyeon Park, Min Ja Lee, Su-Mi Kim
Summary: This study found that quercetin has antiviral effects against FMDV by inducing interferon production and enhancing the immune system in animals. Therefore, quercetin could potentially be used as a novel and effective antiviral agent for controlling FMDV infection. However, further research on its application in livestock is needed.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Yan Xiao, Suling Zhang, He Yan, Xiaolin Geng, Yanwei Wang, Xin Xu, Mengyue Wang, Haohao Zhang, Baicheng Huang, Wenqiang Pang, Ming Yang, Kegong Tian
Summary: The study successfully expressed capsid proteins of FMDV serotype O in Escherichia coli with an optimal tandem permutation (VP0-VP3-VP1), showing a protein yield close to 1:1:1. Immunization of pigs with FMDV VLPs induced FMDV-specific immune responses effectively and dose-dependently. The stable FMDV VLPs with multiple epitope exposure were effective for the induction of an immune response in pigs, laying a foundation for further development of the FMDV subunit vaccine.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Infectious Diseases
Glen Guyver-Fletcher, Erin E. Gorsich, Michael J. Tildesley
Summary: Foot-and-mouth disease (FMD) is a deadly and economically impactful disease in livestock, still prevalent in certain regions of Asia and Africa. The controversy of whether carrier livestock can infect susceptible animals remains, but theoretically carrier transmission can support the persistence of FMD in endemic regions.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Veterinary Sciences
Geoff Jones, Cord Heuer, Wes Johnson, Douglas Begg, Andrew McFadden, Ashish Sutar, Ronello Abila, Clare Browning, Ginette Wilsden, Anna B. Ludi, Syseng Khounsy, Supatsak Subharat
Summary: To control foot-and-mouth disease through vaccination, it is important to have sufficient population coverage and high vaccine efficacy in the field. Post-vaccination surveys can be conducted to monitor vaccine coverage and performance. Understanding the performance of serological tests is crucial for accurate interpretation of data.
PREVENTIVE VETERINARY MEDICINE
(2023)
Article
Microbiology
Saad Bin Dajem, Kareem Morsy, Mohammed Alshehri, Attalla El-Kott, Essam Ibrahim, Haitham El-Mekkawy, Rewaida Abdel-Gaber
Summary: In this study, pentastomids parasitizing Berber skinks in the well-vegetated wadis of Jizan, Saudi Arabia were morphologically described and taxonomically analyzed using molecular techniques. The parasites were identified as sharp-tipped posterior-hook Raillietiella spp. with similarities to other pentastomid genera in GenBank. Phylogenetic analysis supported the inclusion of the parasites within the monophyletic Pentastomida clade.
REVISTA ARGENTINA DE MICROBIOLOGIA
(2021)
Article
Biochemistry & Molecular Biology
Ayman Saber Mohamed, Saad Bin Dajem, Mohammed Al-Kahtani, Sara Bayoumi Ali, Essam Ibrahim, Kareem Morsy, Sohair Ramadan Fahmy
Summary: This study utilized freshwater bivalves as indicators for the effects of silver/chitosan nanocomposites in the freshwater environment. Exposure to Ag-CS NCs caused oxidative stress responses and histological changes in the organs of Coelatura aegyptiaca, indicating its sensitivity to nanoparticle pollution.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2021)
Article
Biology
Mohammad Y. Alshahrani, Essam H. Ibrahim, Mohammed Asiri, Mona Kilany, Ahmad Alshehri, Ali G. Alkhathami, Kareem Morsy, Harish C. Chandramoorthy
Summary: The study found that the ethanolic extract of Coccinia indica exhibited inhibitory effects on MRSA, Candida auris, and Streptococcus pyogenes, while showing weaker effects on E. coli and Trichophyton rubrum. The aqueous extract did not show any inhibitory effects. The results demonstrate concentration-dependent antibacterial and antifungal activities of the plant extract, suggesting its potential in combating multidrug resistance.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Environmental Sciences
Ali S. Alshehri, Attalla F. El-Kott, Ayman E. El-Kenawy, Mohamed Samir A. Zaki, Kareem Morsy, Reham A. Ghanem, Eman T. Salem, Eman R. Ebealy, Heba S. Khalifa, Ahmed E. Altyar, Hussah I. M. AlGwaiz, Essam H. Ibrahim, Mohammed S. Mahmoud, Mohammad A. Dallak, Eman M. Abd-Ella
Summary: This study demonstrates that kaempferol has a nephroprotective effect against cadmium-induced nephropathy by reducing kidney damage, improving kidney function, and attenuating oxidative stress and inflammation. It achieves these effects by suppressing NF-kappa B p65 and activating Nrf2.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Multidisciplinary Sciences
Essam H. Ibrahim, Mohammad Y. Alshahrani, Hamed A. Ghramh, Hani Alothaid, Mona Kilany, Kareem Morsy, Attalla F. El-kott, Ramadan Taha, Haitham El-Mekkawy, Gamal A. EL-Shaboury, Ahmed A. El-Mansi, Mohammed E. A. Mohammed, Mahmoud A. Sayed, Ibrahim S. Yahia
Summary: The study demonstrates that Origanum majorana leaf acetone extract has anti-colorectal cancer effects by inducing cell division arrest and apoptosis in HT-29 human colon cancer cells.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Badria M. AL-Shehri, Reham M. Mashat, Rahaf M. H. Alshareef, Wed Mohammed Ali Alaerjani, Khalid Ali Khan, Hamed A. Ghramh, Essam H. Ibrahim, Majed A. Bajaber, Abdulaziz A. Zarbah, Mohammed Elimam Ahamed Mohammed
Summary: This study investigated the presence of artificial sweeteners in honey samples from Asir region-Saudi Arabia and measured their concentration along with glucose oxidase (GOX) activity and other quality parameters. The results showed that Acacia and Zizphus honey samples contained high amounts of sorbitol and trace levels of xylitol. The floral origin and year of harvest significantly influenced the pH, electric conductivity, and acidity of the honey samples.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Materials Science, Multidisciplinary
F. El-Sayed, Mai S. A. Hussien, T. H. AlAbdulaal, Abdel-Haleem Abdel-Aty, H. Y. Zahran, I. S. Yahia, Mohamed Sh. Abdel-wahab, Essam H. Ibrahim, Medhat A. Ibrahim, Hanan Elhaes
Summary: Cationic dye (methylene blue) and anionic dye (carmine) degradation were demonstrated using SrO and G-SrO NPs. The efficacy of photocatalysis and electro & photo-electrocatalytic oxidation in degrading carmine dye was compared. The study found that graphene-modified SrO nanoparticles showed improved photocatalytic activity and the 0.25G-SrO catalyst achieved high degradation efficiency for methylene blue dye under UV light.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2022)
Article
Multidisciplinary Sciences
Mohammad Y. Alshahrani, Essam H. Ibrahim, Mohammed Asiri, Mona Kilany, Ali G. Alkhathami, Mohammed N. Alshahrani, Harish C. Chandramoorthy
Summary: The addition of lycopene to the extract enhances the antioxidant and antibacterial activities, showing potential inhibition against multidrug-resistant bacteria.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Veterinary Sciences
K. Morsy, S. B. Dajem, A. Alghamdi, A. El-kott, E. Ibrahim, K. Attia, A. Al-Doaiss, H. El-Mekkawy, N. Sheraba, A. Baiomy, M. Fahmy, F. Shalaby
Summary: In 2020, 52 marine fish specimens were caught from boat landing sites along the Alexandria coast in Egypt. Among them, 30 fish were found to be infected with three different parasitic species, which can negatively impact fish health and lead to consumers rejecting the fish.
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
(2023)
Article
Parasitology
Kareem Morsy, Saad Bin Dajem, Mohammed Al-Kahtani, Attalla El-Kott, Essam Ibrahim, Hamida Hamdi, Amin Al-Doaiss, Mohamed Abumandour, Haitham El-Mekkawy, Diaa Massoud, Asmaa Adel, Shams Abd El-Kareem
Summary: Members of the order Trypanorhyncha are cestode parasites that infect the muscles of marine fish, affecting fish health and consumer behavior. This study identified four different species of parasites and confirmed their taxonomic position through molecular analysis. The results emphasize the importance of removing parasites during the commercialization of fish.
REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA
(2022)
Article
Pharmacology & Pharmacy
Essam Hassan Ibrahim, Mohammad Yahya Alshahrani, Hamed Ali Ghramh, Hani Alothaid, Mona Kilany, Kareem Morsy, Ramadan Taha, Khalid Mushabab Al Syaad, Haitham Ibrahim El-Mekkawy, Gamal Awad EL-Shaboury, Ahmed Abdel Aziz El-Mansi, Ahmad Alamri, Mahmoud Ahmed Sayed, Ibrahim Sayed Yahia, Rahaf Mohammed Hussein Alshareef, Badria Mohammed Al-Shehri, Mohammed Elimam Ahamed Mohammed
Summary: This study investigated the biological effects of Moringa oleifera leaf extract (MOE) alone or in combination with silver nanoparticles (AgNPs) on colon cancer and microbial cell growth. The results showed that MOE had mild antibacterial effects and increased apoptosis and p53 protein expression in colon cancer cells. MOE also arrested cell cycle at G2/M phase and stimulated splenic cell growth. Both extract preparations exhibited antioxidant activities. Thus, MOE can be utilized as an anticancer agent.
PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Environmental Sciences
Hamed A. Ghramh, Rahmah N. Al-Qthanin, Zubair Ahmad, Khalid Ali Khan, Essam H. Ibrahim, Habeeb Mansour A. Al-Solami, Ahlam Khalofah, Abeer Alahmari, Farhat S. Khan, Asma N. Asiri, Sally Negm, Mogbel Ahmed Abdalla El-Niweri, Fawziah M. Asiri
Summary: This study investigated the larvicidal toxicity of silver nanoparticles synthesized from the ethanol extract of S. palmeri against 4th instar larvae of Ae aegypti. The results showed that the silver nanoparticles were more effective in killing mosquitoes compared to the original extract.
FRESENIUS ENVIRONMENTAL BULLETIN
(2022)
Article
Zoology
Essam H. Ibrahim, Attalla F. El-kott, Ali Alshehri, Mona Kilany, Reza Yavari, Salahud Din, Diaa Massoud
Summary: The morphology and histology of the tongue in two adult Asian bears were examined using light and scanning electron microscopy, revealing four types of papillae. The tongue was covered by a keratinized stratified squamous epithelium, with a dense connective tissue making up the lamina propria and thick masses of striated muscles forming the bulk of the tongue. Lingual salivary glands with seromucous secretions were also present in the lingual root.
PAKISTAN JOURNAL OF ZOOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Essam H. Ibrahim, Ali Alshehri, Hamed A. Ghramh, Mona Kilany, Kareem Morsy, Attalla F. El-kott, Mahmoud A. Sayed
Summary: The study investigated the potential of Rosmarinus officinalis acetone extract and silver nanoparticles to target colorectal cancer cells without affecting normal cells. Results showed that the combination increased antibacterial activity, induced p53 expression, promoted apoptosis, and arrested cancer cells at a specific cell cycle phase, demonstrating promising effects on cancer treatment.
SCIENCE OF ADVANCED MATERIALS
(2021)
Article
Environmental Sciences
Khalid Ali Khan, Hamed A. Ghramh, Essam H. Ibrahim, Sadeq K. Alhag, Zubair Ahmad, Mona Kilany, Hussnain Ahmed Janjua, Habeeb Mansour A. Al-Solami
Summary: The biosynthesis of silver nanoparticles using vegetal extracts, particularly from Cinnamomum camphora leaves, shows larvicidal and antibacterial effects, along with some impact on normal cells and liver toxicity, though the liver function markers did not show significant changes after treatment with the silver nanoparticles.
FRESENIUS ENVIRONMENTAL BULLETIN
(2021)