Article
Biochemistry & Molecular Biology
Guanglu Sun, Zihao Yu, Qianwen Li, Yuanxing Zhang, Mingxiao Wang, Yunhui Liu, Jinze Liu, Lei Liu, Xuping Yu
Summary: Overexpression of the flhDC genes in Escherichia coli can lead to cell death due to excessive production of reactive oxygen species (ROS) from the oxidative phosphorylation pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Yan Zhang, Ran Wen, Jialu Bao, Yinglan Gong, Xiaodan Wang
Summary: This study analyzed the mechanism of mancozeb-induced kidney injury by detecting kidney function indicators, combined with transcriptome and metabolome sequencing. The results showed that mancozeb impaired the kidney structure, increased creatinine and uric acid levels, and accumulated reactive oxygen species in the kidneys. The differentially expressed genes were enriched in the oxidative phosphorylation pathway. The metabolomic analysis identified the cGMP-PKG signaling pathway as the main altered pathway in mancozeb-induced nephrotoxicity.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biotechnology & Applied Microbiology
Yi-Fan Yu, Jiashuo Yang, Fengguang Zhao, Ying Lin, Shuangyan Han
Summary: This study analyzed the integrity of the dissimilation pathway in Pichia pastoris using transcriptomics and metabolomics for the first time. Blocking the dissimilation pathway significantly decreased the level of oxidative phosphorylation and weakened the methanol assimilation pathway, resulting in insufficient energy supply and carbon fixation, leading to inefficient biomass accumulation and genetic replication. Additionally, the transcriptional upregulation of proteasomes and autophagy may be a stress response to resolve formaldehyde-induced DNA-protein crosslinking.
Article
Biochemistry & Molecular Biology
Kejie Li, Zhanqi Dong, Feifan Dong, Zhigang Hu, Liang Huang, Jie Wang, Peng Chen, Cheng Lu, Minhui Pan
Summary: Apoptosis is crucial for the normal growth, development, and immunity defense of living organisms. Viral infection triggers apoptosis in infected cells, but viruses have developed countermeasures to inhibit apoptosis in host cells. The study focuses on investigating the mechanism of how IAP2 induces apoptosis as well as the potential for creating resistant material through the knockout of BmNPV iap2.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Heikki V. Sarin, Eija Pirinen, Kirsi H. Pietilainen, Ville Isola, Keijo Hakkinen, Markus Perola, Juha J. Hulmi
Summary: This study on 42 healthy athletic females revealed that prolonged energy deficit can lead to metabolic adaptations, including suppression of mitochondrial and ribosome biogenesis gene expression. These findings suggest that adaptation to energy deficit in humans is broader than previously believed.
Article
Biochemistry & Molecular Biology
Yongjian Luo, Yixin Zhang, Jingyu Le, Qing Li, Jiaolin Mou, Shiming Deng, Jitao Li, Ru Wang, Zhijun Deng, Jun Liu
Summary: Metasequoia glyptostroboides, a critically endangered and protected species in China, is at risk of extinction due to seed aging. This study analyzed the antioxidant system and transcriptome of M. glyptostroboides seeds during artificial accelerated aging and revealed the physiological and molecular mechanisms. The results showed a significant decline in germination percentage and changes in antioxidant levels during the aging process. Differential gene expression analysis identified key pathways and hub genes involved in seed aging. These findings contribute to a better understanding of seed aging regulation and provide potential target genes for breeding endangered gymnosperms.
Article
Environmental Sciences
Yuying Ren, Yue Su, Wanwan Wang, Fanchi Li, Haina Sun, Bing Li
Summary: This study investigated the sublethal effects of ?-Cyhalothrin on silkworm larvae and found that it inhibited growth, increased mortality and abnormal pupa rates. Transcriptome sequencing revealed that energy and nutrient metabolisms were negatively affected, including the inhibition of the oxidative phosphorylation pathway and ATP synthesis.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Cell Biology
Peter Z. Schall, Keith E. Latham
Summary: Oogenesis is a complex process resulting in the production of a truly remarkable cell – the oocyte. Different species exhibit considerable divergence in oocyte transcriptome handling during maturation, but correct downregulation of mRNAs related to oxidative phosphorylation and mitochondrial function is a major shared feature of mammalian oocyte maturation.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Cell Biology
Meghan L. Ruebel, Filippo Zambelli, Peter Z. Schall, Montserrat Barragan, Catherine A. VandeVoort, Rita Vassena, Keith E. Latham
Summary: Research identified shared transcriptome abnormalities in rhesus monkey and human failed-to-mature (FTM) oocytes, including effects on mitochondrial function, oxidative phosphorylation (OxPhos), lipid metabolism, and other cellular functions. These results suggest fundamental aspects of cellular function are abnormal in FTM oocytes and raise concerns about using them for assisted reproduction technology (ART).
PHYSIOLOGICAL GENOMICS
(2021)
Article
Agriculture, Dairy & Animal Science
Y. Xue, Y. Yin, E. B. Trabi, F. Xie, L. Lin, S. Mao
Summary: The study found that both high-grain non-pelleted (HG) and high-grain pelleted (HP) diets significantly altered the transcriptome profiles of rumen epithelium in sheep. These diets inhibited protein synthesis and energy conversion in the ruminal epithelium. Compared to HG diet, HP diet showed lower growth benefits, induced more severe rumen acidosis, and more significantly inhibited energy production and protein synthesis.
Article
Engineering, Environmental
Qing Wang, Xin Wang, Daqi Zhang, Wensheng Fang, Yuan Li, Aocheng Cao, Qiuxia Wang, Dongdong Yan
Summary: The prevention and control of root-knot nematode disease is a global challenge. Fumigant dimethyl disulfide (DMDS) has excellent biological activity against nematodes, but it shows variations in contact and fumigation toxicity. This study investigated the specific regulatory mechanisms of DMDS on nematodes, revealing its effects on ultrastructure, physiological indicators, biochemical indicators, and vital signaling pathways.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Microbiology
Quanzhen Lv, Lan Yan, Jinxin Wang, Jia Feng, Lu Gao, Lijuan Qiu, Wen Chao, Yu-Lin Qin, Yuanying Jiang
Summary: The development of new antifungal drugs is crucial for addressing antifungal resistance and expanding the variety of clinical antifungal drugs. In this study, a new lead compound, xy12, was synthesized by modifying the pyrylium salt SM21, demonstrating effective antifungal activity against Candida species. The antifungal mechanism of xy12 was found to be the inhibition of mitochondrial complex I in Candida albicans.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Ludan Hou, Kexing Yan, Shuai Dong, Lifeng Guo, Jingyu Liu, Shurong Wang, Mingchang Chang, Junlong Meng
Summary: In this study, 614 DEGs specifically regulated by H2O2 were identified. GO-enrichment analysis showed that these DEGs were significantly enriched in multiple pathways related to ATP synthesis. Further KEGG analysis revealed that 23 DEGs were involved in cap color formation through the oxidative phosphorylation pathway. The results suggest that H2O2 may upregulate the expression of complex-encoding genes in the respiratory chain and promote ATP synthesis, thereby affecting the formation of cap color.
Article
Chemistry, Multidisciplinary
Jiayan Chen, Liping Lu, Chunlong Zhang, Xiaoming Zhu, Shulin Zhuang
Summary: In this study, it was found that black phosphorus nanomaterials have adverse effects on vasculature, with BPNSs being more likely to cause endothelial dysfunction and platelet adhesion. The impact on eNOS also differs between BPQDs and BPNSs. Although there was no significant structural damage in the mouse aortas, both BPQDs and BPNSs triggered aberrations in the aortic transcriptome, involving vasoconstriction abnormalities, metabolic disturbances, and immune perturbations.
Article
Endocrinology & Metabolism
Daniel C. Bittel, Adam J. Bittel, Arun S. Varadhachary, Terri Pietka, David R. Sinacore
Summary: Two-thirds of individuals with type 2 diabetes will develop chronic kidney disease, leading to early frailty, mobility deficits, and increased mortality risk. The mechanisms linking renal decline to mobility deficits are crucial for identifying high-risk individuals and formulating effective treatment strategies. Skeletal muscle energetics and mitochondrial function play a key role in impairing physical function in the progression of T2DM-induced CKD.
Article
Biodiversity Conservation
Sharon Renan, Edith Speyer, Tamar Ben-Nun, Alon Ziv, Gili Greenbaum, Alan R. Templeton, Shirli Bar-David, Amos Bouskila
BIOLOGICAL CONSERVATION
(2018)
Review
Behavioral Sciences
Oded Berger-Tal, Alison L. Greggor, Biljana Macura, Carrie Ann Adams, Arden Blumenthal, Amos Bouskila, Ulrika Candolin, Carolina Doran, Esteban Fernandez-Juricic, Kiyoko M. Gotanda, Catherine Price, Breanna J. Putman, Michal Segoli, Lysanne Snijders, Bob B. M. Wong, Daniel T. Blumstein
BEHAVIORAL ECOLOGY
(2019)
Editorial Material
Behavioral Sciences
Oded Berger-Tal, Alison L. Greggor, Biljana Macura, Carrie Ann Adams, Arden Blumenthal, Amos Bouskila, Ulrika Candolin, Carolina Doran, Esteban Fernandez-Juricic, Kiyoko M. Gotanda, Catherine Price, Breanna J. Putman, Michal Segoli, Lysanne Snijders, Bob B. M. Wong, Daniel T. Blumstein
BEHAVIORAL ECOLOGY
(2019)
Correction
Biochemistry & Molecular Biology
Amit Blumberg, Edward J. Rice, Anshul Kundaje, Charles G. Danko, Dan Mishmar
Article
Multidisciplinary Sciences
Gilad Barshad, Nicol Zlotnikov-Poznianski, Lihi Gal, Maya Schuldiner, Dan Mishmar
SCIENTIFIC REPORTS
(2019)
Review
Ecology
Noam Shtolz, Dan Mishmar
FRONTIERS IN ECOLOGY AND EVOLUTION
(2019)
Article
Zoology
Guy Sion, Maggie J. Watson, Amos Bouskila
Summary: Condition indices (CIs) are used in ecological studies to measure the health and fitness of individual animals, often validated invasively by fat extraction. Dual-energy X-ray absorptiometry (DXA) allows for non-destructive analysis of body composition and multiple measurements. This study calibrated DXA against a chemical extraction of fat and found that the ratio of body mass to SVL is the most accurate body condition estimator for this species.
FRONTIERS IN ZOOLOGY
(2021)
Article
Ecology
Adi Domer, Ehud Vinepinsky, Amos Bouskila, Eyal Shochat, Ofer Ovadia
Summary: During migration, birds stop at different quality stopover sites and their decision to stay or move depends on their body condition to maximize fuel load for successfully crossing ecological barriers.
JOURNAL OF ANIMAL ECOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Tania L. F. Bird, Pua Bar (Kutiel), Elli Groner, Amos Bouskila
Summary: The positive relationship between diversity and stability is well-supported, with asynchrony playing a key role in driving community stability for both flora and fauna. Richness and environmental conditions have varying effects on stability across different taxonomic groups, highlighting the importance of species interactions and responses to the environment in determining stability.
APPLIED SCIENCES-BASEL
(2021)
Article
Ecology
Shimon Bershtein, Daniel Kleiner, Dan Mishmar
Summary: Recent advances in AI-based 3D protein structure prediction have the potential to address health-related questions and impact evolution. Integration of structural predictions with functional genomic studies is emphasized for a comprehensive understanding of protein properties and their effects on fitness.
NATURE ECOLOGY & EVOLUTION
(2021)
Article
Microbiology
Dan T. Bar Yaacov
Summary: The involvement of ADARs in regulating anti-viral responses is conserved in planarians, and it suggests that ADARs' role in suppressing abnormal anti-viral responses is an ancient evolutionary invention shared by many organisms.
Article
Multidisciplinary Sciences
Hadar Medini, Amit Zirman, Dan Mishmar
Summary: Mitochondrial dysfunction has been found to play a central role in COVID-19, with significantly reduced mtDNA gene expression in patients observed, suggesting a potential link to the disease. Additionally, elevated expression of ROS-response genes and glycolysis enzymes in patients may indicate a shift towards glycolysis, creating conditions beneficial for SARS-CoV-2 replication.
Article
Biochemistry & Molecular Biology
Tal Cohen, Hadar Medini, Chen Mordechai, Alal Eran, Dan Mishmar
Summary: By analyzing a large number of RNA-seq samples, we found that modifications in mt-RNA are negatively correlated with mtDNA gene expression levels, and this correlation varies across different tissues. Additionally, we observed that environmental factors can affect the impact of these modifications on gene expression.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
Article
Biology
Noam Shtolz, Dan Mishmar
Summary: By analyzing mtDNAs from about 8000 metazoans, it was found that most metazoans have highly conserved gene content and codon preferences in their mtDNA. However, gene order (MGO) is selectively constrained within but not between phyla, and certain gene stretches are highly conserved across metazoans. The analysis of RNA-seq data from 53 metazoans suggests that mtDNA rearrangements impact mtDNA regulation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Shani Marom, Amit Blumberg, Anshul Kundaje, Dan Mishmar