期刊
JOURNAL OF VIROLOGICAL METHODS
卷 211, 期 -, 页码 55-62出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2014.10.010
关键词
Dengue virus; Viral reporter; Protease; Fluorescence microscopy
资金
- NIH [P01 AI034533, P20 GM103430-12, P20 COBRE GM 104317 U01 AI070484]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [P01AI034533, U01AI070484] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P20GM104317, P20GM103430] Funding Source: NIH RePORTER
Cell culture models are used widely to study the effects of dengue virus (DENV) on host cell function. Current methods of identification of cells infected with an unmodified DENV requires fixation and permeablization of cells to allow DEN V-specific antibody staining. This method does not permit imaging of viable cells over time. In this report, a plasmid-based reporter was developed to allow non-destructive identification of DENV-infected cells. The plasmid-based reporter was demonstrated to be broadly applicable to the four DENV serotypes, including low-passaged strains, and was specifically cleaved by the viral protease with minimal interference on viral production. This study reveals the potential for this novel reporter system to advance the studies of virus-host interactions during DENV infection. (C) 2014 Elsevier B.V. All rights reserved.
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