4.1 Article

Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers

期刊

GENETICS AND MOLECULAR RESEARCH
卷 10, 期 4, 页码 2366-2381

出版社

FUNPEC-EDITORA
DOI: 10.4238/2011.October.6.2

关键词

Microsatellite marker; Coffea; Discriminant analysis; Genetic diversity

资金

  1. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
  2. Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)
  3. Consorcio Brasileiro de Pesquisa e Desenvolvimento do Cafe (CBP D)
  4. Financiadora de Estudos e Projetos (FINEP)

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Coffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian agricultural production, generating a revenue of US$6 billion. Despite the importance of coffee production in Brazil, it is supported by a narrow genetic base, with few accessions. Molecular differentiation and diversity of a coffee breeding program were assessed with gSSR and EST-SSR markers. The study comprised 24 coffee accessions according to their genetic origin: arabica accessions (six traditional genotypes of C. arabica), resistant arabica ( six leaf rust-resistant C. arabica genotypes with introgression of Hibrido de Timor), robusta (five C. canephora genotypes), Hibrido de Timor (three C. arabica x C. canephora), triploids (three C. arabica x C. racemosa), and racemosa (one C. racemosa). Allele and polymorphism analysis, AMOVA, the Student t-test, Jaccard's dissimilarity coefficient, cluster analysis, correlation of genetic distances, and discriminant analysis, were performed. EST-SSR markers gave 25 exclusive alleles per genetic group, while gSSR showed 47, which will be useful for differentiating accessions and for fingerprinting varieties. The gSSR markers detected a higher percentage of polymorphism among (35% higher on average) and within (42.9% higher on average) the genetic groups, compared to EST-SSR markers. The highest percentage of polymorphism within the genetic groups was found with gSSR markers for robusta (89.2%) and for resistant arabica (39.5%). It was possible to differentiate all genotypes including the arabica-related accessions. Nevertheless, combined use of gSSR and EST-SSR markers is recommended for coffee molecular characterization, because EST-SSRs can provide complementary information.

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