Article
Plant Sciences
Chakravarthi Mohan, Priscila Yumi Tanaka Shibao, Fernando Fonseca Pereira de Paula, Danyelle Toyama, Marcos Antonio Sanches Vieira, Antonio Figueira, Danielle Scotton, Andrea Soares-Costa, Flavio Henrique-Silva
Summary: The study demonstrates that down-regulation of the key gene V-ATPase E in sugarcane weevil using RNA interference can effectively inhibit its growth and survival, providing a promising approach for controlling sugarcane weevil infestations.
PLANT CELL REPORTS
(2021)
Article
Agronomy
Mateus Salviano Oliveira Silva, Jorge Franco Maringoli Cardoso, Maria Elizia Pacheco Ferreira, Fernando Berton Baldo, Raphael Satochi Abe Silva, Julie Giovanna Chacon-Orozco, David I. Shapiro-Ilan, Selcuk Hazir, Cesar Junior Bueno, Luis Garrigos Leite
Summary: Several nematodes were compared for their ability to search for insect larvae in sugarcane rhizomes, with native Steinernema rarum showing superior virulence against pests like the sugarcane billbug. Field trials showed that S. rarum effectively controlled pests like S. levis, L. alvarengai, and H. taltula, indicating its potential as a biocontrol agent in sugarcane fields.
Article
Entomology
Pedro Henrique Urach Ferreira, Marcelo da Costa Ferreira
Summary: This study evaluated the behavior of the sugarcane weevil, Sphenophorus levis, and found that it is not attracted or repelled by the recommended insecticide dose. The weevils are primarily nocturnal, with increased activity and exposure at night. Therefore, nocturnal insecticide applications may be more effective for controlling S. levis.
Article
Biochemistry & Molecular Biology
Priscila Yumi Tanaka Shibao, Milene Ferro, Fernando Fonseca Pereira de Paula, Bruno Salata Lima, Flavio Henrique-Silva
Summary: The main digestive protease Sl-CathL-CS of Sphenophorus levis was identified as a pseudo-cysteine protease that assists in protein digestion by interacting with sugarcane cystatin inhibitors. The study revealed that the mutant Sl-CathL-mutSC, with a reverted serine residue to cysteine, showed protease activity and was inhibited by a cysteine protease inhibitor. Protein ligand docking further highlighted the differences in catalytic sites between native and mutant proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Jia Zhang, Zhijun Luo, Nan Li, Yongqiang Yu, Minmin Cai, Longyu Zheng, Fengling Zhu, Feng Huang, Jeffery K.Tomberlin, Kashif ur Rehman, Ziniu Yu, Jibin Zhang
Summary: The study reveals the importance of mixing chicken manure into dairy manure for improving the efficiency of black soldier fly larvae in converting manure. It also highlights the role of intestinal microbiota in cellulose degradation and biomass production. This research provides insights into the microecological mechanisms underlying the efficient conversion of cellulose-rich manure by black soldier fly larvae.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Materials Science, Paper & Wood
Ming Yang, Jingjing Zhao, Yue Yuan, Xiaoyi Chen, Fan Yang, Xianzhen Li
Summary: In this study, a microbiota (FPDM) with cellulose-degrading ability was cultivated to investigate the dynamic process of cellulose biodegradation and explore potential cellulases. The results showed that Sporocytophaga and Cohnella dynamically dominated the synergistic degradation of cellulose in different periods, while unannotated genes were speculated to participate in cellulose catabolism. The dynamic cellulose-degrading pathway of FPDM was predicted based on changes in hydrolysates, community structure, and gene abundance, providing a new perspective for identifying key cellulolytic strains and enzymes.
Article
Biotechnology & Applied Microbiology
Luis G. Leite, Julie G. Chacon-Orozco, David I. Shapiro-Ilan, Fernando B. Baldo, Jorge F. M. Cardoso
Summary: This study tested and optimized a biphasic process for producing Steinernema rarum nematodes and demonstrated that the phenolic sponge biphasic system is a highly efficient approach for production of entomopathogenic nematodes. The produced nematodes showed significant control efficacy against the sugarcane billbug.
BIOLOGICAL CONTROL
(2023)
Article
Multidisciplinary Sciences
Su Sun, Fei Li, Muzi Li, Wenqian Zhang, Zhenxiong Jiang, Honglu Zhao, Yunqiao Pu, Arthur J. Ragauskas, Susie Y. Dai, Xiaoyu Zhang, Hongbo Yu, Joshua S. Yuan, Shangxian Xie
Summary: LPMOs can synergize with lignin-degrading enzymes for efficient lignin degradation in white-rot fungi. This study reveals the versatile roles of LPMOs in the redox network and their ability to dynamically and efficiently degrade different cell wall components.
Article
Microbiology
Andrea Pacetti, Samuele Moretti, Celia Perrin, Eric Gelhaye, Evi Bieler, Hanns-Heinz Kassemeyer, Laura Mugnai, Sibylle Farine, Christophe Bertsch
Summary: Fomitiporia mediterranea is a fungus associated with decay in grapevine wood, and its degradation pathways and enzyme activity show significant intraspecific variability. The study suggests that its role in foliar symptoms may have been underestimated, and fungal strain and vine variety are important factors in wood degradation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Polymer Science
Andres Felipe Ochica Larrota, Ricardo Vera-Graziano, Alex Lopez-Cordoba, Edwin Yesid Gomez-Pachon
Summary: This study extracted cellulose from sugarcane bagasse and fabricated acetate cellulose fibers through electrospinning, followed by functionalization and coloring with anionic dye. The results showed that cationic cellulose fibers dyed without added salt exhibited better color fixation and morphology preservation, indicating potential for textile applications.
Article
Chemistry, Applied
Lu Yao, Songnan Hu, Xijun Wang, Minsheng Lin, Cunzhi Zhang, Yian Chen, Fengxia Yue, Haisong Qi
Summary: Lignin-containing cellulose nanofibrils (LCNFs) with tunable lignin contents were directly prepared from sugarcane bagasse. LCNFs showed a more uniform size and narrow diameter distribution compared to cellulose nanofibrils (CNFs). CNPs made from LCNFs exhibited outstanding mechanical and optical properties, and LCNFs had higher water contact angles than CNFs.
CARBOHYDRATE POLYMERS
(2022)
Article
Environmental Sciences
Hao Li, Xueming Xu, Minqi Zhang, Yuanhao Zhang, Ying Zhao, Xueping Jiang, Xiangdong Xin, Zhendong Zhang, Ran Zhang, Zhongzheng Gui
Summary: Housefly larvae vermicomposting can significantly accelerate the degradation of cellulose in silkworm excrement by increasing the abundance of cellulose-degrading bacterial genera and cellulase.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Review
Energy & Fuels
Paul Wolski, Brian W. Blankenship, Athiyya Umar, Mica Cabrera, Blake A. Simmons, Kenneth L. Sale, Ezinne C. Achinivu
Summary: Ionic liquids are considered a more sustainable alternative to volatile organic solvents and are driving innovations in industries such as energy storage, separations, and bioprocessing. However, their limited biocompatibility and negative impact on enzyme activity and stability pose challenges in the conversion of lignocellulosic biomass to biofuels and bioproducts. Efforts have been made to discover compatible enzyme-IL systems and improve understanding of the effects of ILs on these systems. This review examines the impact of ionic liquids on enzymes in lignocellulosic biomass deconstruction, with a focus on their relevance in pretreatment.
FRONTIERS IN ENERGY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Neal N. Hengge, Sam J. B. Mallinson, Patthra Pason, Vladimir V. Lunin, Markus Alahuhta, Daehwan Chung, Michael E. Himmel, Janet Westpheling, Yannick J. Bomble
Summary: Microbial conversion of biomass depends on complex enzyme systems. Some thermophilic bacteria have high cellulolytic activity. GuxA, a multi-modular and multi-functional enzyme from Acidothermus cellulolyticus, exhibits synergism with other enzymes and has potential for biomass conversion. The study of GuxA provides insights into its activity mechanism and substrate specificity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Cibele Carneiro Pessan, Juliana Silva Bernardes, Silvia H. P. Bettini, Edson R. Leite
Summary: Understanding the relationship between cellulose fiber morphology and the rheological behavior of its dispersions is important for optimizing their applications. Sugarcane bagasse-sourced cellulose dispersions with different numbers of high-pressure homogenization cycles were produced. Microfluidization produced cellulose nanofibers with similar surface charge densities and crystallinities. The rheological properties of the dispersions could be tuned by adjusting the length of the nanofibers, allowing for targeted applications.
CARBOHYDRATE POLYMERS
(2023)
Article
Entomology
Fernanda Canassa, Fernanda C. N. Esteca, Rafael A. Moral, Nicolai Meyling, Ingeborg Klingen, Italo Delalibera
JOURNAL OF PEST SCIENCE
(2020)
Article
Biodiversity Conservation
Fernanda de Cassia Neves Esteca, Nina Trandem, Ingeborg Klingen, Jandir Cruz Santos, Italo Delalibera Junior, Gilberto Jose de Moraes
Review
Entomology
Ann E. Hajek, Sana Gardescu, Italo Delalibera
Summary: The 2016 catalogue of classical biological control introductions of entomopathogens and nematodes targeting arthropod pests in 55 countries revealed that some programs using pathogens highly specific to pest species have been highly successful and should be further explored.
Article
Entomology
M. R. Abreu, I. Delalibera, N. R. C. Pereira, M. I. Camargo-Mathias
Summary: The study demonstrated that exposure to ozonated water at different concentrations can lead to severe alterations in the salivary glands of Rhipicephalus sanguineus sensu lato, offering a new ecologically friendly control method that poses no harm to non-target organisms and the environment.
MEDICAL AND VETERINARY ENTOMOLOGY
(2021)
Article
Immunology
Gabriel Moura Mascarin, Natasha Sant'Anna Iwanicki, Jose Luis Ramirez, Italo Delalibera, Christopher A. Dunlap
Summary: Culturing Beauveria bassiana under high glucose concentrations and high aeration leads to a shift in fungal development towards blastospore production, with associated differential gene expression related to antioxidant, calcium transport, conidiation, and osmosensor-related genes. The highly expressed Hyd1 gene suggests involvement in spore coat assembly, and upregulation of HOG signaling pathway genes points to potential mechanisms behind blastospore development for industrial pest control applications.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Natasha Sant'Anna Iwanicki, Gabriel Moura Mascarin, Sara Giro Moreno, Jorgen Eilenberg, Italo Delalibera
Summary: The research investigated the spray-drying and air-drying techniques applied to Metarhizium robertsii blastospores to develop wettable powder formulations. Various co-formulants were tested for their effect on viability and wettability of the blastospores. Oxygen-moisture absorbers were found to significantly increase the shelf life of the formulations at different storage temperatures.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Genetics & Heredity
Natasha Sant'Anna Iwanicki, Ana Beatriz Riguetti Zanardo Botelho, Ingeborg Klingen, Italo Delalibera Junior, Simeon Rossmann, Erik Lysoe
Summary: The genus Metarhizium consists of species used in agricultural pest control programs and has both insect pathogens and plant growth-promoting endophytes. This study sequenced the genome of a newly described species, M. humberi, and compared it to other Metarhizium species. The genome analysis revealed unique traits and provided insights into the broad host range and potential biological functions of M. humberi.
G3-GENES GENOMES GENETICS
(2022)
Article
Microbiology
Daniela Milanez Silva, Victor Hugo Moura de Souza, Rafael de Andrade Moral, Italo Delalibera Junior, Gabriel Moura Mascarin
Summary: In this study, the effect of different liquid media on the growth of P. lilacinum and P. chlamydosporia was assessed. The most promising isolates were found to promote plant growth and control pests. The results demonstrated the possibility of producing high concentrations of spores through liquid culture and the effectiveness of seed treatment in reducing pest populations.
Article
Microbiology
Mariana O. G. Leite, Denise A. Alves, Antoine Lecocq, Jose Bruno Malaquias, Italo Delalibera, Annette B. Jensen
Summary: The use of fungal-based biopesticides is considered a low-risk control strategy to reduce pest damage and protect crop quality. However, a risk assessment is still needed for mycopesticides as pests and beneficial insects coexist in the same agroecosystem where this strategy is used.
Article
Ecology
Joel da Cruz Couceiro, Henrik H. De Fine Licht, Italo Delalibera Jr., Nicolai V. Meyling
Summary: This study examines the genetic variability and adaptation of different species within the fungal genus Metarhizium to different habitats. The results show that the variability is associated with the geographic origin of the isolates and their ability to infect insects and colonize plant roots.
Article
Genetics & Heredity
Natasha SantAnna Iwanicki, Italo Delalibera Junior, Lana L. B. de Carvalho, Jorgen Eilenberg, Henrik H. De Fine Licht
Summary: In this study, it was found that M. rileyi yeast-like cells produced on solid medium showed comparable efficiency in infecting and killing Spodoptera frugiperda, an important insect pest, as yeast-like cells grown in liquid medium. Transcriptomic analysis revealed upregulated genes related to signal transduction and membrane transporters in yeast-like cells grown in liquid medium. Comparison of transcriptomic profiles between M. rileyi and M. anisopliae yeast-like cells showed more differences than similarities.
FUNGAL GENETICS AND BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Sabrina Holz, Celeste Paola D'Alessandro, Heros Jose Maximo, Paulo Henrique Nascimento de Souza, Yenjit Raruang, Clarice Garcia Borges Demetrio, Italo Delalibera Junior, Zhi-Yuan Chen, Sergio Florentino Pascholati
Summary: The biocontrol efficacy of two strains of Metarhizium (MABR-01 and MHBR-03) against Asian soybean rust was evaluated in this study. The culture filtrates of M. anisopliae and M. humberi at a 50% concentration inhibited the germination of P. pachyrhizi spores by 85% and 96%, respectively, while the conidial suspensions had no inhibitory effect. However, the conidial suspension of M. anisopliae showed a 51% efficiency in controlling rust disease in plants. Spraying plants with culture filtrates of M. humberi and M. anisopliae significantly reduced rust disease by an average of 86%.
BIOCONTROL SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Isabella Alice Gotti, Camila Costa Moreira, Italo Delalibera, Henrik H. De Fine Licht
Summary: Infective conidia are widely used for biological control of insects, but the yeast-like cells called blastospores also have potential. This study compared blastospores and conidia from two different Metarhizium fungi and found that M. rileyi blastospores had higher virulence towards S. frugiperda than M. anisopliae blastospores and conidia. Comparative transcriptomics also showed that M. rileyi blastospores expressed more virulence-related genes.
Article
Food Science & Technology
Ana Carolina Oliveira Siqueira, Gabriel Moura Mascarin, Cassiara R. N. C. B. Goncalves, Joelma Marcon, Maria Carolina Quecine, Antonio Figueira, Italo Delalibera
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2020)
Article
Agriculture, Multidisciplinary
Anja Amtoft Wynns, Annette Bruun Jensen, Jorgen Eilenberg, Italo Delalibera Junior