期刊
GENETICS AND MOLECULAR BIOLOGY
卷 35, 期 1, 页码 18-26出版社
SOC BRASIL GENETICA
DOI: 10.1590/S1415-47572012005000009
关键词
CNE2 cell line; nasopharyngeal carcinoma; nude mouse; STGC3; Tet-on; two-dimensional electrophoresis
资金
- National Natural Science Foundation of China [81172575]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20104324110002]
- Hunan Province Natural Sciences Foundation of China [09JJ3071]
- Hunan Province Education Department Foundation of China [08A060]
STGC3 is a potential tumor suppressor that inhibits the growth of the nasopharyngeal carcinoma cell line CNE2; the expression of this protein is reduced in nasopharyngeal carcinoma compared with normal nasopharyngeal tissue. In this study, we investigated the tumor-suppressing activity of STGC3 in nude mice injected subcutaneously with Tet/pTRE-STGC3/CNE2 cells. STGC3 expression was induced by the intraperitoneal injection of doxycycline (Dox). The volume mean of Tet/pTRE-STGC3/CNE2+Dox xenografts was smaller than that of Tet/pTRE/CNE2+Dox xenografts. In addition, Tet/pTRE-STGC3/CNE2+Dox xenografts showed an increase in the percentage of apoptotic cells, a decrease in Bcl-2 protein expression and an increase in Bax protein expression. A proteomic approach was used to assess the protein expression profile associated with STGC3-mediated apoptosis. Western blotting confirmed the differential up-regulation of prohibitin seen in proteomic analysis. These results indicate that overexpression of STGC3 inhibits xenograft growth in nude mice by enhancing apoptotic cell death through altered expression of apoptosis-related proteins such as Bcl-2, Bax and prohibitin. These data contribute to our understanding of the function of STGC3 in human nasopharyngeal carcinoma and provide new clues for investigating other STGC3-associated tumors.
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