期刊
GENETICS AND MOLECULAR BIOLOGY
卷 32, 期 1, 页码 58-68出版社
SOC BRASIL GENETICA
DOI: 10.1590/S1415-47572009000100009
关键词
constitutive heterochromatin; in situ restriction endonuclease digestion; Rodentia
资金
- Science and Technology Foundation (FCT), Portugal [POCI/BIABCM/58541/2004, SFRH/BD/41574/2007]
The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin revealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the karyotype of this species is the more derived when compared with the other two genomes analyzed, probably originated by a great number of complex chromosomal rear-rangements. The high level of sequence heterogeneity identified in the CH of the three genomes suggests the coexistence of different satellite DNA families, or variants of these families in these genomes.
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