期刊
GENETICS
卷 195, 期 3, 页码 635-642出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.113.156521
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资金
- Howard Hughes Medical Institute Funding Source: Medline
- NIGMS NIH HHS [5R01GM095817-03, R01 GM095817] Funding Source: Medline
With remarkable speed, the CRISPR-Cas9 nuclease has become the genome-editing tool of choice for essentially all genetically tractable organisms. Targeting specific DNA sequences is conceptually simple because the Cas9 nuclease can be guided by a single, short RNA (sgRNA) to introduce double-strand DNA breaks (DSBs) at precise locations. Here I contrast and highlight protocols recently developed by eight different research groups, six of which are published in GENETICS, to modify the Caenorhabditis elegans genome using CRISPR/Cas9. This reverse engineering tool levels the playing field for experimental geneticists.
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