期刊
GENETICS
卷 188, 期 1, 页码 229-U354出版社
GENETICS SOC AM
DOI: 10.1534/genetics.110.126193
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资金
- Eunice Kennedy Shriver National Institute of Child Health and Human Developmen [HD008748]
- National Institute of Mental Health [MH-002800-07]
eHere we report the development of a ternary version of the LexA::VP16/LexAop system in which the DNA-binding and trans-activating moieties are independently targeted using distinct promoters to achieve highly restricted, intersectional expression patterns. This Split LexA system can be concatenated with the Gal4/upstream activating sequence system to refine the expression patterns of existing Gal4 lines with minimal genetic manipulations.
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