期刊
GENETICS
卷 186, 期 4, 页码 1175-1185出版社
OXFORD UNIV PRESS INC
DOI: 10.1534/genetics.110.122234
关键词
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资金
- National Natural Science Foundation of China
- National Key Basic Research project [2010CB126306]
- Program of Wuhan Subject Chief Scientist
- 111 project [B06018]
Piwi proteins and their partner small RNAs play an essential role in fertility, germ-line stem cell development, and the basic control and evolution of animal genomes. However, little knowledge exists regarding piRNA biogenesis. Utilizing microfluidic chip analysis, we present a quantitative profile of zebrafish piRNAs expressed differentially between testis and ovary. The sex-specific piRNAs are derived from separate loci of repeat elements in the genome. Ovarian piRNAs can be categorized into groups that reach up to 92 members, indicating a sex-specific arrangement of piRNA genes in the genome. Furthermore, precursor piRNAs preferentially form a hairpin structure at the 3' end, which seem to favor the generation of mature sex-specific piRNAs. In addition, the mature piRNAs from both the testis and the ovary are 2'-O-methylated at their 3' ends.
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