期刊
GENETICS
卷 180, 期 3, 页码 1763-1766出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.108.094508
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资金
- NIGMS NIH HHS [R01 GM061936, F32GM067460, R01GM61936, F32 GM067460] Funding Source: Medline
Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the Phi C31 integrase. This PCR-based strategy employs small attB tails on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.
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