Article
Biotechnology & Applied Microbiology
Qiutao Ding, Runsheng Li, Xiaoliang Ren, Lu-Yan Chan, Vincy W. S. Ho, Dongying Xie, Pohao Ye, Zhongying Zhao
Summary: This study uses nanopore DNA sequencing and short reads to investigate the architecture and variation of the 5S rDNA cluster in nematodes. The results show that rDNA units are highly dynamic in terms of sequence and copy number and that the widely used N2 strain is defective in maintaining the stability of the 5S rDNA cluster compared to other wild isolates.
Article
Plant Sciences
Anton Stepanenko, Guimin Chen, Phuong T. N. Hoang, Joerg Fuchs, Ingo Schubert, Nikolai Borisjuk
Summary: This study comprehensively characterized the chromosomal locations and molecular organization of 35S and 5S rDNA genes in water lettuce using karyological and molecular methods. Single locations for the 35S and 5S rDNA loci were identified using fluorescence in situ hybridization. The molecular cloning and nucleotide sequencing of 35S rDNA revealed typical structural characteristics and allowed for phylogenetic analysis. The 5S rDNA locus showed variation in nucleotide composition and subrepeat number, confirming the mosaic composition of the gene locus.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Horticulture
Guangxin Liu, Yue Lan, Lianwei Qu, Yilian Zhao, Haoyang Xin, Mengli Xi
Summary: Through our research on wild species and cultivars, we have clarified the genetic relationships between different sections of tulips, as well as the differences and variations in the 5S rDNA sequences. Our findings provide clearer and more stable evidence for the classification of tulips.
SCIENTIA HORTICULTURAE
(2022)
Article
Parasitology
Tomas Scholz, Andrea Waeschenbach, Mikulas Oros, Jan Brabec, D. Timothy J. Littlewood
Summary: This study presents the most comprehensive multi-gene molecular phylogeny of tapeworms of the order Caryophyllidea, revealing three well-supported lineages and highlighting the importance of host association and biogeographical distribution in their classification.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2021)
Article
Limnology
LIU Yu, LIU Pengfei, BI Yanhui, ZHOU Zhigang
Summary: The linkage of 5S rRNA gene to 18S-5.8S-25S rRNA gene was reported in some species in Ochrophyta. Dual-color fluorescence in situ hybridization technique revealed the localization and physical unlinkage of rRNA genes in Saccharina japonica, providing insights into the cytogenetics of this kelp species.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2021)
Article
Genetics & Heredity
Jun He, Yong Zhao, Shuangshuang Zhang, Yanze He, Jiafu Jiang, Sumei Chen, Weimin Fang, Zhiyong Guan, Yuan Liao, Zhenxing Wang, Fadi Chen, Haibin Wang
Summary: This study used oligonucleotide fluorescence in situ hybridization (Oligo-FISH) to draw ideograms of rDNA sites in Chrysanthemum accessions and found polymorphisms and co-localization of rDNA sites. There was also a correlation between the number of 5S rDNA sites and chromosome number, and the geographical distribution of different ploidy Chrysanthemum accessions may have promoted karyotype evolution. The study identified the formation mechanism of rDNA variations and provided a reliable method for examining rDNA variations in Chrysanthemum and related species, contributing to understanding the evolutionary and phylogenetic relationships of the Chrysanthemum genus.
Article
Veterinary Sciences
Mario Santoro, Marialetizia Palomba, Renato Aco Alburqueque, Simonetta Mattiucci
Summary: The Gymnorhynchidae family is a cestode family that includes three genera and six species. They are usually intestinal parasites of large pelagic sharks. These parasites have a wide distribution and pose a global sanitary concern as their larvae infect the edible flesh of commercially important fishes. In this study, larvae of Gymnorhynchidae were collected from Atlantic pomfret in the Mediterranean Sea and identified using various methods. The phylogenetic analysis revealed the relationship between different species and confirmed the co-infection of Molicola uncinatus and Gymnorhynchus gigas in all studied localities. The study also expanded the knowledge of intermediate hosts and geographical range of Molicola uncinatus. The results supported the suggestion that Gymnorhynchus gigas and Molicola species should be classified under the same genus. This study highlights the importance of integrative taxonomy in identifying trypanorhynch species and understanding the ecological and biological aspects of Gymnorhynchidae members.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jun He, Sisi Lin, Zhongyu Yu, Aiping Song, Zhiyong Guan, Weimin Fang, Sumei Chen, Fei Zhang, Jiafu Jiang, Fadi Chen, Haibin Wang
Summary: Using rDNA as a FISH probe, researchers found that 5S rDNA sites are mainly located in subterminal chromosome regions, while the number and intensity of 45S rDNA sites differ from previously studied Chrysanthemum resources. These findings offer insight into the chromosomal structural variations in Chrysanthemum and its related species.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Horticulture
Deen Mohammad Deepo, Md Mazharul Islam, Reshma Yesmin, Hye-Min Lee, Hong-Yul Kim, Chang-Kil Kim, Ki-Byung Lim
Summary: In this study, the nuclear DNA content, genome size, ploidy degree, and cytogenetic properties of two Korean native cultivars of Hibiscus 'Gangneung' and 'Baekryungdo' were determined. The flow cytometry results showed that the 2C-DNA contents of both cultivars were nearly identical. Fluorescence in situ hybridization (FISH) technique revealed the presence of 5S and 18S rDNA loci in both cultivars. This cytogenetic assessment of the cultivars will contribute to plant breeding within this genus.
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2023)
Article
Fisheries
Asit Kumar Bera, Nilemesh Das, Shreya Bhattacharya, Ramesh Chandra Malick, Himanshu Sekhar Swain, Hemanta Chowdhury, Archana Sinha, Sanjib Kumar Manna, Uttam Kumar Sarkar, Basanta Kumar Das
Summary: The study revealed that the one-stripe spiny eel encountered Clinostomum complanatum metacercariae infection during acclimatization, significantly impacting the survival rate of this fish species. The research not only identified the parasite type through light microscopy, but also molecularly confirmed the identification through PCR amplification and sequencing of the 18S RNA gene.
AQUACULTURE RESEARCH
(2021)
Article
Parasitology
Soo Lim Kim, Jun Ho Choi, Myung-hee Yi, Seogwon Lee, Myungjun Kim, Singeun Oh, In-Yong Lee, Bo-Young Jeon, Tai-Soon Yong, Ju Yeong Kim
Summary: This study used NGS-based metabarcoding analysis to examine the gut microbiome of the striped field mouse and found that microbial composition varied with season and parasitic infection status. The results highlight the advantages of NGS technology in monitoring zoonotic disease reservoirs.
PARASITES & VECTORS
(2022)
Article
Plant Sciences
Angeles Cuadrado, Marta Sixto, Rosa I. Figueroa, Isabel Bravo, Alfredo de Bustos
Summary: Through FISH analysis, we found differences in chromosomal organization and rDNA repeat unit numbers among different Gambierdiscus species. The location and organization of 5S and 45S rDNA in the nucleus also vary among species. These species can be distinguished by their species-specific FISH rDNA phenotypes.
EUROPEAN JOURNAL OF PHYCOLOGY
(2022)
Article
Horticulture
Woo-Young Cho, Deen Mohammad Deepo, Md Mazharul Islam, Si-Chun Nam, Hong-Yul Kim, Jeung-Sul Han, Chang-Kil Kim, Mi-Young Chung, Ki-Byung Lim
Summary: This study demonstrated that tetraploid 'Chammel' melons treated with oryzalin exhibited larger seeds and leaves, longer stomata with lower density, increased chromosome numbers, and doubled rDNA loci compared to diploids, suggesting the potential for generating new seedless oriental melon cultivars through polyploidization.
HORTICULTURAL SCIENCE & TECHNOLOGY
(2021)
Article
Entomology
Natalia Golub, Viktor B. Golub, Boris A. Anokhin, Valentina G. Kuznetsova
Summary: Tingidae, or lace bugs, is a family of herbivorous true bugs with approximately 2600 identified species. This study examined the karyotypes of 16 lace bug species and analyzed the information available on lace bug karyotypes. The study found that lace bugs have conservative karyotypes, different sex chromosome systems, and different patterns of 18S rDNA chromosomal localization. The search for chromosomal landmarks is important for further understanding lace bug cytogenetics.
Article
Zoology
Leandro Ranucci, Carlos A. Fernandes, Luciana A. Borin-Carvalho, Isabel C. Martins-Santos, Ana L. B. de Portela-Castro
Summary: This study presents the first cytogenetic data of two allopatric populations of Moenkhausia bonita and one of M. forestii, revealing differences in chromosome number, karyotype formula, and B chromosomes between the two species. These distinctions may contribute as a reference in taxonomic studies of this genus, providing insights into the evolutionary and cytotaxonomic aspects.
NEOTROPICAL ICHTHYOLOGY
(2021)
Article
Cell Biology
Naveen K. Somanna, Indra Mani, Satyabha Tripathi, Kailash N. Pandey
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2018)
Article
Cell Biology
Indra Mani, Kailash N. Pandey
CELLULAR SIGNALLING
(2019)
Review
Cell Biology
Sunil Kumar, Parth Sarthi, Indra Mani, Muhammad Umer Ashraf, Myeong-Ho Kang, Vishal Kumar, Yong-Soo Bae
Summary: Cellular immunotherapy has become a key player in cancer treatment, but some patients develop resistance to it, necessitating the search for more advanced treatments. Research suggests that targeting N(6)A modification mechanisms can enhance the efficacy of ICB therapy, by addressing issues related to high-dose antibody toxicity and drug resistance.
Review
Chemistry, Multidisciplinary
Gargi Bhattacharjee, Nisarg Gohil, Khushal Khambhati, Indra Mani, Rupesh Maurya, Janardhan Keshav Karapurkar, Jigresh Gohil, Dinh-Toi Chu, Hue Vu-Thi, Khalid J. Alzahrani, Pau-Loke Show, Rakesh M. Rawal, Suresh Ramakrishna, Vijai Singh
Summary: A single gene mutation can cause human diseases. The CRISPR-Cas9 system is a powerful tool for correcting these mutations and has been used in various diseases. Effective delivery of CRISPR components is crucial.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Biotechnology & Applied Microbiology
Khushal Khambhati, Gargi Bhattacharjee, Nisarg Gohil, Gurneet K. Dhanoa, Antonia P. Sagona, Indra Mani, Nhat Le Bui, Dinh-Toi Chu, Janardhan Keshav Karapurkar, Su Hwa Jang, Hee Yong Chung, Rupesh Maurya, Khalid J. Alzahrani, Suresh Ramakrishna, Vijai Singh
Summary: Antibiotic resistance is a major threat to humanity, and the use of bacteriophages as an alternative to antibiotics has gained interest. CRISPR-Cas9-assisted phage engineering provides a more specific targeting of bacterial pathogens. Techniques to enhance the fitness, specificity, and lytic ability of phages can be used to control infections.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Madan L. Khurana, Indra Mani, Prerna Kumar, Chandramohan Ramasamy, Kailash N. Pandey
Summary: This study investigated the ANP-dependent downregulation of NPRA in different cell types, revealing the underlying mechanisms and biochemical changes involved in the process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Meeting Abstract
Physiology
Chandramohan Ramasamy, Madan Khurana, Indra Mani, Prerna Kumar, Kailash Pandey
Review
Oncology
Vishakha Anand Pawar, Anuradha Tyagi, Chaitenya Verma, Kanti Prakash Sharma, Sekhu Ansari, Indra Mani, Shailesh Kumar Srivastva, Pradeep Kumar Shukla, Antresh Kumar, Vinay Kumar
Summary: Drug repurposing refers to the use of drugs that have been approved or assessed for different diseases than their original indications. Recent research focuses on patient-centered approaches and explores the mechanisms of pathological abnormalities, and the availability of information on repurposed drugs increases the chances of approval in clinical trials. Repurposed drug immunotherapy has been successful in treating various diseases.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2023)
Article
Immunology
Surendra Kumar Sharma, Rohini Sharma, Binit Kumar Singh, Vishwanath Upadhyay, Indra Mani, Madhavi Tripathi, Prahlad Kumar
INDIAN JOURNAL OF MEDICAL RESEARCH
(2019)
Meeting Abstract
Peripheral Vascular Disease
Venkateswara R. Gogulamudi, Indra Mani, Umadevi Subramanian, Kailash N. Pandey
Article
Physiology
Venkateswara Reddy Gogulamudi, Indra Mani, Umadevi Subramanian, Kailash N. Pandey
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2019)
Meeting Abstract
Critical Care Medicine
S. K. Sharma, R. Sharma, B. K. Singh, V. Upadhyay, I. Mani
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2019)
Meeting Abstract
Peripheral Vascular Disease
Venkateswara R. Gogulamudi, Indra Mani, Umadevi Subramanian, Kailash N. Pandey
Meeting Abstract
Biochemistry & Molecular Biology
Indra Mani, Satyabha Tripathi, Renu Garg, Kailash N. Pandey
Meeting Abstract
Biochemistry & Molecular Biology
Indra Mani, Renu Garg, Satyabha Tripathi, Kailash N. Pandey
