Article
Biotechnology & Applied Microbiology
Qiutao Ding, Runsheng Li, Xiaoliang Ren, Lu-Yan Chan, Vincy W. S. Ho, Dongying Xie, Pohao Ye, Zhongying Zhao
Summary: This study uses nanopore DNA sequencing and short reads to investigate the architecture and variation of the 5S rDNA cluster in nematodes. The results show that rDNA units are highly dynamic in terms of sequence and copy number and that the widely used N2 strain is defective in maintaining the stability of the 5S rDNA cluster compared to other wild isolates.
Article
Biochemistry & Molecular Biology
Raphael Gonen, Max Platkov, Ziv Gardos, Sheli Shayir, Inna Levitsky, Marcelo Weinstein, Esther Manor
Summary: This study presents a method for accelerating the detection of chromosome abnormalities in cells using a cheap stain and a modified staining method. The results show that this method has a detection capability for chromosome abnormalities in cells that is more than 10 times higher than traditional methods.
CELL BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Plant Sciences
Amanda S. Santos, Erton M. Almeida, Leonardo P. Felix, Marcelo Guerra
Summary: Research on the chromosomal numbers of 14 species of Linderniaceae revealed that species like Ameroglossum have a chromosome number of 2n = 60 or higher, with similar chromosome structures and distribution of rDNA sites. This suggests a close relationship between representatives of Linderniaceae typical of inselbergs.
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
(2022)
Article
Plant Sciences
Joel M. P. Cordeiro, Mark W. Chase, Eric Hagsater, Erton Mendonca Almeida, Lucas Costa, Gustavo Souza, Felipe Nollet, Leonardo P. Felix
Summary: The Epidendrum nocturnum Jacq. group is taxonomically complex in Brazil, with this study revealing insights into chromosome evolution, species delimitation, and relationships among representatives of the group, including the description of a new species from the Cerrado and Amazon Rainforest habitats. The study also identified different ploidy levels among the species, suggesting recent polyploid origins for the group.
Article
Limnology
LIU Yu, LIU Pengfei, BI Yanhui, ZHOU Zhigang
Summary: The linkage of 5S rRNA gene to 18S-5.8S-25S rRNA gene was reported in some species in Ochrophyta. Dual-color fluorescence in situ hybridization technique revealed the localization and physical unlinkage of rRNA genes in Saccharina japonica, providing insights into the cytogenetics of this kelp species.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2021)
Article
Plant Sciences
Hoda B. M. Ali, Samy A. A. Heiba, Atef A. A. Haiba
Summary: The study aimed to illustrate the DAPI banding patterns in 16 Brassicaceae species with small chromosome size. It was found that plants with small chromosomes tend to have centromeric bands, while larger chromosomes have a higher tendency for interstitial bands.
EGYPTIAN JOURNAL OF BOTANY
(2021)
Article
Cell Biology
Biplab Kumar Bhowmick, Sumita Jha
Summary: This study investigated the chromosomes of ridged gourd and its wild relative using the enzymatic maceration and air-drying (EMA) method, revealing similar banding patterns and 26 chromosomes in each species. This conservation of banding patterns between the species suggests potential revisions to genomic kinships and evolutionary distance, providing valuable data for genome research and breeding strategies.
Article
Cell Biology
Linjiao Wang, Maoyin Sheng, Xueliang Ren
Summary: Physical mapping of 18S and 5S rDNA sites in eight Nicotiana species using double-probe FISH revealed differences in chromosomal distribution among different species. These findings contribute to understanding the genome evolution of the genus Nicotiana and have implications for genetic breeding and germplasm innovation in tobacco.
Article
Fisheries
Vishwamitra Singh Baisvar, Basdeo Kushwaha, Ravindra Kumar, Murali Sanjeev Kumar, Manmohan Pandey, Mahender Singh
Summary: The chromosomal characteristics of Clarias magur were examined using conventional and molecular cytogenetic tools, revealing the diploid chromosome number, karyotype, and specific features. These findings provided valuable markers for chromosome identification and gene locations, aiding in the construction of the physical map of this species' genome.
JOURNAL OF APPLIED ICHTHYOLOGY
(2021)
Article
Plant Sciences
Youcef Bougoutaia, Teresa Garnatje, Meriem Kaid-Harche, Sonja Siljak-Yakovlev, Joan Valles
Summary: Argania spinosa, a monotypic genus endemic to southern Morocco, is an important plant with high pastoral value, traditional uses, and industrial significance. This study presents the nuclear DNA content and chromosomal characteristics of this species, contributing to its molecular cytogenetics. The small genome size and unique chromosome number observed suggest a dysploidy process in the Sapotaceae family.
Article
Agriculture, Dairy & Animal Science
Maria Siles-Vallejos, Olga Bracamonte-Guevara, Marlon Garcia-Paitan, Alberto Lopez-Sotomayor
Summary: Molecular cytogenetic studies on Physalis peruviana (Golden berry) revealed that the species may be polyploid, with no differences in the number of 5S rDNA loci between populations from different regions, indicating that they may not be different ecotypes.
SCIENTIA AGROPECUARIA
(2021)
Article
Genetics & Heredity
Neeru Kamboj, Anita Bhatnagar, Abhay Singh Yadav
Summary: The study investigated and compared the karyotypes of three species of Puntius in aquatic ecosystems of Haryana, India. It was found that all three species had a diploid chromosome number of 50 and exhibited constitutive heterochromatin in specific regions of the chromosomes. Additionally, variations in chromosome structure were observed among the three species.
CYTOLOGY AND GENETICS
(2021)
Article
Fisheries
Sevgi Unal Karakus, Muhammet Gaffaroglu
Summary: The karyotype and distribution of constitutive heterochromatin and nucleolus organizer regions (NORs) of the Anatolian leuciscine endemic to Lake Beysehir, Squalius anatolicus, were analyzed. The findings showed remarkable chromosome stasis in this leuciscid lineage, as the karyotype pattern and NOR phenotype of S. anatolicus were nearly identical to other Eurasian leuciscine cyprinids analyzed to date.
SU URUNLERI DERGISI
(2021)
Article
Marine & Freshwater Biology
Alexander G. Osinov, Alexander A. Volkov, Nikolai S. Mugue
Summary: The evolutionary history, systematics, and taxonomy of charrs of the genus Salvelinus, particularly the S. alpinus-S. malma species complex, remain confused due to ecological and morphological flexibility of the group and historical hybridization. Analysis of phylogenetic relationships and introgressive hybridization between species revealed complexities due to incomplete lineage sorting and hybridization, suggesting the important role of introgressive hybridization in the evolutionary history of charrs and the appearance of high diversity in morphology, ecology, and taxonomy.
Article
Plant Sciences
Olga Yu. Yurkevich, Tatiana E. Samatadze, Inessa Yu. Selyutina, Svetlana I. Romashkina, Svyatoslav A. Zoshchuk, Alexandra V. Amosova, Olga V. Muravenko
Summary: Research on the genus Hedysarum L. (Fabaceae: Hedysareae) is still incomplete, with systematic knowledge lacking. This study constructed an integrated distribution map for eight Hedysarum species in Eurasia and conducted cytogenomic characterization on twenty accessions to evaluate genomic diversity and relationships within the section. The study identified four main karyotype groups based on chromosomal markers, highlighting the potential for taxonomic studies within this section.
Article
Plant Sciences
Alexandra V. Amosova, Lilit Ghukasyan, Olga Yu. Yurkevich, Nadezhda L. Bolsheva, Tatiana E. Samatadze, Svyatoslav A. Zoshchuk, Olga V. Muravenko
Summary: The genus Deschampsia P. Beauv. includes a group of polymorphic species that are highly tolerant to stressful environmental conditions. In this study, CON/COM repeats were identified and classified in genomes of different species within the genus, showing high homology but potential divergence due to single-nucleotide alterations. Chromosome mapping demonstrated variability in localization of cytogenetic markers, aiding in the identification of chromosomal rearrangements. This research provides valuable insight for genetic and phylogenetic studies within the Deschampsia genus.
Article
Marine & Freshwater Biology
Alexander G. Osinov, Alexander A. Volkov, Dimitri A. Pavlov
Summary: This study analyzed microsatellite loci, sequences of the mtDNA control region, and morphological characteristics to determine the number of sympatric forms of Arctic charr in three lakes in the Taimyr region. The results showed that there are eight reproductively isolated forms in Lama and Kapchuk Lakes, and at least three forms in Lake Sobach'e. The origin of these forms is associated with the hybridization of the European and Siberian lineages.
Article
Plant Sciences
Olga Yu. Yurkevich, Tatiana E. Samatadze, Inessa Yu. Selyutina, Natalia A. Suprun, Svetlana N. Suslina, Svyatoslav A. Zoshchuk, Alexandra V. Amosova, Olga V. Muravenko
Summary: This study used repeatome analysis to reveal the species relationships within the sect. Multicaulia and assess genome diversity through chromosome markers. The results showed a close genomic relationship among the six studied species, confirming the subspecies status of H. setigerum and the validity of combining the sect. Multicaulia and Subacaulia. The findings provide important insights into the taxonomy and phylogeny of the sect. Multicaulia.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Genetics & Heredity
Alexandra Amosova, Olga Yu Yurkevich, Nadezhda L. Bolsheva, Tatiana E. Samatadze, Svyatoslav A. Zoshchuk, Olga Muravenko
Summary: This study conducted a comparative repeatome analysis of subpolar and polar ecotypes of Deschampsia sukatschewii, revealing the genetic composition and chromosomal distribution patterns of satellite DNA. The findings provide new molecular chromosome markers for Deschampsia species.
Article
Plant Sciences
Nadezhda L. Bolsheva, Nataliya V. Melnikova, Ekaterina M. Dvorianinova, Liudmila N. Mironova, Olga Y. Yurkevich, Alexandra V. Amosova, George S. Krasnov, Alexey A. Dmitriev, Olga V. Muravenko
Summary: The phylogenetic relationship between Linum stelleroides and other blue-flowered flax species in the Linaceae family has been controversial. Using genetic sequencing data, this study clarified the issue and found that Linum stelleroides forms a basal branch in the blue-flowered flax clade.
Article
Plant Sciences
Ilya Kirov, Elizaveta Kolganova, Maxim Dudnikov, Olga Yu. Yurkevich, Alexandra V. Amosova, Olga V. Muravenko
Summary: High-copy tandemly organized repeats (TRs), or satellite DNA, are an important and mysterious component of eukaryotic genomes. In this study, we developed NanoTRF, a new python pipeline for the de novo identification of TRs in raw Nanopore sequencing data. NanoTRF can generate informative reports on TR genome abundance, monomer sequence, and length. It also performs annotation of transposable element sequences within or near satDNA arrays, providing insights into the co-evolution of TRs and transposable elements in the genome.
Article
Plant Sciences
Olga Muravenko, Olga Yu Yurkevich, Julia Kalnyuk, Tatiana E. Samatadze, Svyatoslav A. Zoshchuk, Alexandra Amosova
Summary: This study conducted a comparative analysis of the genome organization and chromosome structure of the important species Salvia officinalis and Salvia sclarea. The results revealed interspecific variations in repetitive DNA and identified potential chromosome markers. This research provides valuable insights into the taxonomy and genetic diversity of these two Salvia species.
Article
Plant Sciences
Tatiana E. Samatadze, Olga Yu Yurkevich, Firdaus M. Khazieva, Irina Basalaeva, Elena A. Konyaeva, Alla E. Burova, Svyatoslav A. Zoshchuk, Alexander Morozov, Alexandra Amosova, Olga Muravenko
Summary: Polemonium caeruleum is a valuable medicinal herb with high productivity and active ingredient content in tetraploid plants. The stability of chromosomes and specific characteristics of the plant are significant for raw material identification.
Article
Biochemistry & Molecular Biology
K. S. Vishnyakova, K. Popov, X. Pan, M. Jasko, Y. E. Yegorov
Summary: Excessive presence of hydrophobic molecules can be toxic and disrupt membrane function. Different free fatty acids (FFA) have varying effects on cultured cells, generally leading to lipid accumulation and metabolic shifts.
Article
Engineering, Multidisciplinary
A. A. Volkov, I. A. Volkov, A. G. Plugov, E. V. Kulyabina, O. N. Melkova, G. S. Lavrov, D. V. Bocharova, Ya. I. Alekseev
Summary: Metrological support for DNA sequencing has been developed, with a standard sample of human mitochondrial DNA sequence sequenced using the Sanger method on a domestic capillary electrophoresis genetic analyzer. The results were used to approve the standard sample type and DNA sequence measuring instrument.
MEASUREMENT TECHNIQUES
(2021)