Article
Environmental Sciences
Mohammad Nauman Khan, Chengcheng Fu, Jiaqi Li, Yunpeng Tao, Yanhui Li, Jin Hu, Lingling Chen, Zaid Khan, Honghong Wu, Zhaohu Li
Summary: Salt and drought stress are worldwide environmental issues that affect seed germination and agricultural production. Nanopriming, a new seed technology, shows promise in improving crop tolerance to stress factors. This review provides an overview of nanopriming in enhancing salt and drought tolerance, explores the underlying mechanisms, and highlights the importance of physico-chemical properties of nanomaterials and seed coat anatomy in achieving effective nanopriming.
Article
Biotechnology & Applied Microbiology
Qiutao Ding, Runsheng Li, Xiaoliang Ren, Lu-Yan Chan, Vincy W. S. Ho, Dongying Xie, Pohao Ye, Zhongying Zhao
Summary: This study uses nanopore DNA sequencing and short reads to investigate the architecture and variation of the 5S rDNA cluster in nematodes. The results show that rDNA units are highly dynamic in terms of sequence and copy number and that the widely used N2 strain is defective in maintaining the stability of the 5S rDNA cluster compared to other wild isolates.
Article
Biochemistry & Molecular Biology
Raphael Gonen, Max Platkov, Ziv Gardos, Sheli Shayir, Inna Levitsky, Marcelo Weinstein, Esther Manor
Summary: This study presents a method for accelerating the detection of chromosome abnormalities in cells using a cheap stain and a modified staining method. The results show that this method has a detection capability for chromosome abnormalities in cells that is more than 10 times higher than traditional methods.
CELL BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Plant Sciences
Amanda S. Santos, Erton M. Almeida, Leonardo P. Felix, Marcelo Guerra
Summary: Research on the chromosomal numbers of 14 species of Linderniaceae revealed that species like Ameroglossum have a chromosome number of 2n = 60 or higher, with similar chromosome structures and distribution of rDNA sites. This suggests a close relationship between representatives of Linderniaceae typical of inselbergs.
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
(2022)
Article
Plant Sciences
Joel M. P. Cordeiro, Mark W. Chase, Eric Hagsater, Erton Mendonca Almeida, Lucas Costa, Gustavo Souza, Felipe Nollet, Leonardo P. Felix
Summary: The Epidendrum nocturnum Jacq. group is taxonomically complex in Brazil, with this study revealing insights into chromosome evolution, species delimitation, and relationships among representatives of the group, including the description of a new species from the Cerrado and Amazon Rainforest habitats. The study also identified different ploidy levels among the species, suggesting recent polyploid origins for the group.
Article
Limnology
LIU Yu, LIU Pengfei, BI Yanhui, ZHOU Zhigang
Summary: The linkage of 5S rRNA gene to 18S-5.8S-25S rRNA gene was reported in some species in Ochrophyta. Dual-color fluorescence in situ hybridization technique revealed the localization and physical unlinkage of rRNA genes in Saccharina japonica, providing insights into the cytogenetics of this kelp species.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2021)
Article
Plant Sciences
Hoda B. M. Ali, Samy A. A. Heiba, Atef A. A. Haiba
Summary: The study aimed to illustrate the DAPI banding patterns in 16 Brassicaceae species with small chromosome size. It was found that plants with small chromosomes tend to have centromeric bands, while larger chromosomes have a higher tendency for interstitial bands.
EGYPTIAN JOURNAL OF BOTANY
(2021)
Article
Cell Biology
Biplab Kumar Bhowmick, Sumita Jha
Summary: This study investigated the chromosomes of ridged gourd and its wild relative using the enzymatic maceration and air-drying (EMA) method, revealing similar banding patterns and 26 chromosomes in each species. This conservation of banding patterns between the species suggests potential revisions to genomic kinships and evolutionary distance, providing valuable data for genome research and breeding strategies.
Article
Cell Biology
Linjiao Wang, Maoyin Sheng, Xueliang Ren
Summary: Physical mapping of 18S and 5S rDNA sites in eight Nicotiana species using double-probe FISH revealed differences in chromosomal distribution among different species. These findings contribute to understanding the genome evolution of the genus Nicotiana and have implications for genetic breeding and germplasm innovation in tobacco.
Article
Plant Sciences
Ahmed M. Faried, Zeinab A. El-Karemy, Hasnaa A. Hosni, Sara M. Saleh, Mostafa Aboulela
Summary: The anatomical structure of seed coats of 13 Egyptian taxa belonging to three tribes of Fabaceae-Papilionoideae was investigated. Variations in seed-coat characteristics were observed among the studied taxa. The analysis of seed anatomical characteristics helps in understanding the phenetic relationships among taxa at different levels.
BRAZILIAN JOURNAL OF BOTANY
(2022)
Article
Plant Sciences
Youcef Bougoutaia, Teresa Garnatje, Meriem Kaid-Harche, Sonja Siljak-Yakovlev, Joan Valles
Summary: Argania spinosa, a monotypic genus endemic to southern Morocco, is an important plant with high pastoral value, traditional uses, and industrial significance. This study presents the nuclear DNA content and chromosomal characteristics of this species, contributing to its molecular cytogenetics. The small genome size and unique chromosome number observed suggest a dysploidy process in the Sapotaceae family.
Article
Plant Sciences
Waheed Arshad, Teresa Lenser, Per K. I. Wilhelmsson, Jake O. Chandler, Tina Steinbrecher, Federica Marone, Marta Perez, Margaret E. Collinson, Wolfgang Stuppy, Stefan A. Rensing, Gunter Theissen, Gerhard Leubner-Metzger
Summary: A comparative imaging and transcriptome analysis was conducted to investigate morph-specific developmental differences in Aethionema arabicum, a Brassicaceae model for diaspore dimorphism, revealing two distinct morphologies in seed coat development with mucilaginous and non-mucilaginous diaspores. The research highlights the importance of studying epidermal cell differentiation and mucilage biosynthesis control mechanisms using Ae. arabicum as a model system.
Article
Agriculture, Dairy & Animal Science
Maria Siles-Vallejos, Olga Bracamonte-Guevara, Marlon Garcia-Paitan, Alberto Lopez-Sotomayor
Summary: Molecular cytogenetic studies on Physalis peruviana (Golden berry) revealed that the species may be polyploid, with no differences in the number of 5S rDNA loci between populations from different regions, indicating that they may not be different ecotypes.
SCIENTIA AGROPECUARIA
(2021)
Article
Biochemistry & Molecular Biology
Cheng Liu, Xianlian Chen, Wubin Wang, Xinyang Hu, Wei Han, Qingyuan He, Hongyan Yang, Shihua Xiang, Junyi Gai
Summary: In this study, an improved wild soybean chromosome segment substitution line (CSSL) population, SojaCSSLP5, was developed, which was genotyped through whole-genome resequencing to identify genetic changes from wild soybean to cultivated soybean. Using the SNPLDB-map, several candidate genes related to seed-coat color and days to flowering were identified and verified, demonstrating the efficiency of the CSSL population in identifying candidate wild vs. cultivated gene-alleles. The integration of parental DNA and RNA sequencing data provided a reliable platform for further research on genetic changes between wild and cultivated soybean.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
E. Boyko, E. Novozhilova
Summary: We studied the seed coat structure of 52 species from 31 genera of the Heliantheae tribe of the Asteraceae family. The seed coat structure was observed using scanning electron microscopy. The study revealed that the species of the tribe have distinct sculptured or smooth seed coat, determined by the exotesta cells. There are various types and subtypes of exotesta cell structures due to the thickening of cell walls. The seed coat surface sculpture is diverse among the species in the tribe, making it difficult to identify common characteristics within the tribe, as well as its subtribes and genera. The division of the Heliantheae tribe into subtribes is not supported by our study. The conservative nature of the testa cell structure can be used in taxonomy, phylogenetics, and determining the homogeneity of taxa within the tribe.
Article
Endocrinology & Metabolism
Maria Savvateeva, Anna Kudryavtseva, Elena Lukyanova, Anastasiya Kobelyatskaya, Vladislav Pavlov, Maria Fedorova, Elena Pudova, Zulfiya Guvatova, Dmitry Kalinin, Alexander Golovyuk, Elizaveta Bulavkina, Irina Katunina, George Krasnov, Anastasiya Snezhkina
Summary: This study analyzed somatic mutation profiles in HNPGLs and found that most patients had germline and somatic variants in paraganglioma susceptibility genes or related genes. The study also identified six significantly mutated genes and four mutational signatures associated with HNPGLs. These findings provide important insights into the molecular mechanisms involved in the development of HNPGLs.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Gastroenterology & Hepatology
Roman Maslennikov, Vladimir Ivashkin, Irina Efremova, Elena Poluektova, Anna Kudryavtseva, George Krasnov
Summary: This study found a relationship between small intestinal bacterial overgrowth (SIBO) and gut dysbiosis in patients with cirrhosis. Patients with SIBO had higher levels of Firmicutes and Fusobacteria, and lower levels of Bacteroidetes compared to patients without SIBO. However, gut dysbiosis and SIBO are likely independent disorders in cirrhosis.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Plant Sciences
Olga Yu. Yurkevich, Tatiana E. Samatadze, Inessa Yu. Selyutina, Natalia A. Suprun, Svetlana N. Suslina, Svyatoslav A. Zoshchuk, Alexandra V. Amosova, Olga V. Muravenko
Summary: This study used repeatome analysis to reveal the species relationships within the sect. Multicaulia and assess genome diversity through chromosome markers. The results showed a close genomic relationship among the six studied species, confirming the subspecies status of H. setigerum and the validity of combining the sect. Multicaulia and Subacaulia. The findings provide important insights into the taxonomy and phylogeny of the sect. Multicaulia.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Genetics & Heredity
Alexandra Amosova, Olga Yu Yurkevich, Nadezhda L. Bolsheva, Tatiana E. Samatadze, Svyatoslav A. Zoshchuk, Olga Muravenko
Summary: This study conducted a comparative repeatome analysis of subpolar and polar ecotypes of Deschampsia sukatschewii, revealing the genetic composition and chromosomal distribution patterns of satellite DNA. The findings provide new molecular chromosome markers for Deschampsia species.
Article
Plant Sciences
Nadezhda L. Bolsheva, Nataliya V. Melnikova, Ekaterina M. Dvorianinova, Liudmila N. Mironova, Olga Y. Yurkevich, Alexandra V. Amosova, George S. Krasnov, Alexey A. Dmitriev, Olga V. Muravenko
Summary: The phylogenetic relationship between Linum stelleroides and other blue-flowered flax species in the Linaceae family has been controversial. Using genetic sequencing data, this study clarified the issue and found that Linum stelleroides forms a basal branch in the blue-flowered flax clade.
Article
Plant Sciences
Tatiana E. Samatadze, Olga Yu Yurkevich, Firdaus M. Khazieva, Irina Basalaeva, Elena A. Konyaeva, Alla E. Burova, Svyatoslav A. Zoshchuk, Alexander Morozov, Alexandra Amosova, Olga Muravenko
Summary: Polemonium caeruleum is a valuable medicinal herb with high productivity and active ingredient content in tetraploid plants. The stability of chromosomes and specific characteristics of the plant are significant for raw material identification.
Article
Biochemistry & Molecular Biology
Anastasiya Snezhkina, Maria Fedorova, Anastasiya Kobelyatskaya, Daria Markova, Margarita Lantsova, Anna Ikonnikova, Marina Emelyanova, Dmitry Kalinin, Elena Pudova, Nataliya Melnikova, Alexey Dmitriev, George Krasnov, Vladislav Pavlov, Anna Kudryavtseva
Summary: In this study, a high frequency missense variant p.H102R in the SDHD gene was found in an extended cohort of Russian HNPGL patients using whole-exome sequencing. The variant was associated with somatic loss of the gene wild-type allele and evident LOH at the 11p15.5 region and potential loss of the whole of chromosome 11. These findings help with understanding the mechanism of tumorigenesis and are important for the development of cost-effective genetic screening programs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Vladimir Ivashkin, Oleg Shifrin, Roman Maslennikov, Elena Poluektova, Alexander Korolev, Anna Kudryavtseva, George Krasnov, Nona Benuni, Giovanni Barbara
Summary: This study investigated the changes in the fecal microbiome of patients with symptomatic uncomplicated diverticular disease (SUDD) after repeated monthly treatment with rifaximin and found that rifaximin effectively decreased abdominal pain in these patients. The study also showed that rifaximin increased the abundance of beneficial microbial elements in the gut.
BMC GASTROENTEROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anastasiya Snezhkina, Vladislav Pavlov, Maria Fedorova, Dmitry Kalinin, Elena Pudova, Anastasiya Kobelyatskaya, Ildar Bakhtogarimov, George Krasnov, Anna Kudryavtseva
Summary: Malignant middle ear paraganglioma (MEPGL) is a rare neuroendocrine tumor. A case of malignant MEPGL was studied using comprehensive genetic analysis. A germline pathogenic mutation in the SDHB gene was identified, along with five novel somatic variants. The analysis showed the presence of specific mutation signatures and revealed the importance of SDHB mutation combined with loss of heterozygosity in tumor initiation and progression. These findings enhance our understanding of the molecular genetic mechanisms associated with this rare tumor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Olga N. Ivanova, George S. Krasnov, Anastasiya V. Snezhkina, Anna V. Kudryavtseva, Vyacheslav S. Fedorov, Natalia F. Zakirova, Michail V. Golikov, Sergey N. Kochetkov, Birke Bartosch, Vladimir T. Valuev-Elliston, Alexander V. Ivanov
Summary: ROS play a major role in cell regulation, and their increase is associated with the development of various pathologies. This study analyzed gene expression of redox systems and related metabolic processes in hepatoma cells and liver progenitor cell lines, shedding light on the redox biology of viral hepatitis and the influence of laboratory models.
Article
Biochemistry & Molecular Biology
Anastasiya A. Kobelyatskaya, Elena A. Pudova, Irina V. Katunina, Anastasiya V. Snezhkina, Maria S. Fedorova, Vladislav S. Pavlov, Anastasiya O. Kotelnikova, Kirill M. Nyushko, Boris Y. Alekseev, George S. Krasnov, Anna V. Kudryavtseva
Summary: This study used RNA-Seq profiling to analyze localized and locally advanced prostate cancer tissue samples from Russian patients. The study identified different transcriptome profiles and affected biological processes within high-risk and intermediate risk groups, providing potential therapeutic targets and prognostic markers for the considered categories of prostate cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Aleksandra Kovaleva, Elena Poluektova, Roman Maslennikov, Anna Karchevskaya, Oleg Shifrin, Andrey Kiryukhin, Aleksandr Tertychnyy, Leonid Kovalev, Marina Kovaleva, Olga Lobanova, Anna Kudryavtseva, George Krasnov, Maria Fedorova, Vladimir Ivashkin
Summary: The study evaluated the effects of rebamipide in patients with irritable bowel syndrome, showing that it significantly reduces digestive symptoms and improves intestinal barrier status. Rebamipide is as effective as trimebutine in treating these conditions.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Gastroenterology & Hepatology
Aleksandra Kovaleva, Elena Poluektova, Roman Maslennikov, Oxana Zolnikova, Oleg Shifrin, Anna Kudryavtseva, George Krasnov, Maria Fedorova, Anna Karchevskaya, Vladimir Ivashkin
Summary: This study investigated the composition and metabolic activity of the gut microbiota in patients with diarrhea-predominant irritable bowel syndrome combined with functional dyspepsia (I + D). The study found significant differences in the abundance of certain gut bacteria as well as higher levels of short-chain fatty acids (SCFAs) in the feces of patients compared to healthy controls. Additionally, the levels of certain fatty acids in the feces correlated with the severity of the symptoms.
GASTROINTESTINAL DISORDERS
(2023)
Article
Biochemistry & Molecular Biology
Elena A. Pudova, Anastasiya A. Kobelyatskaya, Irina V. Katunina, Anastasiya V. Snezhkina, Maria S. Fedorova, Vladislav S. Pavlov, Ildar R. Bakhtogarimov, Margarita S. Lantsova, Sergey P. Kokin, Kirill M. Nyushko, Boris Ya. Alekseev, Dmitry V. Kalinin, Nataliya V. Melnikova, Alexey A. Dmitriev, George S. Krasnov, Anna V. Kudryavtseva
Summary: Radical prostatectomy is the gold standard treatment for prostate cancer, but complete cure is not always achieved and recurrence is common. The current methods for assessing prognosis and choosing treatment strategies in PCa patients are not sufficient and new markers are needed. This study used RNA-Seq to identify potential prognostic markers at the gene expression and miRNA levels associated with cancer aggressiveness. The expression of candidate markers was validated and the predictive value of different marker combinations was analyzed using machine learning algorithms, with the CST2 + OCLN + pT model showing the highest potential (AUC = 0.863) based on CatBoost Classifier algorithm.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Zulfiya G. Guvatova, Anastasiya A. Kobelyatskaya, Elena A. Pudova, Irina V. Tarasova, Anna V. Kudryavtseva, Olga N. Tkacheva, Irina D. Strazhesko, Alexey A. Moskalev
Summary: This study estimated the epigenetic age of long-lived individuals and found that their epigenetic age was significantly lower than their chronological age, indicating a slowing of aging. However, there were no significant differences in epigenetic age between healthy long-lived individuals and those with frailty, and no differences in epigenetic age were observed based on sex. The study emphasizes the need for further research on the epigenetic status of centenarians.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
