Article
Medicine, Research & Experimental
Adriana Sanna, Bengt Phung, Shamik Mitra, Martin Lauss, Jiyeon Choi, Tongwu Zhang, Ching-Ni Njauw, Eugenia Cordero, Katja Harbst, Frida Rosengren, Rita Cabrita, Iva Johansson, Karolin Isaksson, Christian Ingvar, Ana Carneiro, Kevin Brown, Hensin Tsao, My Andersson, Kristian Pietras, Goran Jonsson
Summary: Cellular stress contributes to the phenotype switching of melanoma cells, and this is associated with the loss of specific gene expression and increased expression of mesenchymal markers. The loss of microphthalmia-associated transcription factor (MITF) and SRY-box transcription factor 10 (SOX10) is related to this process, and their loss is associated with promoter hypermethylation and poor patient survival.
Article
Developmental Biology
Karen K. Deal, Jennifer C. Rosebrock, Angela M. Eeds, Jean-Marc L. DeKeyser, Melissa A. Musser, Sara J. Ireland, Aaron A. May-Zhang, Dennis P. Buehler, E. Michelle Southard-Smith
Summary: The study explores the diversity of neural crest derivatives in embryonic development by utilizing Cre-LoxP lineage tracing and identifying Sox10 as an essential transcription factor. New Sox10-cre BAC transgenes offer a comprehensive expression profile of Sox10, revealing novel sites of expression and a narrow temporal window of expression in mesenchymal derivatives during embryogenesis. The transgenic lines provide tools for refined lineage analysis of neural crest derivatives and glandular tissues reliant on Sox10.
DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Werend Boesmans, Amelia Nash, Kinga R. Tasnady, Wendy Yang, Lincon A. Stamp, Marlene M. Hao
Summary: Enteric glia are support cells of the enteric nervous system that play a vital role in regulating gut functions and maintaining homeostasis. Their plasticity and heterogeneity make them neural precursors with the potential for neurogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Zhixin Li, Elly Sau-Wai Ngan
Summary: With the rapid development of single-cell sequencing technologies, the use of single-cell multimodal sequencing has become a powerful strategy in discovering rare cells and understanding biological processes. This article summarizes recent findings in single-cell multimodal sequencing, particularly focusing on single-cell RNA sequencing (scRNA-seq) in the enteric nervous system (ENS). The studies have revealed the heterogeneity of ENS cells at different developmental stages and identified novel cell types. The application of single-cell multimodal omics techniques in profiling disease mechanisms and cellular drug responses is expected to revolutionize biomedical research.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Cell & Tissue Engineering
Rhian Stavely, Sukhada Bhave, Wing Lam N. Ho, Minhal Ahmed, Weikang Pan, Ahmed A. Rahman, Jessica Ulloa, Nicole Bousquet, Meredith Omer, Richard Guyer, Nandor Nagy, Allan M. Goldstein, Ryo Hotta
Summary: The interaction between ENSCs and EMCs plays a crucial role in the normal development of the enteric nervous system, with potential implications for treating Hirschsprung disease. EMCs enhance the expansion and neurosphere-forming ability of ENCDCs through paracrine factors, leading to functional neurons and successful transplantation in the HSCR model.
Review
Biochemistry & Molecular Biology
Richard A. Guyer, Jessica L. Mueller, Allan M. Goldstein
Summary: With the advancement of technology, single-cell sequencing has become a common tool to analyze cellular diversity and reveal cellular diversity within organs. The application of these methods in the study of the enteric nervous system has provided abundant data and biological insights. As technology continues to evolve, these methods may become commonplace in enteric nervous system research and diagnostic testing.
Article
Developmental Biology
Rhian Stavely, Ryo Hotta, Richard A. Guyer, Nicole Picard, Ahmed A. Rahman, Meredith Omer, Adam Soos, Emoke Szocs, Jessica Mueller, Allan M. Goldstein, Nandor Nagy
Summary: The development of the enteric nervous system is dependent on the colonization of the intestine by enteric neural crest-derived cells (ENCDCs). ENCDCs at the wavefront, which are highly migratory and proliferative, exhibit distinct molecular characteristics compared to trailing ENCDCs. These wavefront ENCDCs show altered expression of genes associated with migration and lack expression of genes related to neuronal or glial maturation.
Article
Developmental Biology
Nandor Nagy, Tamas Kovacs, Rhian Stavely, Viktoria Halasy, Adam Soos, Emoke Szocs, Ryo Hotta, Hannah Graham, Allan M. Goldstein
Summary: The migration and proliferation of ENCCs are crucial for ENS formation, with the ceca playing an important role in ENS development. The non-canonical Wnt signaling pathway regulates ENCC differentiation by inhibiting enteric neuronal differentiation.
Article
Pediatrics
Naho Fujiwara, Katsumi Miyahara, Nana Nakazawa-Tanaka, Chihiro Akazawa, Atsuyuki Yamataka
Summary: This study investigated the migration and differentiation of transplanted stem cells in the colon of patients with Hirschsprung disease. The results showed a decrease in cellular migration and differentiation in the patient's colon compared to the control group. This highlights the importance of the neuronal environment in the recipient gut for enteric nervous system development.
PEDIATRIC SURGERY INTERNATIONAL
(2022)
Article
Genetics & Heredity
Sumantra Chatterjee, Lauren E. Fries, Or Yaacov, Nan Hu, Hanna E. Berk-Rauch, Aravinda Chakravarti
Summary: Hirschsprung disease is associated with deficiency of the RET receptor tyrosine kinase, resulting in loss of cells of the enteric nervous system during fetal gut development. This study demonstrates that variants in RET enhancers specifically alter the human fetal gut development program by decreasing gene expression of RET and other genes involved in Hirschsprung disease, leading to differential expression of genes across neuronal and mesenchymal functions. The findings suggest that genes reducing RET proliferative activity can potentially cause Hirschsprung disease.
Article
Cell Biology
Yuuki Shikaya, Yuta Takase, Ryosuke Tadokoro, Ryo Nakamura, Masafumi Inaba, Yoshiko Takahashi
Summary: Gut peristaltic movements are crucial for effective transportation and digestion/absorption of ingested materials. The cellular functions underlying these movements along the gut axis during development are largely unexplored. By studying chicken embryonic gut, we found that peristaltic movements start from the posterior duodenum and become confined to specific regions during development. Manipulations of the enteric nervous system disrupted the distribution patterns of peristaltic waves and affected the transportation of ingested material. We also discovered a functional coupling between the hindgut and cloaca.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tamas Kovacs, Viktoria Halasy, Csongor Petho, Emoke Szocs, Adam Soos, David Dora, Pascal de Santa Barbara, Sandrine Faure, Rhian Stavely, Allan M. Goldstein, Nandor Nagy, Nicolas Pilon
Summary: The enteric nervous system (ENS) is derived from vagal neural crest cells and plays a crucial role in gastrointestinal development. This study shows that the avian ceca, located at the junction of the midgut and hindgut, are necessary for hindgut ENS development. It also highlights the importance of bone morphogenetic protein-4 (BMP4) and glial cell line-derived neurotrophic factor (GDNF) signaling pathways in the migration of enteric neural crest-derived cells (ENCDC) and the formation of enteric ganglia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
Viktoria Halasy, Emoke Szocs, Adam Soos, Tamas Kovacs, Nora Pecsenye-Fejszak, Ryo Hotta, Allan M. Goldstein, Nandor Nagy
Summary: The enteric nervous system (ENS) and peripheral ganglia provide innervation to the gastrointestinal tract, but the molecular mechanisms controlling extrinsic nerve fiber growth are not well understood. This study investigates the role of CXCR4 and CXCL12 in the development of colorectal innervation. It is found that CXCR4 is expressed in nerve fibers arising from the nerve of Remak (NoR) and the pelvic plexus, while CXCL12 is localized to the hindgut and enteric ganglia. Overexpression of CXCL12 enhances axonal projections to the gut from the NoR, while CXCR4 inhibition disrupts nerve fiber extension.
Article
Genetics & Heredity
Wendy Yang, Szu-Chieh Chen, Tse-En Wang, Pei-Shiue Tsai, Jeng-Chang Chen, Pei-Lung Chen
Summary: A study identified an L1CAM gene variation associated with mild hydrocephalus and HSCR, and introduced a similar variation into mice using the CRISPR-EZ method. Different phenotypes were observed in the mice, with no prominent hydrocephalus or HSCR-like symptoms, but male infertility was noticed after three generations. Shorter L1cam isoform proteins were found to be overexpressed in the intestine of L1cam knockdown mice, indicating their potential role in protecting against HSCR.
Article
Pediatrics
Naho Fujiwara, Katsumi Miyahara, Nana Nakazawa-Tanaka, Yoshie Oishi, Chihiro Akazawa, Norihiro Tada, Atsuyuki Yamataka
Summary: This study demonstrated the importance of an appropriate host environment for the colonization of the gut, and further investigations are needed to improve the results of this model.
PEDIATRIC SURGERY INTERNATIONAL
(2023)
Article
Developmental Biology
Kylie M. Georgas, Jane Armstrong, Janet R. Keast, Christine E. Larkins, Kirk M. McHugh, E. Michelle Southard-Smith, Martin J. Cohn, Ekatherina Batourina, Hanbin Dan, Kerry Schneider, Dennis P. Buehler, Carrie B. Wiese, Jane Brennan, Jamie A. Davies, Simon D. Harding, Richard A. Baldock, Melissa H. Little, Chad M. Vezina, Cathy Mendelsohn
Article
Developmental Biology
Wing Hei Chan, David G. Gonsalvez, Heather M. Young, E. Michelle Southard-Smith, Kylie N. Cane, Colin R. Anderson
DEVELOPMENTAL NEUROBIOLOGY
(2016)
Article
Gastroenterology & Hepatology
Jumpei Kondo, Anne E. Powell, Yang Wang, Melissa A. Musser, E. Michelle Southard-Smith, Jeffrey L. Franklin, Robert J. Coffey
Article
Cell Biology
Christopher Dravis, Benjamin T. Spike, J. Chuck Harrell, Claire Johns, Christy L. Trejo, E. Michelle Southard-Smith, Charles M. Perou, Geoffrey M. Wahl
Article
Developmental Biology
Nadege Bondurand, E. Michelle Southard-Smith
DEVELOPMENTAL BIOLOGY
(2016)
Article
Multidisciplinary Sciences
Subhash Kulkarni, Maria-Adelaide Micci, Jenna Leser, Changsik Shin, Shiue-Cheng Tang, Ya-Yuan Fu, Liansheng Liu, Qian Li, Monalee Saha, Cuiping Li, Grigori Enikolopov, Laren Becker, Nikolai Rakhilin, Michael Anderson, Xiling Shen, Xinzhong Dong, Manish J. Butte, Hongjun Song, E. Michelle Southard-Smith, Raj P. Kapur, Milena Bogunovic, Pankaj J. Pasricha
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Neurosciences
K. Elaine Ritter, Zunyi Wang, Chad M. Vezina, Dale E. Bjorling, E. Michelle Southard-Smith
FRONTIERS IN NEUROSCIENCE
(2017)
Article
Neurosciences
K. Elaine Ritter, E. Michelle Southard-Smith
FRONTIERS IN NEUROSCIENCE
(2017)
Article
Multidisciplinary Sciences
Aaron A. May-Zhang, Karen K. Deal, E. Michelle Southard-Smith
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Developmental Biology
Melissa A. Musser, E. Michelle Southard-Smith
DEVELOPMENTAL BIOLOGY
(2013)
Article
Neurosciences
Constance A. Rich, Surangi N. Perera, Jacqueline Andratschke, C. Claus Stolt, Dennis P. Buehler, E. Michelle Southard-Smith, Michael Wegner, Stefan Britsch, Clare V. H. Baker
Article
Multidisciplinary Sciences
Keiko Ikeda, Masanori Takahashi, Shigeru Sato, Hiroyuki Igarashi, Toru Ishizuka, Hiromu Yawo, Satoru Arata, E. Michelle Southard-Smith, Kiyoshi Kawakami, Hiroshi Onimaru
Article
Developmental Biology
Carrie B. Wieser, Karen K. Deal, Sara J. Ireland, V. Ashley Cantrell, E. Michelle Southard-Smith
DEVELOPMENTAL BIOLOGY
(2017)
Article
Physiology
Kyle A. Wegner, Lisa L. Abler, Steven R. Oakes, Guneet S. Mehta, K. Elaine Ritter, Warren G. Hill, Bernadette M. Zwaans, Laura E. Lamb, Zunyi Wang, Dale E. Bjorling, William A. Ricke, Jill Macoska, Paul C. Marker, E. Michelle Southard-Smith, Kevin W. Eliceiri, Chad M. Vezina
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2018)
Article
Gastroenterology & Hepatology
Melissa A. Musser, Hernan Correa, E. Michelle Southard-Smith
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2015)