Review
Anatomy & Morphology
Matias Hidalgo-Sanchez, Abraham Andreu-Cervera, Sergio Villa-Carballar, Diego Echevarria
Summary: A crucial event in the development of the central nervous system is the subdivision of the neural tube to form neuromeres, which are genetic units separated by constrictions. The narrower portions of the neural tube are responsible for relevant cellular and molecular processes, such as gene expression and fate specification. The midbrain-hindbrain boundary is a model for studying the specification of these two brain subdivisions, and the interactions between them lead to the formation of specific morphogenes.
FRONTIERS IN NEUROANATOMY
(2022)
Article
Biochemistry & Molecular Biology
Hugo Cruces-Solis, Olga Babaev, Heba Ali, Carolina Piletti Chatain, Vasyl Mykytiuk, Nursen Balekoglu, Sally Wenger, Dilja Krueger-Burg
Summary: This study investigates how the deletion of Neuroligin-2 in mice alters communication between key brain regions involved in mediating defensive behaviors. The study found that Neuroligin-2 knockout resulted in increased LFP power in the vHPC and abnormal modulation of theta frequency oscillations in the vHPC-mPFC-BLA circuit. Additionally, the deletion of Neuroligin-2 increased beta and gamma frequency synchrony across the network and was associated with increased center avoidance behavior.
Article
Immunology
Wenjie Wang, Luyao Liu, Xiaoying Hui, Ying Wang, Wenjing Ying, Qinhua Zhou, Jia Hou, Mi Yang, Bijun Sun, Jinqiao Sun, Xiaochuan Wang
Summary: This study reported a case of a patient with a STAT3 gain-of-function mutation, where tocilizumab therapy proved effective in resolving clinical issues. Gene sequencing can be used for diagnosis and treatment selection in early-onset refractory or multiorgan immune dysregulation diseases.
Review
Plant Sciences
Lin Yue, Xinxin Pei, Fanjiang Kong, Lin Zhao, Xiaoya Lin
Summary: Soybean (Glycine max) is a key crop for protein and oil production. The bZIP transcription factors in soybean play important roles in various regulatory pathways, such as yield improvement, stress response, environmental signaling, and carbon-nitrogen balance. This review provides a comprehensive understanding of the soybean bZIP family, including its members and classification, and offers valuable insights for future research on bZIP transcription factors and genetic resources for soybean breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Ophthalmology
Sriganesh Ramachandra Rao, Steven J. Fliesler
Summary: This study presents a simple, inexpensive, and rapid strategy for genotype-free identification of ectopic leakiness in cell type-specific Cre recombinase-mediated gene editing. By using a custom-designed 3D blue LED light box, any presumed specific Cre mouse line with ectopic or nonspecific Cre recombinase expression can be easily and quickly detected without the need for genotyping or causing distress to the animals.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Long-Yi Chan, Cheng-Chung Chang, Po-Liang Lai, Tomoji Maeda, Horng-Chaung Hsu, Chin-Yu Lin, Shu-Jui Kuo
Summary: This study demonstrates the sustained expression of the cartilage-anabolic factor runx1 in MSCs, which are encapsulated in hyaluronan hydrogel and transplanted through micro-injection. The study shows that the runx1 gene expression has good biosafety characteristics and maintains superior hydration content in the intervertebral disc.
Article
Physiology
Alisha Tromp, Haitao Wang, Thomas E. Hall, Bryan Mowry, Jean Giacomotto
Summary: We introduced Cre/Lox technology in our laboratory for transient and stable/transgenic experiments, but encountered problems such as silencing, mosaicism, and partial recombination. These issues are common among the zebrafish community using the Cre/Lox system. To improve the system, we developed a codon-improved Cre version and established tol2-kit compatible vectors to facilitate the generation of iCre-mRNA or iCre-transgenes.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Cell Biology
Maxim Karagyaur, Daniyar Dyikanov, Pyotr Tyurin-Kuzmin, Stalik Dzhauari, Mariya Skryabina, Maksim Vigovskiy, Alexandra Primak, Natalia Kalinina, Vsevolod Tkachuk
Summary: In this study, a novel Cre/lox71-based system for the controlled transient expression of target genes was developed. The system utilizes the bacteriophage P1 Cre recombinase, which has a highly specific DNA-binding site without endogenous binding sites within mouse or human genomes. By fusing the catalytically inactive form of Cre recombinase with the VP64 transactivation domain, an artificial transcription factor Cre-VP64 was constructed. This transcription factor binds to the lox71 sites within the promoter region of the target gene and enhances its expression. The system demonstrated superior efficiency in regulating transgene expression with high expression level upon induction and low basal activity. This system, along with its modified forms, can serve as an effective tool for the temporary controlled expression of target genes in functional genomic studies and gene therapy approaches.
Article
Physics, Multidisciplinary
Ting-Li Chen, Elizabeth P. P. Chou, Hsieh Fushing
Summary: This research selects collections of major factors embedded within response-versus-covariate dynamics based on information theoretic measurements through Categorical Exploratory Data Analysis (CEDA) computing paradigm, exploring the relevance to Wiener-Granger causality. The selection task identifies a chief collection and several secondary collections, with reliability checks through algorithmic computations.
Article
Genetics & Heredity
Kara Corps, Monica Stanwick, Juliann Rectenwald, Andrew Kruggel, Sarah B. Peters
Summary: The loss of TGF beta receptor II in mice resulted in bone and teeth defects, as well as osteochondral dysplasia throughout the skeleton, potentially leading to breathing difficulties and malnutrition. These abnormalities were primarily caused by skeletal deformities, affecting the mice's ability to sense and absorb food.
Article
Veterinary Sciences
Kumiko Yoshinobu, Masatake Araki, Ayaka Morita, Miyuki Araki, Shun Kokuba, Naomi Nakagata, Kimi Araki
Summary: Feeding method for administering tamoxifen to mice can achieve efficient recombination without causing injury, suitable for whole body and fetal period recombination.
EXPERIMENTAL ANIMALS
(2021)
Review
Oncology
Xinxin Li, Mengzhen Han, Hongwei Zhang, Furong Liu, Yonglong Pan, Jinghan Zhu, Zhibin Liao, Xiaoping Chen, Bixiang Zhang
Summary: Zinc finger proteins, characterized by their different DNA binding motifs, play a significant role in gene regulation. In hepatocellular carcinoma (HCC), zinc finger proteins exhibit diverse functions in tumorigenesis and progression, and they are also of great significance in HCC diagnosis and prognostic evaluation.
BIOMARKER RESEARCH
(2022)
Article
Neurosciences
Nancy V. N. Carullo, Jenna E. Hinds, Jasmin S. Revanna, Jennifer J. Tuscher, Allison J. Bauman, Jeremy J. Day
Summary: Targeted genetic and epigenetic modification is crucial in molecular neuroscience to understand gene regulatory mechanisms underlying complex behaviors. While CRISPR-based systems have revolutionized genome editing, few tools exist for cell-specific CRISPR activation in neurons. This study presents a Cre-dependent CRISPRa system with improved efficiency and specificity, providing a versatile tool for gene regulation in different model systems.
Article
Biochemistry & Molecular Biology
Leonid A. Ilchuk, Nina Stavskaya, Ekaterina A. Varlamova, Alvina Khamidullina, Victor V. Tatarskiy, Vladislav A. Mogila, Ksenia B. Kolbutova, Sergey A. Bogdan, Alexey M. Sheremetov, Alexandr N. Baulin, Irina A. Filatova, Yulia Yu Silaeva, Maxim A. Filatov, Alexandra Bruter
Summary: This article investigates the efficiency of different induction methods on knockout and finds that efficiency is correlated with the concentration of tamoxifen metabolites. Two specific cases of tamoxifen-induced knockout limitations are described. Increasing tamoxifen dosage and activation duration improves knockout rate in the brain but not in the uterus. Induction of knockout during embryonic development is impossible due to the negative effect of tamoxifen on gestation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Guangyu Xu, Yanbo Feng, Han Li, Cong Chen, He Li, Chunmei Wang, Jianguang Chen, Jinghui Sun
Summary: This study explored the molecular mechanism of SA's regulatory effect on the immune function of cyclophosphamide-induced immunosuppressive mice through screening different genes and constructing a transcription factor target gene regulatory network. The results showed that SA alleviated the immunosuppression induced by cyclophosphamide in mice by regulating multiple genes and their transcription factors.
FRONTIERS IN PHARMACOLOGY
(2021)