Editorial Material
Plant Sciences
Yusheng Zhao, Zhiyong Liu
Summary: Zhang et al. (2023) demonstrated through association analysis that the wheat E3 ubiquitin ligase TaAIRP2-1B positively regulates spike length by involving an upstream regulator, TaERF3, and a downstream target, TaHIPP3. This study provides a deeper understanding of spike architecture regulation and offers an alternative molecular module for designing wheat with increased yield potential.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Plant Sciences
Guoliang Han, Ziqi Qiao, Yuxia Li, Zongran Yang, Chengfeng Wang, Yuanyuan Zhang, Lili Liu, Baoshan Wang
Summary: RING zinc finger proteins play important roles in plant growth, development, and the response to abiotic stresses. They function as E3 ubiquitin ligases by modifying and degrading stress-related proteins in the cytoplasm and nucleus. Under abiotic stress, they may act as sensors or ABA receptors on the plasma membrane, or as transcription factors in the nucleus to regulate downstream gene expression.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
James Ross Terrell, Sijia Tang, Oluwafoyinsola Omobodunde Faniyi, In Ho Jeong, Jun Yin, Bhavitavya Nijampatnam, Sadanandan E. Velu, Wei Wang, Ruiwen Zhang, Ming Luo
Summary: Mouse double minute 2 homolog (MDM2) is an E3 ubiquitin-protein ligase that is involved in ubiquitin transfer and is elevated in cancer cells. This study solved crystal structures of MDM2 RING domain in complex with inhibitors Inulanolide A and MA242. The inhibitors bind at the C-terminus of MDM2 RING domain and disrupt interactions with the E2-Ubiquitin complex, inhibiting ubiquitin transfer for a broad spectrum of substrates.
Review
Biochemistry & Molecular Biology
Tianyuan Xie, Hai Qin, Zhengdong Yuan, Yiwen Zhang, Xiaoman Li, Lufeng Zheng
Summary: RING finger protein 168 (RNF168) is an E3 ubiquitin ligase that plays an important role in the DNA double-strand damage repair pathway. It has been found to be significantly involved in the occurrence and development of various cancers. Furthermore, RNF168 contributes to tumor cell drug resistance by enhancing DNA repair ability and regulating protein degradation. This paper summarizes and prospects the research progress of RNF168's structure, main functions, and its roles and mechanisms in tumorigenesis.
Article
Physiology
Gustavo R. Ares
Summary: This study found that cGMP mediates the ubiquitination of NKCC2 through the cullin-RING E3 ubiquitin ligase complex, thus regulating surface NKCC2 levels and reducing the reabsorption of sodium chloride. This finding provides new targets for the regulation of NKCC2 expression and activity.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Review
Virology
Chunfu Zheng, Yan-Dong Tang
Summary: MARCH proteins play critical roles in viral infections by directly antagonizing different steps of the viral life cycle and promoting viral infection.
Article
Plant Sciences
Jinhao Sun, Cuiling Yuan, Meng Wang, Anming Ding, Guohua Chai, Yuhe Sun, Gongke Zhou, Dahai Yang, Yingzhen Kong
Summary: In this study, the regulatory mechanisms of pectin demethylesterification were investigated, revealing that the mud1 Arabidopsis mutant has increased mucilage adherence due to increased PMEs activities and decreased DM. It was suggested that MUD1 may act as an ubiquitin ligase to regulate the DM of pectin by post-transcriptionally removing proteins that negatively regulate PMEs in seed coat mucilage.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
J. Wade Harper, Brenda A. Schulman
Summary: Cullin-RING ubiquitin ligases (CRLs) are modular platforms that regulate biological processes by ubiquitylating specific targets. Regulation is achieved through dynamic control of activation and substrate ubiquitylation, and CRLs provide a vast landscape for developing small molecules that reshape interactions and promote targeted turnover of proteins. Ultimately, the dynamics of substrate receptor interchange in CRLs drive the turnover of selected proteins to influence cellular decision-making.
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 90, 2021
(2021)
Review
Biochemistry & Molecular Biology
Alberto Coego, Jose Julian, Jorge Lozano-Juste, Gaston A. Pizzio, Abdulwahed F. Alrefaei, Pedro L. Rodriguez
Summary: Post-translational modifications, particularly protein ubiquitylation, play a crucial role in regulating protein function and stability in various aspects of plant biology. Ubiquitin code not only targets substrates for proteolytic degradation but also regulates protein interactions, activity, and localization through nonproteolytic functions. This review focuses on the ubiquitylation of ABA receptors and PP2C coreceptors, as well as other post-translational modifications of ABA receptors that lead to their ubiquitination and degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Huimin Sun, Jiatao Li, Xu Li, Qian Lv, Liuping Chen, Bingxin Wang, Liqun Li
Summary: TaSADR1 gene was found to negatively regulate drought stress response in wheat and achieved this function by regulating the expression of stress-related genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Review
Genetics & Heredity
Xiaoxue Chai, Qian Tao, Lili Li
Summary: This review highlights the pivotal role of RING finger proteins in chromatin remodeling, including their E3 ubiquitin ligase activities, modulation of ATP-dependent chromatin remodeling complexes, and interaction with histone post-translational modifications. The diverse biological effects of RING finger protein-mediated chromatin remodeling are discussed, along with potential therapeutic strategies for targeting these proteins.
Article
Agronomy
Daijing Zhang, Xiaoxu Zhang, Wu Xu, Tingting Hu, Jianhui Ma, Youfu Zhang, Jian Hou, Chenyang Hao, Xueyong Zhang, Tian Li
Summary: This study identified TaGW2L as a novel positive regulator of heading date in wheat. TaGW2L exhibited similar functions to TaGW2 and its overexpression led to an earlier heading date in wheat. This study sheds light on the role of TaGW2L in wheat heading date and provides potential applications for crop improvement.
Article
Biochemistry & Molecular Biology
Pian Liu, Wei Zhou, Liu Yang, Chen Zhang
Summary: The study reveals that RNF180 degrades RAD51 by ubiquitination, inhibiting the growth and metastasis of TNBC cells and sensitizing them to Gef.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Orsolya Bilkei-Gorzo, Tiaan Heunis, Jose Luis Marin-Rubio, Francesca Romana Cianfanelli, Benjamin Bernard Armando Raymond, Joseph Inns, Daniela Fabrikova, Julien Peltier, Fiona Oakley, Ralf Schmid, Anetta Hartlova, Matthias Trost
Summary: This study reveals the importance of phagosomal ubiquitylation and the E3 ubiquitin ligase RNF115 in regulating innate immune functions during bacterial infections.
Article
Biochemistry & Molecular Biology
Kaeli A. Welsh, Derek L. Bolhuis, Anneroos E. Nederstigt, Joshua Boyer, Brenda R. S. Temple, Thomas Bonacci, Li Gu, Alban Ordureau, J. Wade Harper, Joshua P. Steimel, Qi Zhang, Michael J. Emanuele, Joseph S. Harrison, Nicholas G. Brown
Summary: Polyubiquitination mediated by E2 and E3 enzymes is essential for cell cycle control, epigenetic regulation, and development. The helix-turn-helix (HTH) motif of the UBC has been shown to regulate the intrinsic polyubiquitination activity in different E2s, with distinct functions observed in UBE2S and UBE2R2. The repurposing of the E2(HTH) motif in these proteins suggests a new mechanism for modulating ubiquitin transfer and highlights the potential for additional regulation in E2-E3-mediated polyubiquitination.
Article
Cell Biology
Bo Dong, Shigeo Hayashi
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2015)
Article
Developmental Biology
Tetsuhisa Otani, Yosuke Ogura, Kazuyo Misaki, Takuya Maeda, Akiyo Kimpara, Shigenobu Yonemura, Shigeo Hayashi
Article
Developmental Biology
Guangxia Miao, Shigeo Hayashi
Article
Multidisciplinary Sciences
Kagayaki Kato, Bo Dong, Housei Wada, Miho Tanaka-Matakatsu, Yoshimasa Yagi, Shigeo Hayashi
NATURE COMMUNICATIONS
(2016)
Article
Cell Biology
Yosuke Ogura, Fu-Lai Wen, Mustafa M. Sami, Tatsuo Shibata, Shigeo Hayashi
DEVELOPMENTAL CELL
(2018)
Review
Genetics & Heredity
Shigeo Hayashi, Takefumi Kondo
Review
Genetics & Heredity
Shigeo Hayashi, Takefumi Kondo
Article
Multidisciplinary Sciences
Yuki Itakura, Sachi Inagaki, Housei Wada, Shigeo Hayashi
Article
Cell Biology
Yosuke Ogura, Mustafa M. Sami, Housei Wada, Shigeo Hayashi
Article
Biology
Takefumi Kondo, Shigeo Hayashi
Article
Cell Biology
Shigeo Hayashi, Yosuke Ogura
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2020)
Article
Cell Biology
Yuki Taira, Housei Wada, Shigeo Hayashi, Yuji Kageyama
Summary: In many animals, developmental stages are controlled by steroid hormones, with ecdysone playing a crucial role in Drosophila. Absence or interruption of embryonic ecdysone signaling results in defects in the tracheal system, with the gene pri being essential for cell fate decision in dorsal branches. These findings demonstrate the essential roles of ecdysone and its downstream target pri in tracheal development.
Article
Evolutionary Biology
David M. Linz, Yuichiro Hara, Kevin D. Deem, Shigehiro Kuraku, Shigeo Hayashi, Yoshinori Tomoyasu
Summary: This study used RNA sequencing to analyze the wing transcriptomes of two coleopteran species and identified genes associated with elytra and hindwings. RNA interference experiments revealed novel genes involved in the development of elytra morphology. These findings enhance our understanding of the evolution of beetle wings.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
(2023)
Article
Developmental Biology
Kentaro Yoshida, Shigeo Hayashi
Summary: Dying cells in the epithelia communicate with neighboring cells to initiate coordinated cell removal to maintain epithelial integrity. Epidermal growth factor (EGF) receptor (EGFR) signaling plays an important role in the maintenance of epithelial homeostasis. In Drosophila embryos, the enhanced extracellular signal-regulated kinase (ERK) signaling in epithelial tissues undergoing groove formation. EGFR mutant embryos show sporadic apical cell extrusion in the head, leading to a cascade of apical extrusions throughout the ventral body wall. This process is apoptosis dependent and can cause massive tissue disintegration in EGFR mutant epithelia. Tissue detachment from the vitelline membrane triggers the EGFR mutant phenotype. Therefore, EGFR not only promotes cell survival but also maintains epithelial integrity.
Review
Cell Biology
Shigeo Hayashi, Bo Dong
DEVELOPMENT GROWTH & DIFFERENTIATION
(2017)