期刊
GENES & DEVELOPMENT
卷 26, 期 1, 页码 92-104出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.179085.111
关键词
DNA restriction enzymes; DNA translocation; electron microscopy; small-angle scattering; molecular modeling; DNA translocases
资金
- Biotechnology and Biological Sciences Research Council [BB/D001870/1]
- Wellcome Trust [080304/Z/06/Z]
- Foundation for Polish Science [TEAM/2009-4/2]
- BBSRC [BB/D001870/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D522589/1] Funding Source: researchfish
Type I DNA restriction/modification (RM) enzymes are molecular machines found in the majority of bacterial species. Their early discovery paved the way for the development of genetic engineering. They control (restrict) the influx of foreign DNA via horizontal gene transfer into the bacterium while maintaining sequence-specific methylation (modification) of host DNA. The endonuclease reaction of these enzymes on unmethylated DNA is preceded by bidirectional translocation of thousands of base pairs of DNA toward the enzyme. We present the structures of two type I RM enzymes, EcoKI and EcoR124I, derived using electron microscopy (EM), small-angle scattering (neutron and X-ray), and detailed molecular modeling. DNA binding triggers a large contraction of the open form of the enzyme to a compact form. The path followed by DNA through the complexes is revealed by using a DNA mimic anti-restriction protein. The structures reveal an evolutionary link between type I RM enzymes and type II RM enzymes.
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