Article
Biochemistry & Molecular Biology
Youssef El Mouali, Milan Gerovac, Raminta Mineikaite, Joerg Vogel
Summary: FinO-domain proteins, as an emerging family of RNA-binding proteins, have diverse roles in bacterial post-transcriptional control and physiology, with the ability to act selectively or promiscuously on the same cellular RNA pool. The study extensively profiled cellular transcripts associated with the virulence plasmid-encoded FinO in Salmonella, identifying two major ligands: FinP and RepX. The research also observed cross-regulation of plasmids on the RNA level, with the FinO RBP controlling the replication of an antibiotic resistance plasmid in Salmonella.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Hyeong Jin Kim, Mazzen Black, Ross A. Edwards, Flora Peillard-Fiorente, Rashmi Panigrahi, David Klinger, Reiner Eidelpes, Ricarda Zeindl, Shiyun Peng, Jikun Su, Ayat R. Omar, Andrew M. MacMillan, Christoph Kreutz, Martin Tollinger, Xavier Charpentier, Laetitia Attaiech, J. N. Mark Glover
Summary: This study investigates the structural basis for RNA recognition by ProQ/FinO proteins and the importance of this recognition in repressing the uptake of environmental DNA in L. pneumophila. The authors also discover that other ProQ/FinO domains recognize transcriptional terminators with different specificities for the length of the 3' ssRNA tail.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Sara Correia Santos, Thorsten Bischler, Alexander J. Westermann, Joerg Vogel
Summary: The study reveals the important role of the Hfq-associated sRNA PinT in Salmonella virulence, showing its translational inhibition of the secreted effector kinase SteC and delay of actin rearrangements in infected host cells. The findings highlight the need for complementary methods to uncover the full target suites of sRNAs.
Article
Multidisciplinary Sciences
Jiandong Chen, Leann To, Francois de Mets, Xing Luo, Nadim Majdalani, Chin-Hsien Tai, Susan Gottesman
Summary: Small regulatory RNAs associated with the RNA chaperone Hfq play key roles in gene regulation in bacteria by altering mRNA translation and stability. Identification of new regulators that control iron homeostasis and other physiological traits suggest complex regulatory networks for sRNA signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Microbiology
Sabine Brantl, Peter Mueller
Summary: sRNAs act as abundant posttranscriptional regulators in all three kingdoms of life, with various methods being used to study chromosome-encoded sRNAs in bacteria over the past 20 years; while improved bioinformatics tools can predict potential sRNA targets, experimental confirmation remains challenging; known sRNAs in B. subtilis have defined targets and regulatory mechanisms.
Article
Biochemistry & Molecular Biology
Guillermo Gomez-Garcia, Angel Ruiz-Enamorado, Luis Yuste, Fernando Rojo, Renata Moreno
Summary: In the bacterium Pseudomonas putida KT2440, the transposase gene for ISPpu9 is regulated by two small RNAs, Asr9 and Ssr9, which play a role in enhancing tnp expression and promoting transposition. Asr9 stabilizes tnp mRNA and helps unfold its secondary structure, while Ssr9 may counteract Asr9 and keep tnp expression low.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ewelina M. Malecka, Daria Sobanska, Mikolaj Olejniczak
Summary: Bacterial small RNAs in association with the chaperone protein Hfq play a crucial role in regulating the expression of target mRNAs. This regulation involves sponge RNAs that sequester sRNAs affecting their regulatory output. Hfq facilitates the annealing of sponge RNAs to targeted sRNAs, suggesting a complex but efficient regulation mechanism.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Immunology
Derrick Watkins, Dev Arya
Summary: Hfq is essential for the interaction between sRNA and mRNA in many Gram-negative bacteria. However, in Gram-positive bacteria, sRNA can bind mRNA targets independently of Hfq. Although the global structure of Hfq is conserved, variations in the binding surfaces of Hfq result in the recognition of different RNA sequences.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Manuela Fuchs, Vanessa Lamm-Schmidt, Tina Lence, Johannes Sulzer, Arne Bublitz, Janet Wackenreuter, Milan Gerovac, Till Strowig, Franziska Faber
Summary: The anaerobic pathogen Clostridioides difficile forms antibiotic-resistant endospores to persist in the intestinal tract and cause recurrent infections. Through RIL-seq, we identified a network of small RNAs that bind to sporulation-related genes, and discovered that SpoX and SpoY regulate the translation of the master regulator of sporulation, Spo0A, in an opposing manner, leading to altered sporulation rates. Deletion mutants of SpoX and SpoY showed global effects on gut colonization and intestinal sporulation in antibiotic-treated mice. Our study uncovers a complex RNA-RNA interactome controlling the physiology and virulence of C. difficile and identifies a complex post-transcriptional layer in the regulation of spore formation in this important human pathogen.
Article
Plant Sciences
Daniel B. Nething, Abhijit Sukul, John W. Mishler-Elmore, Michael A. Held
Summary: The study highlights the potential regulatory role of sRNAs derived from CESA antisense transcripts in the control of cell wall biosynthesis, especially during the transition from primary to secondary cell wall biosynthesis. The data suggest that CESA antisense transcripts may function through an RNA-induced silencing mechanism, influencing the expression of cell wall gene networks.
Review
Microbiology
Kai Papenfort, Sahar Melamed
Summary: Small regulatory RNAs (sRNAs) play a key role in posttranscriptional gene control in bacteria, often modulating the expression of multiple genes. The regulatory properties of sRNAs and their interplay with transcription factors shape the output of regulatory networks. Additionally, sRNA activity can be antagonized by base-pairing with sponge RNAs, adding complexity to these networks.
ANNUAL REVIEW OF MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jorjethe Roca, Andrew Santiago-Frangos, Sarah A. Woodson
Summary: The pairwise interactions between bacterial sRNAs and Hfq determine whether the sRNAs are actively exchanged or stably coexist on the shared protein.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Tom Dendooven, Elisabeth Sonnleitner, Udo Blaesi, Ben F. Luisi
Summary: The bacterial RNA chaperone Hfq and its partner protein Crc form higher order assemblies on control regions of different P. aeruginosa target mRNAs. These assemblies have mRNA-specific quaternary architectures due to the combination of multivalent protein-protein interfaces and recognition of patterns in the RNA sequence. The structural polymorphism of these ribonucleoprotein assemblies enables selective translational repression of many different target mRNAs.
Article
Microbiology
Kyung Moon, Minji Sim, Chin-Hsien Tai, Kyungyoon Yoo, Charlotte Merzbacher, Sung-Huan Yu, David D. Kim, Jaehyun Lee, Konrad U. Forstner, Qing Chen, Scott Stibitz, Leslie G. Knipling, Deborah M. Hinton
Summary: Through transcriptomic analyses using RNA-seq and ANNOgesic, possible B. pertussis sRNAs were identified and their dependence on BvgAS was determined.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemistry & Molecular Biology
Alexandra Schilder, Boris Goerke
Summary: In enteric bacteria, small RNAs (sRNAs) such as MicC stimulate target RNA decay using RNase E. The interaction between the sRNA 5' monophosphate (5 ' P) and RNase E activates cleavage of the target. However, experimental evidence supporting this model is limited. To address this, researchers developed a genetic tool to generate controllable 5' monophosphorylated sRNAs. The results showed that while the 5' phosphorylation state determines stability of naturally triphosphorylated sRNAs, it does not play a major role in target RNA destabilization in vivo. Additionally, the C-terminal half of RNase E is crucial for MicC-mediated decay, suggesting the mandatory interaction with Hfq.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Microbiology
Pierre Mandin, Sylvia Chareyre, Frederic Barras
Review
Biochemistry & Molecular Biology
Beatrice Roche, Laurent Aussel, Benjamin Ezraty, Pierre Mandin, Beatrice Py, Frederic Barras
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2013)
Review
Biochemistry & Molecular Biology
Beatrice Roche, Laurent Aussel, Benjamin Ezraty, Pierre Mandin, Beatrice Py, Frederic Barras
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2013)
Article
Biochemistry & Molecular Biology
Edmund Loh, Olivier Dussurget, Jonas Gripenland, Karolis Vaitkevicius, Teresa Tiensuu, Pierre Mandin, Francis Repoila, Carmen Buchrieser, Pascale Cossart, Jorgen Johansson
Review
Microbiology
Pierre Mandin, Maude Guillier
CURRENT OPINION IN MICROBIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Pierre Mandin, Susan Gottesman
Article
Biochemistry & Molecular Biology
Pierre Mandin, Susan Gottesman
MOLECULAR MICROBIOLOGY
(2009)
Article
Biochemistry & Molecular Biology
Audrey Coornaert, Alisa Lu, Pierre Mandin, Mathias Springer, Susan Gottesman, Maude Guillier
MOLECULAR MICROBIOLOGY
(2010)
Article
Multidisciplinary Sciences
Toby Soper, Pierre Mandin, Nadim Majdalani, Susan Gottesman, Sarah A. Woodson
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2010)
Article
Genetics & Heredity
Sylvia Chareyre, Frederic Barras, Pierre Mandin
Review
Biochemistry & Molecular Biology
Pierre Mandin, Jorgen Johansson
MOLECULAR MICROBIOLOGY
(2020)
Article
Microbiology
Sylvia Chareyre, Pierre Mandin
MICROBIOLOGY SPECTRUM
(2018)
Article
Biochemistry & Molecular Biology
Pierre Mandin, Francis Repoila, Massimo Vergassola, Thomas Geissmann, Pascale Cossart
NUCLEIC ACIDS RESEARCH
(2007)
Article
Biochemistry & Molecular Biology
P Mandin, H Fsihi, O Dussurget, M Vergassola, E Milohanic, A Toledo-Arana, I Lasa, J Johansson, P Cossart
MOLECULAR MICROBIOLOGY
(2005)
