Article
Plant Sciences
Celia Municio, Wojciech Antosz, Klaus D. Grasser, Etienne Kornobis, Michiel Van Bel, Ignacio Eguinoa, Frederik Coppens, Andrea Braeutigam, Inna Lermontova, Astrid Bruckmann, Katarzyna Zelkowska, Andreas Houben, Veit Schubert
Summary: Condensins play a crucial role in shaping chromosomes and regulating gene expression. In plants, CAP-D3 influences chromosome organization and gene expression related to stress responses. The composition and function of condensin complexes in Arabidopsis are similar to those in other higher eukaryotes.
Article
Biology
Martin Houlard, Erin E. Cutts, Muhammad S. Shamim, Jonathan Godwin, David Weisz, Aviva Presser Aiden, Erez Lieberman Aiden, Lothar Schermelleh, Alessandro Vannini, Kim Nasmyth
Summary: The eukaryotic cell cycle is characterized by dramatic changes in chromosomal DNA morphology between interphase and mitosis. Cohesin and condensin enzymes extrude DNA loops to configure chromosomal DNA topology, with cohesin acting during interphase and condensin primarily during mitosis. MCPH1 gene mutations in patients with microcephaly and the deletion of Mcph1 in mouse embryonic stem cells unleash condensin II activity during interphase, leading to compact chromosomes in G1 and G2 phases. This process is inhibited by MCPH1's binding to condensin II's NCAPG2 subunit, highlighting the crucial role of MCPH1 in blocking condensin II's association with chromatin.
Article
Multidisciplinary Sciences
Abdelhalim Boukaba, Jian Liu, Carl Ward, Qiongfang Wu, Alexei Arnaoutov, Jierong Liang, Elena M. Pugacheva, Mary Dasso, Victor Lobanenkov, Miguel Esteban, Alexander V. Strunnikov
Summary: Many tumors express meiotic genes that can cause chromosome instability. The abnormal expression of meiotic cohesins is associated with chromosome missegregation, DNA damage, and altered gene expression. Additionally, meiotic cohesins localize to the same sites as BORIS/CTCFL, rather than the CTCF sites associated with somatic cohesins.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Didier Hodzic, Jun Wu, Karen Krchma, Andrea Jurisicova, Yonit Tsatskis, Yijie Liu, Peng Ji, Kyunghee Choi, Helen McNeill
Summary: This study reveals for the first time the crucial role of nuclear envelope protein NEMP1 in erythropoiesis. The researchers found that NEMP1 plays an important regulatory role in nuclear envelope openings and enucleation process of red blood cells. The results provide a valuable mouse model for studying defective erythropoiesis.
Article
Biochemistry & Molecular Biology
Maximilian F. D. Spicer, Daniel W. Gerlich
Summary: Chromosomes undergo transformations during the cell cycle, including transcription, replication, and segregation. These changes are driven by DNA loop extrusion and a chromatin solubility phase transition. Condensins enrich at an axial core, forming loops and providing resistance to spindle pulling forces. Mitotic chromosomes are further compacted by deacetylation of histone tails, making them insoluble and resistant to microtubule penetration. Ki-67 regulates surface properties, allowing independent chromosome movement in early mitosis and clustering during mitotic exit. Recent progress has shed light on the material properties of chromatin and how they facilitate accurate chromosome segregation.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2023)
Article
Biology
Sarah G. Swygert, Dejun Lin, Stephanie Portillo-Ledesma, Po-Yen Lin, Dakota R. Hunt, Cheng-Fu Kao, Tamar Schlick, William S. Noble, Toshio Tsukiyama
Summary: The study found that an increase in the range of local inter-nucleosomal contacts in quiescent yeast drives genome-wide compaction of chromatin fibers, leading to global repression of transcription. Unlike actively dividing cells, inter-nucleosomal interactions in quiescent cells require a specific basic patch in the histone H4 tail.
Article
Biology
Julian Haase, Richard Chen, Wesley M. Parker, Mary Kate Bonner, Lisa M. Jenkins, Alexander E. Kelly
Summary: The TFIIH complex plays a crucial role in maintaining chromosome compaction and condensin enrichment by dynamically altering the chromatin environment to facilitate condensin loading and condensin-dependent loop extrusion.
Review
Neurosciences
Du Pang, Shengping Yu, Xuejun Yang
Summary: Mitosis and meiosis are crucial life activities that transmit eukaryotic genetic information to progeny in a stable and orderly manner. The abnormal function of condensin, a large protein complex, has been found to cause chromatin concentration and genome organization disorder, leading to various diseases, especially neurological disorders. Understanding these mechanisms is important for understanding related diseases and discovering new clinical treatments.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Biology
Makoto M. Yoshida, Kazuhisa Kinoshita, Yuuki Aizawa, Shoji Tane, Daisuke Yamashita, Keishi Shintomi, Tatsuya Hirano
Summary: In vertebrates, condensin I and condensin II work together to assemble chromosomes during mitosis. However, our understanding of condensin II is limited. Through experiments, we discovered that one of the two HEAT subunits, CAP-D3, plays a crucial role in condensin II-mediated chromosome assembly, while the other HEAT subunit, CAP-G2, has a negative impact on this process. The ATPase protein and basic amino acid clusters of the kleisin subunit CAP-H2 are also essential. Deleting the C-terminal tail of CAP-D3 enhances the ability of condensin II to assemble chromosomes and reveals a hidden function of CAP-G2 in chromosome compaction.
Article
Obstetrics & Gynecology
Jordi Ribas-Maynou, Sergi Novo, Albert Salas-Huetos, Sergi Rovira, Marta Antich, Marc Yeste
Summary: This study found that defects in sperm chromatin protamination and condensation can have an impact on ICSI outcomes. Sperm protamination is related to fertilization rates and the in vitro capacity of sperm to condense their chromatin is linked to blastocyst rates, with these associations being more apparent in women.
HUMAN REPRODUCTION
(2023)
Article
Hematology
Zachary C. Murphy, Kristin Murphy, Jacquelyn Myers, Michael Getman, Tyler Couch, Vincent P. Schulz, Kimberly Lezon-Geyda, Cal Palumbo, Hongxia Yan, Narla Mohandas, Patrick G. Gallagher, Laurie A. Steiner
Summary: The terminal maturation of human erythroblasts involves significant changes in histone marks associated with active transcription elongation, rather than heterochromatin accumulation. Regulation of RNA polymerase II activity and gene expression at the transcriptional level play a critical role in controlling the maturation process of erythroblasts.
Article
Cell Biology
Md Golam Mostofa, Shamsul Morshed, Satoru Mase, Shun Hosoyamada, Takehiko Kobayashi, Takashi Ushimaru
Summary: The study reveals that Cdc14 protein phosphatase and topoisomerase II (Topo II) facilitate rDNA condensation after TORC1 inactivation, and are critical for cell survival and nucleolar protein degradation.
CELLULAR SIGNALLING
(2021)
Article
Multidisciplinary Sciences
Keishi Shintomi, Tatsuya Hirano
Summary: Topoisomerase II alpha plays a crucial role in mitotic chromatid assembly. By refining the mitotic chromatid reconstitution assay, researchers have gained new insights into the C-terminal domain (CTD) of topo II alpha. The CTD contributes to proper formation of nucleosome-depleted chromatids and fine-tunes the enzymatic core delivery process during mitotic chromatid assembly.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Parminder Kaur, Xiaotong Lu, Qi Xu, Elizabeth Marie Irvin, Colette Pappas, Hongshan Zhang, Ilya J. Finkelstein, Zhubing Shi, Yizhi Jane Tao, Hongtao Yu, Hong Wang
Summary: This study uses high-speed atomic force microscopy imaging to reveal that cohesin-NIPBL captures DNA through arm extension and assistance from feet, leading to the initiation of DNA loops independent of ATP hydrolysis. The feet are likely contributed by the C-terminal domains of SA1 and NIPBL and facilitate the loading of the cohesin complex onto DNA.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sumitabha Brahmachari, Vinicius G. Contessoto, Michele Di Pierro, Jose N. Onuchic
Summary: This article explores the link between genomic structure and function, highlighting the crucial role of physical manipulation of the genome by various proteins. By constructing a coarse-grained polymer model of the genome, the authors demonstrate how interactions among different classes of genomic interactions can drive predictable changes in global genome architecture and make testable predictions.
NUCLEIC ACIDS RESEARCH
(2022)
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Multidisciplinary Sciences
Jin Sun, Hui-Min Wei, Jiang Xu, Jian-Feng Chang, Zhihao Yang, Xingjie Ren, Wen-Wen Lv, Lu-Ping Liu, Li-Xia Pan, Xia Wang, Huan-Huan Qiao, Bing Zhu, Jun-Yuan Ji, Dong Yan, Ting Xie, Fang-Lin Sun, Jian-Quan Ni
NATURE COMMUNICATIONS
(2015)
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Multidisciplinary Sciences
Baoyu Zhao, Chang Shu, Xinsheng Gao, Banumathi Sankaran, Fenglei Du, Catherine L. Shelton, Andrew B. Herr, Jun-Yuan Ji, Pingwei Li
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Multidisciplinary Sciences
Tianyi Zhang, Fu-Ning Hsu, Xiao-Jun Xie, Xiao Li, Mengmeng Liu, Xinsheng Gao, Xun Pei, Yang Liao, Wei Du, Jun-Yuan Ji
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Multidisciplinary Sciences
Xin Guo, Chang Shu, Honggui Li, Ya Pei, Shih-Lung Woo, Juan Zheng, Mengyang Liu, Hang Xu, Rachel Botchlett, Ting Guo, Yuli Cai, Xinsheng Gao, Jing Zhou, Lu Chen, Qifu Li, Xiaoqiu Xiao, Linglin Xie, Ke K. Zhang, Jun-Yuan Ji, Yuqing Huo, Fanyin Meng, Gianfranco Alpini, Pingwei Li, Chaodong Wu
SCIENTIFIC REPORTS
(2017)
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Neurosciences
Tengfei Ma, Yifeng Cheng, Emily Roltsch Hellard, Xuehua Wang, Jiayi Lu, Xinsheng Gao, Cathy C. Y. Huang, Xiao-Yan Wei, Jun-Yuan Ji, Jun Wang
NATURE NEUROSCIENCE
(2018)
Article
Multidisciplinary Sciences
Yu Jia, Rong-Gang Xu, Xingjie Ren, Ben Ewen-Campen, Rajendhran Rajakumar, Jonathan Zirin, Donghui Yang-Zhou, Ruibao Zhu, Fang Wang, Decai Mao, Ping Peng, Huan-Huan Qiao, Xia Wang, Lu-Ping Liu, Bowen Xu, Jun-Yuan Ji, Qingfei Liu, Jin Sun, Norbert Perrimon, Jian-Quan Ni
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Genetics & Heredity
Yani Zheng, Yongbo Xue, Xingjie Ren, Mengmeng Liu, Xiao Li, Yu Jia, Ye Niu, Jian-Quan Ni, Yong Zhang, Jun-Yuan Ji
FRONTIERS IN GENETICS
(2018)
Article
Developmental Biology
Xinsheng Gao, Xiao-Jun Xie, Fu-Ning Hsu, Xiao Li, Mengmeng Liu, Rajitha-Udakara-Sampath Hemba-Waduge, Wu Xu, Jun-Yuan Ji
DEVELOPMENTAL BIOLOGY
(2018)
Editorial Material
Biochemistry & Molecular Biology
Jun-Yuan Ji, Chun Han, Wu-Min Deng
JOURNAL OF GENETICS AND GENOMICS
(2019)
Article
Multidisciplinary Sciences
Baoyu Zhao, Fenglei Du, Pengbiao Xu, Chang Shu, Banumathi Sankaran, Samantha L. Bell, Mengmeng Liu, Yuanjiu Lei, Xinsheng Gao, Xiaofeng Fu, Fanxiu Zhu, Yang Liu, Arthur Laganowsky, Xueyun Zheng, Jun-Yuan Ji, A. Phillip West, Robert O. Watson, Pingwei Li
Article
Genetics & Heredity
Xiao Li, Mengmeng Liu, Xingjie Ren, Nicolas Loncle, Qun Wang, Rajitha-Udakara-Sampath Hemba-Waduge, Stephen H. Yu, Muriel Boube, Henri-Marc G. Bourbon, Jian-Quan Ni, Jun-Yuan Ji
Article
Biochemistry & Molecular Biology
Wu Xu, Xiao-Jun Xie, Ali K. Faust, Mengmeng Liu, Xiao Li, Feng Chen, Ashlin A. Naquin, Avery C. Walton, Peter W. Kishbaugh, Jun-Yuan Ji
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Jin Sun, Xia Wang, Rong-Gang Xu, Decai Mao, Da Shen, Xin Wang, Yuhao Qiu, Yuting Han, Xinyi Lu, Yutong Li, Qinyun Che, Li Zheng, Ping Peng, Xuan Kang, Ruibao Zhu, Yu Jia, Yinyin Wang, Lu-Ping Liu, Zhijie Chang, Jun-Yuan Ji, Zhao Wang, Qingfei Liu, Shao Li, Fang-Lin Sun, Jian-Quan Ni
Summary: The study identifies HP1c as an essential epigenetic regulator of gut homeostasis in Drosophila, demonstrating its role in suppressing Notch signaling by interacting with key components of the pathway. The depletion of HP1c in Drosophila can be rescued by expressing human HP1 gamma, indicating its functional similarity to HP1c in Drosophila.
Article
Cell Biology
Qiong Wang, Ruofan Hu, Weihua Li, Yanhong Tai, Weiting Gu, Bhaskar C. Das, Fajun Yang, Junyuan Ji, Chenguang Wang, Jie Zhou
Summary: BF175 exhibited anti-tumor activity by targeting SREBP-dependent lipogenesis and inducing apoptosis through the mitochondrial pathway, suggesting its potential as a novel therapeutic agent for endometrial carcinoma.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Article
Cell Biology
Xiao Li, Meng Zhang, Mengmeng Liu, Tzu-Hao Liu, Rajitha-Udakara-Sampath Hemba-Waduge, Jun -Yuan Ji
Summary: This study found that phosphodeficient SREBP proteins are more stable and potent in promoting lipogenic gene expression and lipogenesis in fruit flies. Starvation blocks the effects of wild-type SREBP on lipogenic gene transcription, while phosphodeficient SREBP is resistant to this effect. The research also identified several conserved amino acid residues in the N-terminus region of SREBP that are essential for its interactions with Cdk8 and MED15.
DISEASE MODELS & MECHANISMS
(2022)