Article
Chemistry, Multidisciplinary
Laura Gentile, Antonio Casalini, Pietro Emmanuele, Riccardo Brusa, Annalisa Zaccaroni, Oliviero Mordenti
Summary: This study conducted a histological analysis on the gonads of European eels from North Adriatic lagoons and evaluated their maturation. The results showed that these eels have high levels of oocyte maturation and high GSI value indices at silvering stage III.
APPLIED SCIENCES-BASEL
(2022)
Article
Fisheries
Leander Hoehne, Jan-Dag Pohlmann, Marko Freese
Summary: In response to the declining European Eel stock, scientists explored a nonlethal method using photography to obtain essential biometrics and maturation stage from live eels, finding that it had the highest agreement with measurements from freshly killed eels. Additionally, they found it necessary to correct for shrinkage of eyes and pectoral fins after freezing to maximize reliability in stage classification.
MARINE AND COASTAL FISHERIES
(2023)
Article
Biotechnology & Applied Microbiology
Gabriel Schneebauer, Victoria Drechsel, Ron Dirks, Klaus Faserl, Bettina Sarg, Bernd Pelster
Summary: The study revealed the expression of a large number of transport proteins in rete capillaries of the European eel, enhancing the countercurrent concentrating ability of the rete. Contrary to expectations, the expression of transport proteins was not elevated in silver eels, and the rete tissue exhibited a high ROS defense capacity.
Article
Multidisciplinary Sciences
Martina Venditti, Catia Pedalino, Marion Rosello, Giulia Fasano, Malo Serafini, Celine Revenu, Filippo Del Bene, Marco Tartaglia, Antonella Lauri
Summary: Current genetic modification and phenotyping methods in teleost fish have greatly advanced genetic research and drug testing, with the small zebrafish becoming a popular alternative to large animals' experimentation. A minimally invasive method using tail fin scratching allows for early genotyping and reduces the number of animals used in mutant line generation.
SCIENTIFIC REPORTS
(2022)
Article
Surgery
Alessandro Giani, Tess van Ramshorst, Michele Mazzola, Claudio Bassi, Alessandro Esposito, Matteo de Pastena, Bjorn Edwin, Mushegh Sahakyan, Dyre Kleive, Asif Jah, Stijn van Laarhoven, Ugo Boggi, Emanuele Federico Kauffman, Riccardo Casadei, Claudio Ricci, Safi Dokmak, Fadhel Samir Fteriche, Steven A. White, Sivesh K. Kamarajah, Giovanni Butturini, Isabella Frigerio, Alessandro Zerbi, Giovanni Capretti, Elizabeth Pando, Robert P. Sutcliffe, Ravi Marudanayagam, Giuseppe Kito Fusai, Jean Michel Fabre, Bergthor Bjornsson, Lea Timmermann, Zahir Soonawalla, Fernando Burdio, Tobias Keck, Thilo Hackert, Bas Groot Koerkamp, Mathieu D'Hondt, Andrea Coratti, Patrick Pessaux, Andrea Pietrabissa, Bilal Al-Sarireh, Marco Marino, Quintus Molenaar, Vincent Yip, Marc Besselink, Giovanni Ferrari, Mohammad Abu Hilal
Summary: The study aimed to assess the best achievable outcomes in minimally invasive distal pancreatectomy with splenectomy using the Achievable Benchmark of Care method. The results showed that the benchmark values obtained using the ABC method represented optimal outcomes, including low complication rates and zero mortality. These benchmarks can be used to set standards and improve patient outcomes.
BRITISH JOURNAL OF SURGERY
(2022)
Article
Multidisciplinary Sciences
Elisa Benini, Kasun A. Bandara, Sebastian Nikitas Politis, Sofia Engrola, Anders Nielsen, Luis E. C. Conceicao, Andre Santos, Sune Riis Sorensen, Jonna Tomkiewicz
Summary: This study focuses on the nutritional requirements of European eel larvae during the larval first-feeding stage. Three experimental diets were tested, and diet 3 was found to be the most effective in promoting healthy development and survival of the larvae. Molecular analysis of digestion, appetite, and growth-related genes provided insights into the feeding status and growth of the larvae during the first-feeding stage.
Article
Fisheries
Pauline Jehannet, Arjan P. Palstra, Ignacio Gimenez Nebot, Henk Schipper, William Swinkels, Leon T. N. Heinsbroek, Hans Komen
Summary: The effects of eel-specific recombinant gonadotropins (rGTHs) on oocyte development in European eels were tested in this study. The results showed that treatment with recombinant luteinizing hormone (rLH) induced oocyte development in vitro, and treatment with recombinant follicle-stimulating hormone (rFSH) followed by rLH stimulated sexual maturation in vivo. Larvae were produced for the first time by rGTH treatment alone, but further optimization is needed for dosage and timing.
Article
Gastroenterology & Hepatology
Nicky van der Heijde, Frederique L. Vissers, Ugo Boggi, Safi Dokmak, Bjorn Edwin, Thilo Hackert, Igor E. Khatkov, Tobias Keck, Marc G. Besselink, Mohammed Abu Hilal
Summary: The European survey results showed considerable support for the European registry on MIPS, with many centers willing to participate and share their data. The study revealed that the most common MIPS procedure is laparoscopic distal pancreatectomy, while minimally invasive pancreatoduodenectomy is performed in over half of the centers surveyed.
Article
Surgery
Martin Wagner, Andreas Bihlmaier, Hannes Goetz Kenngott, Patrick Mietkowski, Paul Maria Scheikl, Sebastian Bodenstedt, Anja Schiepe-Tiska, Josephin Vetter, Felix Nickel, S. Speidel, H. Woern, F. Mathis-Ullrich, B. P. Mueller-Stich
Summary: The paper introduces a self-learning, context-sensitive, autonomous camera-guiding robot applicable to minimally invasive surgery and experimentally validates its effectiveness and performance improvement over time.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2021)
Article
Surgery
Benjamin Babic, Dolores T. Muller, Jin-On Jung, Lars M. Schiffmann, Paula Grisar, Thomas Schmidt, Seung-Hun Chon, Wolfgang Schroeder, Christiane J. Bruns, Hans F. Fuchs
Summary: This study compares the short-term outcomes of standardized RAMIE and HE and confirms the safety and feasibility of both procedures in a high-risk European population. RAMIE patients had a shorter ICU stay and a lower rate of postoperative complications. Both procedures showed excellent results in terms of lymph node harvest and R0 resection rates.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2022)
Article
Biochemical Research Methods
Yiqun Liu, Qi Yu, Le Ye, Li Yang, Yue Cui
Summary: For the first time, a wearable, minimally-invasive, fully electrochemically-controlled feedback minisystem for diabetes management is demonstrated. The minisystem integrates a biosensing device and an electrochemical micropump, both based on electrochemical reactions. The intelligent feedback system continuously monitors interstitial glucose levels and delivers insulin to control blood glucose within the normal range. The minisystem is characterized by its wearability, small size, cost-effectiveness, precision, stability, and painlessness.
Article
Fisheries
Ilaria Guarniero, Daniele Franchini, Alice Ferrari, Laura Gentile, Antonio Casalini, Pietro Emmanuele, Oliviero Mordenti
Summary: Due to its complex life cycle and multiple stress factors, the European eel has been declared Critically Endangered. Implementing mass production through artificial breeding protocols could be a possible solution to relieve pressure on natural stocks.
AQUACULTURE REPORTS
(2023)
Article
Anesthesiology
Nathan J. Vinzant, Jon M. Christensen, Suraj M. Yalamuri, Mark M. Smith, Gregory A. Nuttall, Arman Arghami, Allison M. LeMahieu, Darrell R. Schroeder, William J. Mauermann, Matthew J. Ritter
Summary: This study compared the analgesic efficacy of single-injection PECS II blocks and paravertebral blocks for elective robotic mitral valve surgery. The study found that patients receiving the PECS II block required fewer opioids in the immediate postoperative period and had comparable pain scores. No adverse outcomes were noted for either group.
JOURNAL OF CARDIOTHORACIC AND VASCULAR ANESTHESIA
(2023)
Review
Gastroenterology & Hepatology
Nehna Abdul Majeed, Asif A. Hitawala, Theo Heller, Christopher Koh
Summary: Hepatitis D viral infection in humans relies on hepatitis B for replication and transmission. Significant progress has been made in understanding the virus's life cycle, pathogenesis, diagnostics, and treatment. Diagnostic assays for hepatitis D have evolved, and monitoring its global impact is crucial. Non-invasive fibrosis markers are being explored for clinical monitoring. This review discusses virology, diagnostics, and non-invasive markers for fibrosis detection and monitoring in hepatitis D patients.
LIVER INTERNATIONAL
(2023)
Article
Surgery
Lianne Scholten, Sjors Klompmaker, Jony Van Hilst, Mario M. Annecchiarico, Gianpaolo Balzano, Riccardo Casadei, Jean-Michel Fabre, Massimo Falconi, Giovanni Ferrari, Mustafa Kerem, Igor E. Khatkov, Carlo Lombardo, Alberto Manzoni, Michele Mazzola, Niccolo Napoli, Edoardo E. Rosso, Pavel Tyutyunnik, Ulrich F. Wellner, David Fuks, Fernando Burdio, Tobias Keck, Mohammed Abu Hilal, Marc G. Besselink, Ugo Boggi
Summary: This multicenter retrospective study suggests that minimally invasive total pancreatectomy may be a valuable alternative to open total pancreatectomy, given the associated lower rate of major morbidity.
Article
Pharmacology & Pharmacy
Rob C. van Wijk, Wanbin Hu, Sharka M. Dijkema, Dirk-Jan van den Berg, Jeremy Liu, Rida Bahi, Fons J. Verbeek, Ulrika S. H. Simonsson, Herman P. Spaink, Piet H. van der Graaf, Elke H. J. Krekels
BRITISH JOURNAL OF PHARMACOLOGY
(2020)
Article
Multidisciplinary Sciences
Axel Meyer, Siegfried Schloissnig, Paolo Franchini, Kang Du, Joost M. Woltering, Iker Irisarri, Wai Yee Wong, Sergej Nowoshilow, Susanne Kneitz, Akane Kawaguchi, Andrej Fabrizius, Peiwen Xiong, Corentin Dechaud, Herman P. Spaink, Jean-Nicolas Volff, Oleg Simakov, Thorsten Burmester, Elly M. Tanaka, Manfred Schartl
Summary: Lungfishes, belonging to lobe-fined fish, possess a chromosome-quality genome with a vast size attributed to huge intergenic regions, introns with high repeat content, and active transposable elements. Phylogenomic analyses confirm lungfishes' key evolutionary position as the closest living relatives to tetrapods. Their preadaptations to living on land, increased rates of evolution, and duplication of genes associated with obligate air-breathing contribute to their tetrapod-like biology. These findings advance our understanding of the major transition during vertebrate evolution.
Article
Endocrinology & Metabolism
Junling He, Yi Ding, Natalia Nowik, Charel Jager, Muhamed N. H. Eeza, A. Alia, Hans J. Baelde, Herman P. Spaink
Summary: The study demonstrates that lepb regulates glucose homeostasis and adiposity in zebrafish, with lepb(-/-) mutant zebrafish showing features of T2DM. Additionally, lepb(-/-) adult zebrafish exhibit early signs of diabetic nephropathy, making them a promising model for researching the development and treatment of T2DM and its complications.
JOURNAL OF ENDOCRINOLOGY
(2021)
Article
Pathology
Rohola Hosseini, Gerda E. M. Lamers, Erik Bos, Pancras C. W. Hogendoorn, Abraham J. Koster, Annemarie H. Meijer, Herman P. Spaink, Marcel J. M. Schaaf
Summary: The study used zebrafish larvae infected with Mycobacterium marinum as an animal model for tuberculosis. The results showed that Myd88 deficiency led to more compact granuloma structures and a reduced number of leukocytes. Additionally, the majority of bacteria in the Myd88 mutant were located extracellularly, while most bacteria in the wild type were intracellular.
Article
Cell Biology
Wanbin Hu, Leonie van Steijn, Chen Li, Fons J. Verbeek, Lu Cao, Roeland M. H. Merks, Herman P. Spaink
Summary: In this study using zebrafish larval tail-wounding model, it was found that TLR2 and MyD88 play a role in regulating neutrophil and macrophage cell migration behavior, influencing the direction of neutrophil migration away from the wound and the speed of macrophage migration towards the wound edge. This indicates the involvement of TLR2 and MyD88 in responses to tail wounding by modulating leukocyte migration behavior and speed in vivo.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biology
Saloni Saxena, Herman P. Spaink, Gabriel Forn-Cuni
Summary: The review focuses on the rise in infections caused by nontuberculous mycobacteria (NTM) and their resistance to conventional antibiotics, proposing strategies to develop better therapies against them. It suggests accelerating the drug development pipeline by utilizing a combination of computational, laboratory and animal testing methods. The review also highlights the importance of understanding NTM virulence and developing new effective antimicrobial therapies.
Article
Toxicology
Bjorn E. Koch, Herman P. Spaink, Annemarie H. Meijer
Summary: Many facial bony features develop from preexisting collagen-rich cartilage structures through endochondral ossification. The proper development of these cartilage structures is crucial for facial morphological formation, which is sensitive to compounds that disturb histone acetylation patterns. A quantitative morphological assay using zebrafish larvae exposed to histone deacetylase inhibitors has been developed to measure the ceratohyal bone angle, providing a precise and sensitive proxy for overall facial cartilage development. This assay has been demonstrated to be applicable for investigating structure-activity relationships using valproic acid and related compounds.
TOXICOLOGY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Yi Ding, Marielle C. Haks, Gabriel Forn-Cuni, Junling He, Natalia Nowik, Amy C. Harms, Thomas Hankemeier, Muhamed N. H. Eeza, Jorg Matysik, A. Alia, Herman P. Spaink
Summary: This study investigates the effects of leptin deficiency in mice and zebrafish larvae through integration of metabolomics and transcriptomics, revealing similar metabolic alterations and dysregulated gene regulation related to proteolysis and arachidonic acid metabolism in both models.
CELL AND BIOSCIENCE
(2021)
Article
Cell Biology
Natalia Nowik, Tomasz K. Prajsnar, Anna Przyborowska, Krzysztof Rakus, Waldemar Sienkiewicz, Herman P. Spaink, Piotr Podlasz
Summary: Galanin, a conserved peptide found across different species, was traditionally thought to be related to neurotransmission but recent studies have shown its involvement in immune responses. Knockout of galanin in zebrafish increased susceptibility to Mycobacterium marinum and Staphylococcus aureus infections, while administration of a galanin analogue improved the fish's ability to control the infections. Transcriptional data revealed fewer gene regulations in response to mycobacterial infections in gal-/- mutants compared to gal+/+ counterparts, indicating a significant impact of galanin deficiency on immune-related pathways. The results suggest that galanin not only serves as a neurotransmitter, but also has implications in immune responses to bacterial infections, highlighting the complexity of the neuroendocrine system and its connection to immunity.
Article
Chemistry, Multidisciplinary
Doris A. van Bergeijk, Somayah S. Elsayed, Chao Du, Isabel Nunez Santiago, Anna M. Roseboom, Le Zhang, Victor J. Carrion, Herman P. Spaink, Gilles P. van Wezel
Summary: The biosynthetic gene clusters of Actinobacteria are poorly expressed in the laboratory. However, the stress hormone epinephrine has been found to promote their expression and influence the metabolism of Streptomyces. This response is mediated by the catechol moiety.
COMMUNICATIONS CHEMISTRY
(2022)
Article
Biology
Wanbin Hu, Herman P. Spaink
Summary: This review summarizes the functions of Toll-like receptor 2 (TLR2) in innate immunity and metabolism, and suggests future research directions.
Review
Medicine, Research & Experimental
Fleur M. Keij, Bjorn E. Koch, Fernando Lozano Vigario, Sinno H. P. Simons, Johan G. C. van Hasselt, H. Rob Taal, C. A. J. Knibbe, Herman P. Spaink, Irwin K. M. Reiss, Elke H. J. Krekels
Summary: Zebrafish larvae show promise as a preclinical model for neonatal sepsis due to their immune system similarities to humans. Research has demonstrated that zebrafish can be used to study infections causing neonatal sepsis, aiding in the exploration of new treatment approaches and biomarkers.
JOURNAL OF CLINICAL AND TRANSLATIONAL SCIENCE
(2021)
Article
Biochemical Research Methods
Niels M. Leijten, Petra Bakker, Herman P. Spaink, Jeroen den Hertog, Simone Lemeer
Summary: Thermal proteome profiling (TPP) enables unbiased detection of drug-target protein engagements in vivo. By using whole zebrafish embryo lysate, this study identified potential protein targets of napabucasin, including oxidoreductase PORA, which may affect Stat3 signaling.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Biology
M. Liem, T. Regensburg-Tuink, C. Henkel, H. Jansen, H. Spaink
Summary: Using only three ONT flow cells, thousands of organisms, including bacteriophages, were identified, with a large part at species level. Genomes could be assembled from environmental samples with Flye, resulting in >1 Mbp contigs in several cases and a near complete genome for a Thioglobus singularis species. k-mer analysis revealed that a significant portion of the data represented species not yet deposited in the database. This approach is suitable for scalable genomic investigations and provides a new platform for education in biodiversity.
BMC RESEARCH NOTES
(2021)
Article
Pharmacology & Pharmacy
Diyah Tri Utami, Sylvia Utami Tunjung Pratiwi, Herman P. Spaink, Tetiana Haniastuti, Triana Hertiani
Summary: This study investigated the efficacy of C-10 Massoia lactone in oral polymicrobial degradation, demonstrating its ability to degrade biofilms and destroy extracellular polymeric substances. Further research is needed to confirm these findings.
JOURNAL OF ADVANCED PHARMACEUTICAL TECHNOLOGY & RESEARCH
(2021)
Article
Endocrinology & Metabolism
Xiaomeng Li, Charles Brighton Ndandala, Qi Zhou, Chunyan Huang, Guangli Li, Huapu Chen
Summary: This study investigated the role of estrogen receptors (ERs) in vitellogenesis (Vtgs) regulation in pompano fish. The findings suggest that E2 may regulate the expression of different subtypes of vtg through ERs, displaying a compensatory expression effect on the regulation of ers and vtgs. This provides a theoretical basis for further research on reproductive endocrinology in pompano fish.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2024)
Article
Endocrinology & Metabolism
Norio Kansaku, Takeshi Ohkubo
Summary: Endocrine changes during bird reproduction, particularly the relationship between prolactin and incubation behavior, were investigated. The study monitored the physiological status and incubation behavior of Silkie hens over 1-2 years. The results showed that most mature hens exhibited incubation behavior multiple times, and there was a noticeable increase in nest occupancy 7-10 days before incubation onset. Protein analysis revealed that secreted prolactin during the reproductive cycle contains various isoforms, suggesting post-translational modifications like glycosylation and phosphorylation.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2024)
Article
Endocrinology & Metabolism
Minghui Li, Lina Sun, Linyan Zhou, Deshou Wang
Summary: This article summarizes the importance of the Nile tilapia as an excellent animal model for studying reproductive endocrinology in fish, with a focus on the crucial role of estrogen in female development.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2024)
Article
Endocrinology & Metabolism
Breanna N. Harris, Carolyn M. Bauer, James A. Carr, Caitlin R. Gabor, Jennifer L. Grindstaff, Caleigh Guoynes, Jennifer J. Heppner, Cris C. Ledon-Rettig, Patricia C. Lopes, Sharon E. Lynn, Carla B. Madelaire, Lorin A. Neuman-Lee, Maria G. Palacios, Paul Soto, Jennifer Terry
Summary: The COVID-19 pandemic had a significant impact on academics, particularly on those with minoritized identities or who were early career, caregivers, or had intersecting identities. The pandemic altered the impact factors of academia, including scholarly products, and affected the way individuals could respond. It is predicted that the pandemic will have long-term impacts on the population dynamics, composition, and landscape of the academic ecosystem. The number of journal submissions decreased, especially among women authors, and the pandemic heavily impacted women authors from Asia and the Middle East.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2024)
