期刊
GENERAL AND COMPARATIVE ENDOCRINOLOGY
卷 186, 期 -, 页码 150-156出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2013.02.037
关键词
Goldfish ghrelin; Salmon GnRH; Primary goldfish pituitary cell culture; Maturational gonadotropin and growth hormone release; Differential actions of GHRH
资金
- NSERC [121399]
- Department of Biological Sciences, University of Alberta
- Queen Elizabeth II postgraduate scholarship
- NSERC PGSD
Goldfish GH-releasing hormone (gGHRH) has been recently identified and shown to stimulate GH release in goldfish. In goldfish, neuroendocrine regulation of GH release is multifactorial and known stimulators include goldfish ghrelin (gGRLN(19)) and salmon gonadotropin-releasing hormone (sGnRH), factors that also enhance LH secretion. To further understand the complex regulation of pituitary hormone release in goldfish, we examined the interactions between gGHRH, gGRLN(19), and sGnRH on GH and LH release from primary cultures of goldfish pituitary cells in perifusion. Treatment with 100 nM gGHRH for 55 mm stimulated GH release. A 5-min pulse of either 1 nM gGRLN(19) or 100 nM sGnRH induced GH release in naive cells, and these were just as effective in cells receiving gGHRH. Interestingly, gGHRH abolished both gGRLN(19)- and sGnRH-induced LH release and reduced basal LH secretion levels. These results suggest that gGHRH does not interfere with sGnRH or gGRLN(19) actions in the goldfish somatotropes and further reveal, for the first time, that GHRH may act as an inhibitor of stimulated and basal LH release by actions at the level of pituitary cells. (C) 2013 Elsevier Inc. All rights reserved.
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