Egg size and yolk steroids vary across the laying order in cockatiel clutches: A strategy for reinforcing brood hierarchies?

When a female bird begins incubation before clutch completion, the nestlings hatch sequentially, and a size hierarchy forms within the brood. This size hierarchy may be minimized or exacerbated through differential allocation of resources to eggs across the laying order. In this study, we characterize intra-clutch variation in cockatiel clutches by measuring egg mass, yolk mass, and concentrations of yolk testosterone, androstenedione, and corticosterone. Cockatiels are a long-lived member of the Psittaciformes. Because asynchronous hatching may reduce sibling competition and allow for extended development periods in long-lived birds, we predicted that female cockatiels would allocate maternal resources in a way that would reinforce the brood size hierarchy. Significant within-clutch differences in egg size and steroid concentrations were observed. Eggs at the end of the laying sequence were smaller and had significantly smaller yolks than eggs early in the laying order. Fifth-laid eggs, as well as first-laid eggs, contained significantly lower concentrations of testosterone than eggs in other positions of the laying sequence. No differences in yolk androstenedione concentration were observed. Yolk corticosterone concentrations increased linearly with laying order. Together, these patterns might reinforce the brood size hierarchy created by asynchronous hatching. (C) 2010 Elsevier Inc. All rights reserved.