期刊
GENERAL AND COMPARATIVE ENDOCRINOLOGY
卷 160, 期 1, 页码 19-29出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2008.10.011
关键词
Estrogen receptor; Eatradiol; Calcium; Immunolocalization; Osteoclast; Teleost fish; Elasmoid scale
资金
- European Social Fund
- National Funds [POCTI/CVC/39355/2001]
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/ 5198/2001]
- PRODEP, Programa de Desenvolvimento Educativo para Portugal
Calcium mobilization from internal stores, such as scales, induced by 17 beta-estradiol during sexual maturation in salmonids is well documented. This calcium mobilization from scales is proposed to be mediated by the estrogen receptor (ER). However, the ER subtypes involved and signaling mechanisms responsible for this effect remain to be fully characterized. In the present study, we have localized ER alpha, ER beta a and ER beta b proteins in juvenile and adult sea bream (Spares auratus) and Mozambique tilapia (Oreochromis mossambicus) scales by immunohistochemistry with sea bream ER subtype specific antibodies. The three ERs were detected in isolated or small groups of round cells, in the basal layer of the scales of both juvenile and adult fish and the localization and signal intensity varied with the species and age of the animals. The ERs may be co-localized in cells of the scale posterior region that expressed tartrate-resistant acid phosphatase (TRAP), a marker for osteoclasts. These results suggest that the calcium mobilizing action of 17 beta-estradiol on fish scales is via its direct action on ERs localized in osteoclasts. (C) 2008 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据