期刊
GENE THERAPY
卷 18, 期 5, 页码 509-516出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/gt.2010.174
关键词
T-cell; NK cell; chimeric receptor; cancer immunotherapy; myeloma
类别
资金
- Norris Cotton Cancer Center at Dartmouth Medical School, Department of Microbiology and Immunology
- National Institutes of Health [CA130911, T32 AI07363, P20 RR16437]
Multiple myeloma causes approximately 10% of all hematologic malignancies. We have previously shown that human T cells expressing chimeric NKG2D receptors (chNKG2D) consisting of NKG2D fused to the CD3 zeta cytoplasmic domain secrete proinflammatory cytokines and kill human myeloma cells. In this study, we show chNKG2D T cells are effective in a murine model of multiple myeloma. Mice with established 5T33MM-green fluorescent protein tumors were treated with one or two infusions of chNKG2D T cells. Compared with mice treated with T cells expressing wild type (wt)NKG2D receptors, a single dose of chNKG2D T cells increased survival, with half of the chNKG2D T-cell-treated mice surviving long term. Two infusions of chNKG2D T cells led to tumor-free survival in all mice. ChNKG2D T cells were locates at sites of tumor growth, including the bone marrow and spleen after intravenous injection. There was an increase in activated host T cells and NK cells at tumor sites and in serum interferon-gamma after chNKG2D T-cell injection. Surviving mice were able to resist a rechallenge with 5T33MM cells but not RMA lymphoma cells, indicating that the mice developed a protective, specific memory response. These data demonstrate that chNKG2D T cells may be an effective adoptive cellular therapy for multiple myeloma. Gene Therapy (2011) 18, 509-516; doi:10.1038/gt.2010.174; published online 6 January 2011
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