Article
Oncology
Willi Jugel, Stefanie Tietze, Jennifer Daeg, Dietmar Appelhans, Felix Broghammer, Achim Aigner, Michael Karimov, Gabriele Schackert, Achim Temme
Summary: A new hybrid polyplex has been developed for targeted gene therapy, efficiently delivering genes to specific tumor cells. This approach may have significant implications for cancer treatment.
Article
Pharmacology & Pharmacy
Hardy Mitdank, Simko Sama, Meike Troeger, Maria Francesca Testa, Mattia Ferrarese, Dario Balestra, Mirko Pinotti, Alexander Weng
Summary: Minicircle DNA is a promising tool in gene therapy with advantages such as higher transfection efficiency and longer expression time, but its cost-intensive production limits broader use. An optimized production method can lead to high-quality minicircle DNA, which has been investigated for pharmaceutical potential in vitro in a tumor cell model.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Immunology
Qi Zhang, Jing Pan, Donghai Xiong, Junjun Zheng, Kristi N. McPherson, Sangbeom Lee, Mofei Huang, Yitian Xu, Shu-hsia Chen, Yian Wang, Lea Hildebrandt Ruiz, Ming You
Summary: Delivery of aerosolized microRNAs targeting PD-L1 can effectively prevent the development and progression of lung cancer. In this study, aerosolized miR-138-5p and miR-200c miRNA mimics were found to inhibit B(a)P-induced lung adenomas and NTCU-induced lung squamous cell carcinomas with no detectable side effects. These miRNAs were also shown to inhibit PD-L1 expression and promote the immune response against cancer cells by increasing CD4+ and CD8+ T cells while reducing PD-1 expression and T-regulatory cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Torben Sebastian Last, Simone Pagliano, Theocharis Nikiforos Iordanidis, Frank Niklaus, Goran Stemme, Niclas Roxhed
Summary: The functional area of silicon-based MEMS devices is usually small compared to the overall chip size. The desire to minimize chip size is driven by cost, but the challenges of handling such small devices cannot be solved by standard packaging methods. This study demonstrates the manufacturing and packaging of the world's smallest spray nozzle chip for drug delivery to the lung, and shows how magnetic assembly and microfluidic glue fixation can overcome the barriers associated with small MEMS devices.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Pharmacology & Pharmacy
Pratik Kulkarni, Deepak Rawtani, Sejal Rajpurohit, Shyam Vasvani, Tejas Barot
Summary: Hyaluronic acid was loaded into niosomes and showed stable release and localization in the lungs through the aerosol route.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Aravind George, Farzan Akbaridoust, Nurul A. Zainal Abidin, Warwick S. Nesbitt, Ivan Marusic
Summary: Hydrodynamic trapping of particles in micro-nano scale applications has provided significant insights. Among non-contact methods, image-based real-time control in cross-slot microfluidic devices shows promise for single cellular assays. Experiments in different channels with varying control delay and magnification achieved sustained trapping of 5 μm diameter particles at high strain rates. The maximum attainable strain rate depends on the control delay and particle resolution, and with further improvements, higher strain rates can be achieved for single cellular assay studies.
Article
Chemistry, Multidisciplinary
Dongxiao Yin, Mengjie Zhang, Jiaxin Chen, Yuanyu Huang, Dehai Liang
Summary: This study designed a series of cationic peptides with the same charge density but varying hydrophobicity and capacity to form hydrogen bonds, and investigated their ability to form complexes with siRNA and their behavior in the presence of serum. The hydrophobic interaction controls the complexation between peptide and siRNA, while hydrogen bonds are responsible for binding peptides to serum components.
CHINESE CHEMICAL LETTERS
(2021)
Article
Immunology
Zhannan Wang, Xiaohan Zhao, Ying Wang, Chao Sun, Ming Sun, Xingyun Gao, Futing Jia, Chenxin Shan, Guilian Yang, Jianzhong Wang, Haibin Huang, Chunwei Shi, Wentao Yang, Aidong Qian, Chunfeng Wang, Yanlong Jiang
Summary: The use of a dual promoter expression plasmid and self-lysed Salmonella strain as a delivery vesicle in the pYL87 construction showed promising results in vaccine design, enhancing immune responses and providing increased protection against genotype VII NDV compared to the previous pYL47 vector.
Article
Immunology
Francisco Callejas-Hernandez, Alfonso Herreros-Cabello, Javier del Moral-Salmoral, Manuel Fresno, Nuria Girones
Summary: This study analyzed the complete repertoire of maxicircle and minicircle sequences of different T. cruzi strains, revealing structural differences in minicircles and maxicircles among strains. Understanding the mitochondrial genome of Trypanosomatids is crucial for coordinating the expression of nuclear and mitochondrial genes involved in oxidative phosphorylation, and the heterogeneity observed in minicircles and maxicircles suggests a more complex organization than previously described.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Austin H. Williams, Adrien M. Hebert, Robert C. Boehm, Mary E. Huddleston, Meghan R. Jenkins, Orlin D. Velev, M. Tyler Nelson
Summary: Highly porous and ultrasoft polymeric mats mimicking human tissues were formed from novel polyurethane soft dendritic colloids, which have a unique fibrillar morphology. The mechanical properties of the mats can be tuned for physiological relevance, and they were found to support cellular growth and viability, with implications for nanoparticle uptake and cytokine secretion.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Olympia Bikou, Serena Tharakan, Kelly P. Yamada, Taro Kariya, Jaume Aguero, Alexandra Gordon, Renata Mazurek, Tadao Aikawa, Erik Kohlbrenner, Kenneth M. Fish, Roger J. Hajjar, Kiyotake Ishikawa
Summary: A disappointing number of new therapies for pulmonary hypertension (PH) have been successfully translated to the clinic. This study demonstrates that endobronchial aerosolization delivery is an effective method for in-lung gene therapy in PH and holds promise for clinical application.
HUMAN GENE THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Shanru Zuo, Xueguang Li, Yide Yang, Junhua Zhou, Quanyuan He
Summary: EccDNA, a special type of circular DNA, is suggested to have important biological functions in regulating downstream processes. However, the specific functions of individual eccDNAs remain unclear due to limited methods for synthesizing eccDNAs. This study introduces a novel method called QuickLAMA, which enables rapid synthesis of eccDNAs up to 2.6 kb using a simple PCR and ligation approach. The method is efficient, cost-effective, time-efficient, and easily standardized, with the potential to produce a wide range of desired eccDNAs and promote clinical applications of eccDNA research.
Article
Multidisciplinary Sciences
Olivier Reynard, Claudia Gonzalez, Claire Dumont, Mathieu Iampietro, Marion Ferren, Sandrine Le Guellec, Lajoie Laurie, Cyrille Mathieu, Gabrielle Carpentier, Georges Roseau, Francesca T. Bovier, Yun Zhu, Deborah Le Pennec, Jerome Montharu, Amin Addetia, Alexander L. Greninger, Christopher A. Alabi, Elise Brisebard, Anne Moscona, Laurent Vecellio, Matteo Porotto, Branka Horvat
Summary: Researchers demonstrated the efficacy of an aerosolized lipopeptide fusion inhibitor against the measles virus using a cynomolgus macaque model and mesh nebuliser administration. They used a custom-designed nebulizer to ensure effective delivery of the peptide to the respiratory tract without adverse effects in macaques. This study provides a proof-of-concept for aerosol delivery of fusion inhibitory peptides as a means to prevent respiratory infections, including measles, in non-vaccinated individuals.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
Xueshuai Ye, Yongqiang Wu, c Haiqiang Zhang, de Long Shi, Ye Zhou, Sumin Guo, Jiantao Dong, Jianhui Cai
Summary: Manufacturing CAR-T cells using viral vectors is expensive and time-consuming, and random integration of genes encoding CARs can lead to oncogenesis or the production of devastating CAR-tumor cells. In this study, we developed a virus-free and non-transgenic mcDNA vector to rapidly generate CD19 CAR-T cells within two days. Furthermore, we demonstrated that the antitumor effects of CD19 CAR-T cells produced by mcDNA are as effective as those produced by viral vectors in vitro and in xenograft models. Our manufacturing process also avoids the production of fatal CAR-tumor cells.
Article
Microbiology
Zhannan Wang, Ying Wang, Chao Sun, Xiaohan Zhao, Ming Sun, Xingyun Gao, Futing Jia, Tongyu Zhang, Chongbo Ge, Xiao Zhang, Menglei Zhang, Guilian Yang, Jianzhong Wang, Haibin Huang, Chunwei Shi, Wentao Yang, Xin Cao, Nan Wang, Yan Zeng, Chunfeng Wang, Yanlong Jiang
Summary: This study demonstrated that the application of mcDNA technology dramatically increased the DNA vaccine efficiency, leading to a stronger immune response in chickens and providing additional support for the use of our mcDNA platform in the veterinary field.
VETERINARY MICROBIOLOGY
(2022)
Article
Infectious Diseases
Andrew Chou, Marylette Roa, Michael A. Evangelista, Arielle Kae Sulit, Evelina Lagamayo, Brian C. Torres, David C. Klinzing, Maria Luisa G. Daroy, Josephine Navoa-Ng, Richard Sucgang, Lynn Zechiedrich
MICROBIAL DRUG RESISTANCE
(2016)
Editorial Material
Biology
David Bates, B. Montgomery Pettitt, Gregory R. Buck, Lynn Zechiedrich
PHYSICS OF LIFE REVIEWS
(2016)
Article
Biochemistry & Molecular Biology
Qian Wang, Rossitza N. Irobalieva, Wah Chiu, Michael F. Schmid, Jonathan M. Fogg, Lynn Zechiedrich, B. Montgomery Pettitt
NUCLEIC ACIDS RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
Anna H. Bizard, Xi Yang, Helene Debat, Jonathan M. Fogg, Lynn Zechiedrich, Terence R. Strick, Florence Garnier, Marc Nadal
NUCLEIC ACIDS RESEARCH
(2018)
Article
Multidisciplinary Sciences
Truston J. Bodine, Michael A. Evangelista, Huan Ting Chang, Christopher A. Ayoub, Buck S. Samuel, Richard Sucgang, Lynn Zechiedrich
Review
Genetics & Heredity
Cinnamon L. Hardee, Lirio Milenka Arevalo-Soliz, Benjamin D. Hornstein, Lynn Zechiedrich
Article
Microbiology
Zachary C. Conley, Truston J. Bodine, Andrew Chou, Lynn Zechiedrich
Article
Multidisciplinary Sciences
Zachary C. Conley, Kimberly M. Carlson-Banning, Ashley G. Carter, Alejandro de la Cova, Yongcheng Song, Lynn Zechiedrich
Article
Immunology
Brittani N. Blunck, Letisha Aideyan, Xunyan Ye, Vasanthi Avadhanula, Laura Ferlic-Stark, Lynn Zechiedrich, Brian E. Gilbert, Pedro A. Piedra
Summary: This study evaluated the serum antibody response to the respiratory syncytial virus (RSV) protein p27 following natural RSV reinfection in adults. The results showed specific patterns of antibody response depending on the infection status and different RSV subtypes.
Article
Multidisciplinary Sciences
Jonathan M. Fogg, Allison K. Judge, Erik Stricker, Hilda L. Chan, Lynn Zechiedrich
Summary: The study unveils the dynamic relationship between DNA supercoiling-induced sequence-dependent disruptions to base pairing, DNA looping, and the shape of the DNA molecule. Negative supercoiling of DNA can lead to disruptions in base pairing at superhelical apices, allowing for DNA writhe to relieve torsional strain.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Brittani N. Blunck, Laura S. Angelo, David Henke, Vasanthi Avadhanula, Matthew Cusick, Laura Ferlic-Stark, Lynn Zechiedrich, Brian E. Gilbert, Pedro A. Piedra
Summary: This study investigated the longevity of memory T cell responses to the RSV fusion protein following natural RSV infection in adults. The results showed that memory T cell responses mirrored antibody responses and exhibited subtype-specific differences. Some individuals demonstrated a deficiency in the generation of a durable RSV-specific adaptive immune response, indicating a higher risk of re-infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Proceedings Paper
Biochemistry & Molecular Biology
Jonathan M. Fogg, Lynn Zechiedrich
TOPOLOGY AND GEOMETRY OF BIOPOLYMERS
(2020)
Article
Multidisciplinary Sciences
Lynn Zechiedrich, Jonathan M. Fogg
TECHNOLOGY AND INNOVATION
(2019)
