期刊
GENE
卷 545, 期 1, 页码 45-55出版社
ELSEVIER
DOI: 10.1016/j.gene.2014.05.008
关键词
Arabidopsis thaliana; Brassica napus; FAD2; Intron; Microsomal oleate acid desaturase
资金
- National Natural Science Foundation of China [31000722, 31201240]
- National High Technology Research and Development Program of China [2012AA101107-3, 2011AA10A104]
- Major State Basic Research Development Program of China [2006-CB101600]
- Program for Innovative Research Team in University of the Chinese Ministry of Education [IRT1239]
In the present study, we characterized the transcriptional regulatory region (KF038144) controlling the expression of a constitutive FAD2 in Brassica napus. There are multiple FAD2 gene copies in B. napus genome. The FAD2 gene characterized and analyzed in the study is located on chromosome A5 and was designated as BnFAD2A5-1. BnFAD2A5-1 harbors an intron (1192 bp) within its 5'-untranslated region (5'-UTR). This intron demonstrated promoter activity. Deletion analysis of the BnFAD2A5-1 promoter and intron through the beta-glucuronidase (GUS) reporter system revealed that the -220 to -1 bp is the minimum promoter region, while - 220 to -110 bp and +34 to +285 bp are two important regions conferring high-levels of transcription. BnFAD2 transcripts were induced by light, low temperature, and abscisic acid (ABA). These observations demonstrated that not only the promoter but also the intron are involved in controlling the expression of the BnFAD2A5-1 gene. The intron-mediated regulation is an essential aspect of the gene expression regulation. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据