期刊
GENE
卷 552, 期 1, 页码 59-66出版社
ELSEVIER
DOI: 10.1016/j.gene.2014.09.013
关键词
Chrysanthemum nankingense; Heat stress; RNA-Seq; Stress response
资金
- Program for New Century Excellent Talents in University of Chinese Ministry of Education [NCET-12-0890, NCET-11-0669, NCET-10-0492]
- National Natural Science Foundation of China [31171987, 31272196, 31372100]
- Natural Science Fund of Jiangsu Province [BK201164, BK2012773]
- Fundamental Research Funds for the Central Universities [KYZ201147]
- College Graduate Research and Innovation Plan of Jiangsu Province [CXZZ12_0286]
The RNA-Seq platform was used to characterize the high-temperature stress response of Chrysanthemum nankingense. A set of 54,668 differentially expressed unigenes was identified. After a threshold of ratio change >= 2 and a q-value of <0.05 were applied, the number of differentially transcribed genes was reduced to 3955, of which 765 were up-regulated and 3190 were down-regulated in response to heat stress. The differentially transcribed genes were predicted to participate in 26 biological processes, 4 cellular components, and 13 molecular functions. Among the most differentially expressed genes between the two libraries were well-recognized high-temperature responsive protein families, such as heat shock factors and heat shock proteins, various transcription factor families, and a number of RNA metabolism-related genes. Overall, the RNA-Seq analyses revealed a high degree of transcriptional complexity in early heat stress response. Some of these high-temperature responsive C. nankingense genes may prove useful in efforts to improve thermotolerance of commercial chrysanthemum. (C) 2014 Elsevier B.V. All rights reserved.
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