Article
Cell Biology
Shiho Torii, Chikako Ono, Rigel Suzuki, Yuhei Morioka, Itsuki Anzai, Yuzy Fauzyah, Yusuke Maeda, Wataru Kamitani, Takasuke Fukuhara, Yoshiharu Matsuura
Summary: A PCR-based, bacterium-free method has been developed to generate SARS-CoV-2 infectious clones, allowing for a better understanding of the virus through the construction of reporter and mutant viruses.
Article
Multidisciplinary Sciences
Akina Nakamura, Tomohiro Kotaki, Yurie Nagai, Shunta Takazawa, Kenzo Tokunaga, Masanori Kameoka
Summary: Replicon RNA (repRNA) vaccine is a promising vaccine platform that can self-replicate and act as an adjuvant, generating robust immunity. This study successfully constructed and evaluated a repRNA vaccine that induced high levels of T-cell response but had minimal impact on antibody response. Future work should focus on optimizing vaccine administration methods and improving S protein expression to enhance the immune-inducing ability of the repRNA vaccine.
Article
Immunology
Hae-Gwang Jung, Hee Cho, Minwoo Kim, Haewon Jung, Yeonju Bak, Se-Young Lee, Han Young Seo, Yu-Min Son, Hawon Woo, Gone Yoon, Seong-Jun Kim, Jong-Won Oh
Summary: This study demonstrates the significant impact of nonstructural proteins of the Zika virus on viral replication and pathogenicity, particularly in immunocompromised hosts.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Gang Luo, Li Wang, Shenqiang Hu, Kun Du, Jie Wang, Songjia Lai
Summary: This study found that the expression levels of the leptin gene in different tissues of Tianfu black rabbits are correlated with meat quality traits. The leptin gene may be one of the important candidate genes for meat quality traits.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Virology
Ying Huang, Hongshan Xu, Shan Liu, Jiansheng Lu, Lili Jia, Yuhua Li, Rong Wang, Peng Yang, Yongxin Yu, Zhixin Yang
Summary: The intron-based stabilization approach is useful for stable flavivirus infectious clone construction. A highly attenuated Japanese encephalitis live vaccine strain (SA(14)-14-2) was used as a backbone vector to develop recombinant vaccines. However, a rescued virus strain (rHV-DJEV) with increased neurovirulence was accidentally acquired during the experiments, indicating the need for caution in using the intron-based stabilization approach in vaccine development.
ARCHIVES OF VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Federico Colombo, Elisa Corsiero, Myles J. Lewis, Costantino Pitzalis
Summary: This study aims to strengthen the observation that there is no evidence of genetic manipulation in SARS-CoV-2 by detecting classical restriction site sequences in human genomes and comparing them with experimental recombinant SARS-CoV-like viruses. A novel approach is proposed to generate a restriction endonucleases site map of SARS-CoV-2 and related coronaviruses for tracking virus evolution.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Xinrong Zhou, Lei Zhou, Pingping Zhang, Xinna Ge, Xin Guo, Jun Han, Yongning Zhang, Hanchun Yang
Summary: In this study, Chinese researchers isolated a strain of porcine deltacoronavirus (PDCoV) from the faeces of a diarrhoeic piglet, designated as CHN-HN-1601. The virus was found to cause severe diarrhea and villous atrophy of the small intestines in infected piglets.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Virology
Liqin Tu, Shuhua Wu, Danna Gao, Yong Liu, Yuelin Zhu, Yinghua Ji
Summary: The full-length genome of a Chinese isolate of Tomato mottle mosaic virus (ToMMV) was successfully isolated and cloned, leading to the development of an infectious cDNA clone that was able to infect both model and natural host plants. The infectious clone induced high infection rates in tomato plants, providing a valuable tool for further studies on the virus's gene function, pathogenicity, and virus-host interaction.
Article
Agriculture, Dairy & Animal Science
E. Guang-Xin, Yan-Bin Zhu, Wang-Dui Basang, Ri-Su Na, Yan-Guo Han, Yan Zeng
Summary: This study aimed to identify the CNV associated with litter size in Dazu black goats based on unpublished sequencing data. CBLB gene was found to potentially affect litter size, with Chr1_50215501 identified as an effective genetic marker for marker-assisted selection breeding. The study helps understand the molecular mechanisms related to goat litter size.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Zoology
Jie Li, Gaofu Wang, Xiaoyan Sun, Lin Fu, Peng Zhou, Hangxing Ren
Summary: This study cloned the goat KITL gene sequence and analyzed its different expression levels in goat tissue. The results showed that the goat KITL gene encoded a protein with high homology to other mammalian species. Additionally, the KITL gene was extensively expressed in goat tissue at different ages. The mRNA and protein levels of KITL were found to increase in goat skin with age, confirming its role in skin pigmentation.
PAKISTAN JOURNAL OF ZOOLOGY
(2023)
Article
Virology
Meixian Fu, Jinjin Bai, Shuang Gao, Zhangmei Chang, Xin Zhou, Jian-Er Long
Summary: This study describes a method for rapid rescue of infectious EV71 by using cells expressing T7 polymerase, which involves cloning the full-length EV71 genome with a T7 promoter and single transfection to efficiently rescue the virus. The rescued virus showed similar characteristics to the parental virus, and this approach circumvents the difficulty in in vitro transcription of long linear DNA.
ARCHIVES OF VIROLOGY
(2021)
Article
Plant Sciences
Xingan Hao, Shuang Song, Qinrong Zhong, Jamal-U-Ddin Hajano, Jie Guo, Yunfeng Wu
Summary: This study successfully constructed a full-length cDNA clone of BYDV-GAV and demonstrated its infectivity in wheat, confirming its ability to elicit typical symptoms. Using the infectious clone also revealed the involvement of viral protein P4 in cell-to-cell movement and stunting symptoms in wheat.
Article
Microbiology
Belen Carriqui-Madronal, Julie Sheldon, Mara Duven, Cora Stegmann, Karsten Cirksena, Emanuel Wyler, Francisco J. Zapatero-Belinchon, Florian W. R. Vondran, Gisa Gerold
Summary: This study reveals the critical role of ADAM10 as a host factor for HCV, supporting HCV entry and cell-to-cell spread through EGFR family member transactivation.
Article
Multidisciplinary Sciences
Wanyu Li, Yue Lan, Lei Wang, Lewei He, Ruixiang Tang, Megan Price, Bisong Yue, Zhenxin Fan
Summary: This study reassembled and analyzed the transcriptomes of the Heilongjiang brown frog, revealing tissue-specific gene expression. The oviduct showed enrichment of pathways and items related to protein synthesis and metabolism, while the skin contained a large number of antimicrobial peptides.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Zheng Xu, Qun Hu, Xupeng Zang, Chen Zhou, Dewu Liu, Guangbin Liu, Linjun Hong
Summary: Transcripts of uncertain coding potential (TUCP) in the goat endometrium during pregnancy recognition were investigated in this study. Bioinformatics analyses identified differentially expressed TUCPs, which were associated with endometrial remodeling, nutrient synthesis, and immune tolerance. These findings may aid in selecting candidate genes for further functional analyses related to embryo implantation.
DNA AND CELL BIOLOGY
(2021)
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.