Article
Environmental Sciences
Jing Wang, Chen Liu, Shu-Ping Wang, Tian-Xu Zhang, Jing-Yi Chen, Quan Zhou, Yin Hou, Zhen-Guang Yan
Summary: This study investigated the toxic effects and underlying mechanisms of decabromodiphenyl ether (BDE-209) exposure on the intestinal tract using adult male zebrafish as a model. The results showed that BDE-209 exposure caused damage to intestinal structure and barrier function, and promoted oxidative stress, inflammatory response, apoptosis, and disorders in steroid and lipid metabolism. Mechanistically, BDE-209 induced intestinal inflammation through the NF kappa B signaling pathway, and might induce apoptosis through the p53-Bax/Bcl2-Caspase3 pathway. BDE-209 also disrupted cholesterol synthesis and caused lipid metabolism disorders. Furthermore, BDE-209 stress reduced the richness and diversity of intestinal microbiota and decreased the abundance of probiotics.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Marion Garofalo, Delphine Payros, Eric Oswald, Jean-Philippe Nougayrede, Isabelle P. Oswald
Summary: This study found that the dose of DON found in food can exacerbate DNA damage caused by etoposide, cisplatin, and phleomycin, but not the DNA damage caused by de-epoxy-deoxynivalenol (DOM-1). Ribotoxicity plays a key role in exacerbating DNA damage.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Toxicology
Ji-Eun Seo, Joshua Z. Yu, Hannah Xu, Xilin Li, Aisar H. Atrakchi, Timothy J. McGovern, Karen L. Davis Bruno, Nan Mei, Robert H. Heflich, Xiaoqing Guo
Summary: This study evaluated the genotoxicity of eight nitrosamines using metabolically competent human HepaRG cells. The HepaRG spheroids, which had higher levels of cytochrome P450 activity compared to monolayer cells, were used along with conventional 2D cultures. The results suggest that HepaRG spheroids may provide a sensitive, human-based cell system for evaluating the genotoxicity of nitrosamines.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Sneh M. Toprani, Dimitrios Bitounis, Qiansheng Huang, Nathalia Oliveira, Kee Woei Ng, Chor Yong Tay, Zachary D. Nagel, Philip Demokritou
Summary: The potential genotoxic effects of engineered nanomaterials (ENMs) on DNA repair pathways have been investigated using a fluorescence multiplex-host-cell reactivation (FM-HCR) assay. Results showed that some ENMs may enhance DNA repair capacity while others suppress it. This method can be a valuable part of a multitier, in vitro hazard assessment of ENMs, providing insights into the interplay between ENM properties, DNA repair efficiency, and genomic stability.
Article
Environmental Sciences
Felipe de Araujo Nascimento, Daniela de Melo e Silva, Thays Millena Alves Pedroso, Jheneffer Sonara Aguiar Ramos, Michelle Rocha Parise
Summary: Occupational exposure to pesticides in farmers results in significant DNA damage, showing a notable impact and variability in the studies. Smokers who are occupationally exposed to pesticides have slightly reduced DNA damage compared to non-smokers, indicating some genetic toxicity. The study demonstrates a higher risk of occupationally exposed rural workers developing related diseases due to the genotoxic effect of pesticides.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ireneusz Litwin, Seweryn Mucha, Ewa Pilarczyk, Robert Wysocki, Ewa Maciaszczyk-Dziubinska
Summary: In this study, it was found that trivalent antimony can cause various forms of DNA damage, including replication and oxidative DNA damage, and affect the activation of DNA damage checkpoints and formation of recombination repair centers in the model organism Saccharomyces cerevisiae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
F. Akcha, C. Cahuc, J. Rouxel, C. Munschy, Y. Aminot, T. Chouvelon, K. Mahe, H. Budzinski, A. Mauffret
Summary: Telomeres are suggested as a biomarker for cellular aging, stress exposure, and lifespan in humans, and a q-PCR protocol was used to measure Telomere Length (TL) in European flounder. The study found no correlation between TL and DNA damage or measured contaminant concentrations in the flounder, but further research is needed to determine the potential impact of chemical pollution on flatfish telomeres.
MARINE POLLUTION BULLETIN
(2021)
Article
Biochemistry & Molecular Biology
Aline H. Sousa, Joao P. G. Pereira, Allan C. Malaquias, Fernanda do E. S. Sagica, Edivaldo H. C. de Oliveira
Summary: Mercury is widely used in industrial processes and improper disposal of waste or products containing this metal can have significant negative impacts on ecosystems and living organisms, including humans. Methylmercury, a highly toxic organic compound, can cause neurological and developmental impairments. Recent research has shown that mercurial compounds can also cause genotoxic effects, such as disrupting mitotic spindle and causing chromosome loss, which are associated with the neurological effects of mercury. This study aimed to investigate the cytotoxic and genotoxic potential of methylmercury in neuron and glia cell lines. The findings showed that glial cells had a higher tolerance to methylmercury compared to neuronal cells, leading to higher intracellular accumulation of mercury and the occurrence of genotoxic effects.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Article
Environmental Sciences
Tuanwei Wang, Yu Meng, Yuting Tu, Guanghui Zhang, Kan Wang, Shiyang Gong, Yunxia Zhang, Tongshuai Wang, Anqi Li, David C. Christiani, William Au, Zhao-lin Xia
Summary: Studies have shown that lead exposure can cause genotoxicity, and one possible mechanism is through DNA methylation. This study aimed to investigate the association between DNA methylation, lead exposure, and genotoxicity. Blood lead levels and genotoxic biomarkers were analyzed in lead-exposed workers, and the methylation levels of specific genes were measured. The results showed that lead exposure induced changes in DNA methylation, which mediated lead-induced genotoxicity.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Xixi Li, Guili Song, Yasong Zhao, Jing Ren, Qing Li, Zongbin Cui
Summary: The study revealed the essential role of SMC2 in zebrafish liver development, as its depletion led to a small liver phenotype, potentially linked to the activation of the p53-dependent apoptotic pathway.
Article
Biochemistry & Molecular Biology
Galina N. Chelomina, Sergey P. Kukla, Viktor P. Chelomin, Pham N. Doanh
Summary: Parasite infection can lead to DNA damage, potentially involving oxidative stress and cancer development; these research findings are important for understanding the pathogenesis mechanisms of parasitic diseases.
Article
Biochemistry & Molecular Biology
Ievgeniia Gazo, Roman Franek, Radek Sindelka, Ievgen Lebeda, Sahana Shivaramu, Martin Psenicka, Christoph Steinbach
Summary: Sterlet embryos show varying sensitivity to different genotoxicants, with some inducing DNA damage and activating DDR pathways, while others causing DNA damage without activating known DDR pathways, with effects also being stage-specific in embryo development. This study provides foundational information for future investigation of sterlet DDR pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Donald H. Atha, Vytas Reipa
Summary: This report describes several methods that could be considered for the production of reference materials for the quantitative measurement of DNA damage in mammalian cells. The focus is on potentially useful methods for assessing DNA damage, particularly DNA strand breaks, in mammalian cells. The advantages, limitations, and concerns with respect to reference material development are discussed. In conclusion, strategies for developing candidate DNA damage reference materials are outlined for adoption by research laboratories in various applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Kangying Wu, Qixing Zhou, Shaohu Ouyang
Summary: Graphene family nanomaterials have diverse applications and potential genotoxic effects on DNA, with mechanisms classified into direct and indirect genotoxicity. Factors such as physicochemical properties, exposure dose, and time influence the genotoxicity of GFNs, requiring further research on testing methods and response mechanisms.
Article
Environmental Sciences
Filomena Mottola, Concetta Iovine, Marianna Santonastaso, Vincenzo Carfora, Severina Pacifico, Lucia Rocco
Summary: The contamination of the environment by nanoparticles and drugs has become a controversial issue. This study focuses on the harmful consequences of exposure to antibiotics and nanoparticles, both individually and in combination, in the aquatic environment. The research was conducted on zebrafish, and the results show that exposure to nanoparticles and antibiotics together increases DNA damage and decreases cell viability.
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.