Article
Cell Biology
Loic Fort, Vivian Gama, Ian G. Macara
Summary: This study revealed that pluripotent stem cells undergo apoptosis and an epithelial-to-mesenchymal transition during cardiomyocyte conversion. Apoptosis is triggered by the absence of bFGF in the differentiation medium, while EMT requires the induction of SNAI1 and SNAI2 transcription factors. Additionally, nucleotides play a crucial role in the mesoderm specification process.
NATURE CELL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Emily L. Miedzybrodzka, Rachel E. Foreman, Van B. Lu, Amy L. George, Christopher A. Smith, Richard G. Kay, Deborah A. Goldspink, Frank Reimann, Fiona M. Gribble
Summary: This study provides a comprehensive understanding of the regulation of motilin secretion in human duodenal M-cells, identifying key receptors involved in this process.
MOLECULAR METABOLISM
(2021)
Article
Biology
Sydney Trask, Jeffrey S. Mogil, Fred J. Helmstetter, Cheryl L. Stucky, Katelyn E. Sadler
Summary: The mechanisms underlying the transition from acute to chronic pain are still unclear, but it may involve the persistence or strengthening of pain memories acquired through associative learning. Recent studies have shown that contextual cues play a critical role in regulating pain memory. Animals and humans exhibit increased pain sensitivity in environments associated with painful experiences. This study suggests that pain perception and activation of endogenous opioid systems can be modified through psychological association with environmental cues.
Article
Cell Biology
Jean-Pierre Bikorimana, Jamilah Abusarah, Natasha Salame, Nehme El-Hachem, Riam Shammaa, Moutih Rafei
Summary: The use of mesenchymal stromal cells has revolutionized modern medicine, with their ability to modulate immune responses and promote humoral immunity. Their therapeutic potency is dependent on the balance between efferocytosis-stimulating and -inhibiting signals.
Article
Multidisciplinary Sciences
Dennis J. Weingarten, Amita Shrestha, Kessa Juda-Nelson, Sarah A. Kissiwaa, Evan Spruston, Skyler L. Jackman
Summary: Sustained neuronal activity requires rapid replenishment of synaptic vesicles to maintain reliable synaptic transmission. Synaptotagmin-3 (SYT3) has been identified as the high-affinity Ca2+ sensor responsible for accelerating vesicle replenishment and promoting short-term synaptic plasticity by promoting the transition of vesicles from loosely docked to tightly docked states.
Article
Chemistry, Multidisciplinary
D. Massana Roquero, P. Bollella, O. Smutok, E. Katz, A. Melman
Summary: This biocompatible drug delivery system shows a highly sensitive stimuli-responsive behavior to hydrogen peroxide, effectively releasing encapsulated proteins through oxidative degradation of boronate esters. The release can be triggered by low concentrations of hydrogen peroxide and can also be induced by incorporating oxidase enzymes in the presence of their substrates, serving as biomarkers for physiological disorders.
MATERIALS TODAY CHEMISTRY
(2021)
Article
Chemistry, Analytical
Cecilia Villegas-Novoa, Yuli Wang, Christopher E. Sims, Nancy L. Allbritton
Summary: This paper describes an optimized analytical assay pipeline for screening compounds to modulate L-cell signaling. The pipeline utilizes a human intestinal tissue construct enriched in L-cells and standard antibody-based GLP-1 assays to quantify GLP-1 secretion. Additionally, by adding specific compounds and altering the culture conditions, the number of L-cells is increased to improve the signal-to-noise ratio of conventional enzyme-linked immunoassays.
ANALYTICAL CHEMISTRY
(2022)
Review
Endocrinology & Metabolism
Abdelgadir M. Homeida, Mohamed A. Homeida, Ebtesam A. Al-Suhaimi
Summary: The timing of food intake is crucial for regulating circadian rhythms in humans and animals. Intestinal enteroendocrine cells produce incretin hormones in response to food intake, which stimulate insulin secretion and control body weight and energy expenditure. During pregnancy, the expansion of beta cells, risk of gestational diabetes mellitus, and excessive weight gain can be addressed by timing food intake. This review focuses on the circadian rhythms and biological actions of enteroendocrine hormones, particularly in relation to pregnancy, including food intake, gut circadian rhythms, secretion of enteroendocrine peptides, and their effects during pregnancy.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Engineering, Biomedical
Fengying Shao, Jianyu Han, Zhaoyan Tian, Zhi Wang, Songqin Liu, Yafeng Wu
Summary: Utilizing the endogenous Ca2+ to upregulate intramitochondrial Ca2+ level is a promising strategy for mitochondrial dysfunction-mediated tumor therapy. However, the uncontrollability and non-selectivity of endogenous Ca2+ transport limit the amount of Ca2+ entering mitochondria. In this study, a smart intracellular Ca2+ directional transport channel was fabricated to selectively transport endogenous Ca2+ from endoplasmic reticulum (ER) to mitochondria, achieving significant upregulation of intramitochondrial Ca2+ level and efficient tumor inhibition through synergetic ROS generation and mitochondrial Ca2+ overloading.
Article
Biology
Miaomiao Wang, Yang Wang, Liu Yang, Xing Du, Qifa Li
Summary: This study reveals a novel mechanism of action for nuclear lncRNAs and shows that it acts as a competing endogenous RNA (ceRNA), which is also a common function of lncRNAs in the cytoplasm. The study also identifies a potential endogenous small activating RNA (saRNA) for improving female fertility and treating female infertility.
Article
Neurosciences
Dominika Labuz, Melih O. Celik, Viola Seitz, Halina Machelska
Summary: Interleukin-4 (IL-4) is an anti-inflammatory cytokine that can alleviate pain by releasing opioid peptides from infiltrating macrophages at injured nerves. This newly discovered mechanism involves PKA-mediated, PI3K-mediated, ryanodine receptor-mediated, and intracellular Ca21-mediated release from M1 macrophages of opioid peptides, which activate peripheral opioid receptors in injured tissue. Targeting the IL-4-opioid system in peripheral damaged tissue may offer a potential therapeutic approach with fewer central and systemic side effects.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biology
Di Peng, Koji Ando, Melina Hussmann, Marleen Gloger, Renae Skoczylas, Naoki Mochizuki, Christer Betsholtz, Shigetomo Fukuhara, Stefan Schulte-Merker, Nathan D. Lawson, Katarzyna Koltowska
Summary: In zebrafish, arterial mural cells have been identified as a crucial source of signals that guide the migration and survival of lymphatic endothelial cells (LECs), promoting the establishment of the lymphatic vascular network. The cooperative function of chemokine and growth factor signaling, particularly through the VEGFC-VEGFR3 pathway, plays a key role in LEC migration.
Article
Biology
Simon Desch, Petra Schweinhardt, Ben Seymour, Herta Flor, Susanne Becker, Markus Ploner
Summary: Active decision-making and unpredictability enhance the perception of pain relief, and this effect is more pronounced in individuals with high novelty-seeking traits. Dopaminergic signaling plays a key role in modulating the perception of pain relief, while the effect of opioidergic manipulation is not significant.
Article
Multidisciplinary Sciences
Jennifer P. Ditano, Katelyn L. Donahue, Laura J. Tafe, Charlotte F. McCleery, Alan Eastman
Summary: DNA damage activates cell cycle checkpoint proteins ATR and CHK1 to arrest cell cycle progression, providing time for repair and recovery. Inhibitors of ATR and CHK1 enhance damage-induced cell death, while both ATRi and CHK1i can elicit cytotoxicity in some cell lines. Sensitivity is associated with endogenous replication stress, with premature hyper-activation of CDK2 playing a key role in sensitivity to ATRi and CHK1i as single agents.
SCIENTIFIC REPORTS
(2021)
Review
Psychiatry
Ardavan Mohammad Aghaei, Alexandra Saali, Maria Alejandra Canas, Jeremy Weleff, Deepak Cyril D'Souza, Gustavo A. Angarita, Anahita Bassir Nia
Summary: This paper reviews the clinical and preclinical literature on the dysregulation of the endocannabinoid system (ECS) after exposure to opioids. The results show that opioids disrupt various ECS components in specific brain regions. However, the literature results are heterogeneous and contradictory, suggesting the need for further research to determine the effects of opioids on ECS components in different brain regions.
PSYCHIATRY RESEARCH
(2023)
Article
Immunology
Pu Feng, Jinghua Chai, Huilan Yi, Kevin Redding, Robert F. Margolskee, Liquan Huang, Hong Wang
BRAIN BEHAVIOR AND IMMUNITY
(2018)
Article
Neurosciences
Ryusuke Yoshida, Shingo Takai, Keisuke Sanematsu, Robert F. Margolskee, Noriatsu Shigemura, Yuzo Ninomiya
Article
Multidisciplinary Sciences
Weiwei Lei, Wenwen Ren, Makoto Ohmoto, Joseph F. Urban, Ichiro Matsumoto, Robert F. Margolskee, Peihua Jiang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Genetics & Heredity
Yumei Qin, Sunil K. Sukumaran, Masafumi Jyotaki, Kevin Redding, Peihua Jiang, Robert F. Margolskee
Article
Multidisciplinary Sciences
Ya-Wen Du, Qun Liu, Xiao-Cui Luo, Dong-Xiao Zhao, Jian-Bo Xue, Pu Feng, Robert F. Margolskee, Hong Wang, Liquan Huang
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Behavioral Sciences
Yi Wang, Amanda L. Zajac, Weiwei Lei, Carol M. Christensen, Robert F. Margolskee, Cedric Bouysset, Jerome Golebiowski, Huabin Zhao, Sebastien Fiorucci, Peihua Jiang
Article
Multidisciplinary Sciences
Shingo Takai, Yu Watanabe, Keisuke Sanematsu, Ryusuke Yoshida, Robert F. Margolskee, Peihua Jiang, Ikiru Atsuta, Kiyoshi Koyano, Yuzo Ninomiya, Noriatsu Shigemura
Article
Multidisciplinary Sciences
Xin Zheng, Marco Tizzano, Kevin Redding, Jinzhi He, Xian Peng, Peihua Jiang, Xin Xu, Xuedong Zhou, Robert F. Margolskee
NATURE COMMUNICATIONS
(2019)
Article
Behavioral Sciences
Shan Feng, Leyitha Achoute, Robert F. Margolskee, Peihua Jiang, Hong Wang
Article
Physiology
Keiko Yasumatsu, Tadahiro Ohkuri, Ryusuke Yoshida, Shusuke Iwata, Robert F. Margolskee, Yuzo Ninomiya
Article
Gastroenterology & Hepatology
Megan T. Hoffman, Samantha B. Kemp, Daniel J. Salas-Escabillas, Yaqing Zhang, Nina G. Steele, Stephanie The, Daniel Long, Simone Benitz, Wei Yan, Robert F. Margolskee, Filip Bednar, Marina Pasca di Magliano, Hui-Ju Wen, Howard C. Crawford
Summary: This study demonstrates that inhibiting GNAT3 can increase the release of tumor-promoting cytokines, alter the MDSC population, and promote the progression of metastatic PDA.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Multidisciplinary Sciences
Xiaoli Lin, Chanyi Lu, Makoto Ohmoto, Katarzyna Choma, Robert F. Margolskee, Ichiro Matsumoto, Peihua Jiang
Summary: The neuron-supplied niche factor, R-spondin-2, plays a crucial role in taste bud cell regeneration and maintenance by promoting the generation of differentiated taste cells. It can substitute for neuronal input to maintain taste bud cells and tissues. The absence of R-spondin in culture medium inhibits the generation of taste bud cells ex vivo.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Developmental Biology
Yumei Qin, Sunil K. Sukumaran, Robert F. Margolskee
Summary: In mammals, the transcription factor Nkx2-2 plays a crucial role in the development of the taste system, specifically in type III taste cells; Nkx2-2 is expressed in endoderm-derived taste papillae and cells expressing Nkx2-2 differentiate into type III taste cells; Nkx2-2-expressing cells are committed to the type III lineage in the posterior tongue, indicating a key difference in cell lineage specification between ectoderm- and endoderm-derived taste fields.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Toshiaki Yasuo, G. Craig Wood, Xin Chu, Peter Benotti, Christopher D. Still, David D. K. Rolston, Robert F. Margolskee, Yuzo Ninomiya, Peihua Jiang
Summary: This study shows that taste-signaling proteins are dysregulated in the jejunal tissues of patients with extreme obesity, suggesting their involvement in obesity.
JOURNAL OF ORAL BIOSCIENCES
(2022)
Article
Biology
Nanako Atsumi, Keiko Yasumatsu, Yuriko Takashina, Chiaki Ito, Norihisa Yasui, Robert F. Margolskee, Atsuko Yamashita
Summary: The perception of salt taste is complex, with NaCl at low and high concentrations being preferentially or aversively perceived through different pathways. Cl- is believed to participate in taste sensation through an unknown mechanism. This study found that Cl- ions specifically bind to the ligand-binding domain (LBD) of taste receptor type 1 (T1r), inducing a conformational change in the receptor. The oral application of Cl- to mice increased the activity of taste nerves connected to T1r-expressing taste cells and influenced their behavioral preferences, which could be attenuated by blocking T1r or knocking out T1r3.