Article
Medical Laboratory Technology
Bennett Oh Vic Shum, Carel Jacobus Pretorius, Letitia Min Fen Sng, Ilya Henner, Paulette Barahona, Emre Basar, Jim McGill, Urs Wilgen, Anna Zournazi, Lilian Downie, Natalie Taylor, Liam Cheney, Sylvania Wu, Natalie Angela Twine, Denis Carolin Bauer, Gerald Francis Watts, Akash Navilebasappa, Kishore Rajagopal Kumar, Jacobus Petrus Johannes Ungerer, Glenn Bennett
Summary: The study found that newborn screening can be expanded using targeted gene sequencing (TGS), which is a feasible and cost-effective method. The TGS assay had a high sensitivity (>99%) and specificity (100%) with a turnaround time of 7 to 10 days and a maximum batch size of 1536 samples. This approach can increase the number of conditions screened and improve the effectiveness of newborn screening programs.
CLINICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Raphael Rodriguez, Yamuna Krishnan
Summary: This article reviews the historical development of next-generation sequencing chemistry, highlighting the significant impact of the underlying chemical principles on biology.
NATURE BIOTECHNOLOGY
(2023)
Article
Microbiology
Tara E. Ness, Lennard Meiwes, Alexander Kay, Rojelio Mejia, Christoph Lange, Maha Farhat, Anna Mandalakas, Andrew DiNardo
Summary: This study compares different DNA extraction techniques for Mycobacterium tuberculosis from pediatric stool samples, providing an important starting point for researchers to isolate high-quality DNA and optimize protocols. The study highlights the importance of optimizing DNA extraction from stool samples to ensure accurate diagnosis and drug susceptibility information.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemical Research Methods
Josh Moore, Chris Allan, Sebastien Besson, Jean-Marie Burel, Erin Diel, David Gault, Kevin Kozlowski, Dominik Lindner, Melissa Linkert, Trevor Manz, Will Moore, Constantin Pape, Christian Tischer, Jason R. Swedlow
Summary: The rapid pace of innovation in biological imaging has led to a lack of a standardized data format. By combining established open formats with a next-generation file format and a common metadata format, bioimaging data can be made findable, accessible, interoperable, and reusable.
Article
Chemistry, Multidisciplinary
Tiandi Wu, Kevin D. Moeller
Summary: Paired electrochemical reactions allow for optimization of atom and energy economy of oxidation and reduction reactions by adjusting the potentials at both electrodes, with substrate matching not being necessary. Any oxidation reaction can theoretically be paired with any reduction reaction.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Astronomy & Astrophysics
D. Camarena, V. Marra, Z. Sakr, S. Nesseris, A. Da Silva., J. Garcia-Bellido., P. Fleury., L. Lombriser, M. Martinelli, C. J. A. P. Martins, J. Mimoso, D. Sapone, C. Clarkson, S. Camera, C. Carbone, S. Casas, S. Ilic, V. Pettorino, I. Tutusaus, N. Aghanim, B. Altieri, A. Amara, N. Auricchio, M. Baldi, D. Bonino, E. Branchini, M. Brescia, J. Brinchmann, G. P. Candini, V. Capobianco, J. Carretero, M. Castellano, S. Cavuoti, A. Cimatti, R. Cledassou, G. Congedo, L. Conversi, Y. Copin, L. Corcione, F. Courbin, M. Cropper, H. Degaudenzi, F. Dubath, C. A. J. Duncan, X. Dupac, S. Dusini, A. Ealet, S. Farrens, P. Fosalba, M. Frailis, E. Franceschi, M. Fumana, B. Garilli, B. Gillis, C. Giocoli, A. Grazian, F. Grupp, S. V. H. Haugan, W. Holmes, F. Hormuth, A. Hornstrup, K. Jahnke, A. Kiessling, R. Kohley, M. Kunz, H. Kurki-Suonio, P. B. Lilje, I. Lloro, O. Mansutti, O. Marggraf, F. Marulli, R. Massey, M. Meneghetti, E. Merlin, G. Meylan, M. Moresco, L. Moscardini, E. Munari, S. M. Niemi, C. Padilla, S. Paltani, F. Pasian, K. Pedersen, G. Polenta, M. Poncet, L. Popa, L. Pozzetti, F. Raison, R. Rebolo, J. Rhodes, G. Riccio, H. -w. Rix, E. Rossetti, R. Saglia, B. Sartoris, A. Secroun, G. Seidel, C. Sirignano, G. Sirri, L. Stanco, C. Surace, P. Tallada-Crespi, A. N. Taylor, I. Tereno, R. Toledo-Moreo, F. Torradeflot, E. A. Valentijn, L. Valenziano, Y. Wang, G. Zamorani, J. Zoubian, S. Andreon, V. Scottez, M. Tenti
Summary: This paper investigates the precision and ability of future surveys to test the Copernican principle and detect possible violations. The results show that next-generation surveys, such as Euclid, will thoroughly test homogeneity at large scales and tighten the constraints on possible violations of the Copernican principle.
ASTRONOMY & ASTROPHYSICS
(2023)
Article
Multidisciplinary Sciences
Wenqian Song, Nan Xiao, Shihang Zhou, Weijian Yu, Ni Wang, Linnan Shao, Ying Duan, Mei Chen, Lingzi Pan, Yuexin Xia, Li Zhang, Ming Liu
Summary: This study demonstrates the high accuracy of Y-chromosome mini-STR for non-invasive prenatal paternity testing (NIPPT) and highlights its potential for forensic and medical applications.
Article
Genetics & Heredity
Yusra Alyafee, Abeer Al Tuwaijri, Qamre Alam, Muhammad Umair, Shahad Haddad, Mashael Alharbi, Maryam Ballow, Mohammed Al Drees, Abdulkareem AlAbdulrahman, Aziza Al Khaldi, Majid Alfadhel
Summary: This study reported the successful implementation of an in-house NIPT screening service in Saudi Arabia, showing high accuracy and sensitivity to detect high-risk cases. This technique could potentially change the common screening practice for pregnant women in Saudi Arabia.
FRONTIERS IN GENETICS
(2021)
Article
Chemistry, Multidisciplinary
Richard Houska, Marvin Bjorn Stutz, Oliver Seitz
Summary: The study presents a reaction system for synthesizing Bcr-Abl tyrosine kinase inhibitors in water using native chemical ligation to form benzanilides. This method was successfully applied for the synthesis of thiolated ponatinib and GZD824 derivatives, with only a moderate effect on the affinity for Abl1 kinase. Nucleic acid-templated benzanilide formation could potentially find application in DNA-encoded combinatorial libraries for drug discovery.
Review
Chemistry, Multidisciplinary
Raaju Sundhar Arul Saravanan, Keyru Serbara Bejigo, Sang-Jae Kim
Summary: Nanomaterials are being explored as potential cathode materials in mainstream lithium-ion batteries, but research is still in the early stages. The synthesis of transition metal oxide nanomaterials and their performance as cathodes in Li-ion batteries are discussed in this review. The aim is to provide researchers with an understanding of the current challenges and opportunities in working with nanomaterials for next-generation batteries.
MATERIALS CHEMISTRY FRONTIERS
(2023)
Article
Biochemistry & Molecular Biology
Songchang Chen, Lanlan Zhang, Jiong Gao, Shuyuan Li, Chunxin Chang, Yiyao Chen, Hongjun Fei, Junyu Zhang, Yanlin Wang, Hefeng Huang, Chenming Xu, Daru Lu
Summary: Non-invasive prenatal testing (NIPT) is effective for common fetal trisomies, but the usefulness of cell-free DNA testing for other chromosomal abnormalities is not well understood. Increasing the read depth in next-generation sequencing (NGS) improves positive CNV detection rates and reduces false positives, potentially making NIPT by NGS an accurate method for analyzing fetal chromosomes and reducing birth defects.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Gastroenterology & Hepatology
Jordan K. Voss, Derek W. Ebner, Keli N. Burger, Douglas W. Mahoney, Mary E. Devens, Kari L. Lowrie, John B. Kisiel
Summary: Multitarget stool DNA (mt-sDNA) testing is an effective screening method for colorectal cancer. The positive rate and positive predictive value (PPV) of mt-sDNA were examined in a second round of testing and found to be similar to those in the first round. These findings confirm the usefulness of mt-sDNA for CRC screening.
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Oncology
Sebastian Mondaca, Emily S. Lebow, Azadeh Namakydoust, Pedram Razavi, Jorge S. Reis-Filho, Ronglai Shen, Michael Offin, Hai-Yan Tu, Yonina Murciano-Goroff, Chongrui Xu, Alex Makhnin, Andres Martinez, Nick Pavlakis, Stephen Clarke, Malinda Itchins, Adrian Lee, Andreas Rimner, Daniel Gomez, Gaetano Rocco, Jamie E. Chaft, Gregory J. Riely, Charles M. Rudin, David R. Jones, Mark Li, Tristan Shaffer, Seyed Ali Hosseini, Caterina Bertucci, Lee P. Lim, Alexander Drilon, Michael F. Berger, Ryma Benayed, Maria E. Arcila, James M. Isbell, Bob T. Li
Summary: Liquid biopsy for plasma ctDNA NGS is effective in detecting ALK fusions with shorter turnaround time compared to tissue testing and high concordance rate. ctDNA can also match patients to ALK-directed therapy and detect mechanisms of treatment resistance.
Review
Oncology
Jeremy Setton, Michael Zinda, Nadeem Riaz, Daniel Durocher, Michal Zimmermann, Maria Koehler, Jorge S. Reis-Filho, Simon N. Powell
Summary: Synthetic lethality (SL) provides a theoretical framework for targeting loss-of-function mutations in tumor suppressor genes and DNA repair genes, as well as amplification and/or overexpression of genes that cannot be directly targeted. The emergence of next generation tumor-specific alterations targetable through SL from high-throughput CRISPR technology presents new opportunities for drug development, but also brings important challenges in developing optimal predictive biomarkers.
Review
Engineering, Biomedical
William Ho, Mingzhu Gao, Fengqiao Li, Zhongyu Li, Xue-Qing Zhang, Xiaoyang Xu
Summary: Nucleic acid vaccines hold promise as an immunization method for combating pandemics, with nanoparticles being a top candidate for successful DNA/mRNA vaccine delivery due to their unique properties.
ADVANCED HEALTHCARE MATERIALS
(2021)